Autoimmune Complement Fixation Test in Endogenous Uveitis

Hallett, Joseph W.; Wolkowicz, Michael I.; Leopold, Irving H.; Canamucio, C.; Wijewski, Eugenia

doi:10.1001/archopht.1962.00960030172005

Cited by 32 publications

(4 citation statements)

References 5 publications

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“…1 However, in spite of intensive investigation, specific <etiological agent(s) have rarely been demonstrated, and associa tions with other systemic diseases have been shown in only 50% of cases.2-4 A large prop ortion of these cases present evidence of an immunological disturbance indicating that immune mechanisms are involved in initiat ing and/or establishing the chronicity of the disease and its tendency to recur. [5][6][7][8][9] Experi mental autoimmune uveitis (EAU) induced by retinal antigens, particularly S-antigen is a useful animal model of chronic intraocular inflammation. Besides uveitis, S-antigen in duces an inflammatory reaction in the pineal gland thereby demonstrating another link between the pineal gland and the eye.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulation of Experimental Autoimmune Uveitis using a rat anti retinal S-antigen specific monoclonal antibody: Evidence for a species difference

Dua

Liversidge²,

Forrester³

1989

Eye

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SummaryExperimental Autoimmune Uveitis (EAU) induced by retinal antigens, particularly S-antigen, forms a useful model of human chronic intraocular inflammation particularly endogenous post erior uveitis. It provides a means of assessing the efficacy of various agents used in the control of such inflammation. We induced an autoimmune uveitis and its associated pinealitis in Dun kin-Hartley guinea pigs and Lewis rats by inoculating them with bovine retinal S-antigen. In rats, induction of EAU but not of Experimental Autoimmune Pinealitis (EAP) could be pre vented by the administration of S-antigen specific rat monoclonal antibody simultaneously with the S-antigen. Inhibition of EAU was accompanied by significantly raised levels of anti-S antibodies during the first two weeks post-immunisation. In contrast, the same monoclonal antibody failed to inhibit both EAU and EAP in guinea pigs. Immunocytochemical staining of rat tissues for lymphocyte subsets, monocytes and macrophages showed that eyes of monoc lonal antibody treated animals contained no immunocompetent inflammatory cells unless they also had clinical signs of inflammation. In contrast, the inflammatory exudate in the pineal glands of both treated and untreated animals contained equal numbers of infiltrating lympho cytes and monocytes in the same relative proportions. These results indicate that the inhibitory effect of the monoclonal antibody S2.4.CS is directed towards the effector arm of the immune mediated cytotoxic response. A possible mechanism by which the antibody may be preferen tially inhibiting the inflammatory response in the eyes but not in the pineal glands of rats, is suggested.Chronic intraocular inflammation is an important cause of ocular morbidity and vis ual loss particularly among young adults. Several exogenous and endogenous factors have been identified in the

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Section: Discussionmentioning

confidence: 99%

Immunomodulation of Experimental Autoimmune Uveitis using a rat anti retinal S-antigen specific monoclonal antibody: Evidence for a species difference

Dua

Liversidge²,

Forrester³

1989

Eye

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“…Complement-Fixation Tests.-These were carried out by the method of Hallett, Wolkowicz, Leopold, Canamucio, and Wijewski (1962). The antigen used was prepared from bovine choroidal tissue.…”

Section: Serological Studies In Sympathetic Ophthalmitismentioning

confidence: 99%

Serological Studies in Sympathetic Ophthalmitis

Mills¹,

Shedden²

1965

British Journal of Ophthalmology

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“…Bovine and Normal Human Uveal Antigens.-Extracts containing crude uveal pigment were prepared from fresh bovine and normal human choroid. Tissue was ground by hand in sterile isotonic saline, subjected to autolysis at 37 C, and then centri fuged, according to a modification of the tech¬ nique described by Hallett et al 5 The resulting supernatant fluid was then lyophilized and stored at -20 C. Antigen for gel diffusion-studies was prepared as needed by dissolving 10 mg of the lyophilized preparation per cubic centimeter of dis¬ tilled water. This same solution was diluted 1 :3 for use in the passive hemagglutination studies.…”

mentioning

confidence: 99%