Autoimmunity against human tropomyosin isoforms in ulcerative colitis: Localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs

Mirza, Zafar K.; Sastri, Bhagyalakshmi; Lin, Jim Jung‐Ching; Amenta, Peter S.; Das, Kiron M.

doi:10.1097/01.mib.0000231573.65935.67

Cited by 22 publications

(18 citation statements)

References 37 publications

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“…This modulates the characteristic recurring cycles of chronic inflammation in Crohn's disease. Likewise, ulcerative colitis has an autoimmune component different to that of Crohn's disease [63,64] in that isoforms of human tropomyosin, capable of inducing autoantibodies and T-cell responses, have been observed in ulcerative colitis [65]. Similarly, autoimmunity could explain some elements of organ cross-talk in inflammatory disease.…”

Section: Respiratory Tractmentioning

confidence: 99%

Inflammatory bowel diseases, chronic liver diseases and the lung

Rodríguez-Roisin

Bartolome

Huchon³

et al. 2016

Eur Respir J

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This review is devoted to the distinct associations of inflammatory bowel diseases (IBD) and chronic liver disorders with chronic airway diseases, namely chronic obstructive pulmonary disease and bronchial asthma, and other chronic respiratory disorders in the adult population. While there is strong evidence for the association of chronic airway diseases with IBD, the data are much weaker for the interplay between lung and liver multimorbidities. The association of IBD, encompassing Crohn's disease and ulcerative colitis, with pulmonary disorders is underlined by their heterogeneous respiratory manifestations and impact on chronic airway diseases. The potential relationship between the two most prevalent liver-induced pulmonary vascular entities, i.e. portopulmonary hypertension and hepatopulmonary syndrome, and also between liver disease and other chronic respiratory diseases is also approached. Abnormal lung function tests in liver diseases are described and the role of increased serum bilirubin levels on chronic respiratory problems are considered. @ERSpublications Lung-gut cross-talk: the association of IBD with respiratory multimorbitidies, including COPD and bronchial asthma

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Section: Respiratory Tractmentioning

confidence: 99%

Inflammatory bowel diseases, chronic liver diseases and the lung

Rodríguez-Roisin

Bartolome

Huchon³

et al. 2016

Eur Respir J

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“…It may also be externalized on colonic epithelial cells and, as a result, accessible to the effector immune response. 23,27 Emerging evidence suggests that hTM5, previously denoted P40 by some investigators, is an autoantigen in UC. 4,10,12,27,29,36 Patients with UC have been shown to harbor both humoral and T-cell-mediated immune responses to hTM5, and hTM5-specific autoantibodies derived from the sera of UC patients cause destruction of colonic epithelial cells via a complimentmediated process.…”

Section: Discussionmentioning

confidence: 99%

“…23,27 Emerging evidence suggests that hTM5, previously denoted P40 by some investigators, is an autoantigen in UC. 4,10,12,27,29,36 Patients with UC have been shown to harbor both humoral and T-cell-mediated immune responses to hTM5, and hTM5-specific autoantibodies derived from the sera of UC patients cause destruction of colonic epithelial cells via a complimentmediated process. 10,12,29,37 Biancone et al reported that autoantibodies against both hTM1 and hTM5 were significantly elevated in UC patients compared with CD and normal controls, and several other studies have shown that increased colonic hTM5 and elevated antihTM5 IgG titers in sera of UC patients correlate with severity of disease.…”

Section: Discussionmentioning

confidence: 99%

Alterations in the Immunohistochemical Expression of Das-1 and CG-3 in Colonic Mucosal Biopsy Specimens Helps Distinguish Ulcerative Colitis From Crohn Disease and From Other Forms of Colitis

Yantiss

Das

Farraye

et al. 2008

American Journal of Surgical Pathology

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Distinction between ulcerative colitis (UC) and Crohn disease (CD) in mucosal biopsies is often difficult. Das-1 and CG-3 are monoclonal antibodies directed against an unknown colonic epithelial protein and human tropomyosin isoform-5, respectively, both show altered expression in patients with UC. In this study, we evaluated the utility of Das-1 and CG-3 in distinguishing UC from CD and from other types of colitis. One colonic biopsy specimen from each of 85 patients with confirmed UC (n=25), CD (n=15), lymphocytic (n=15), collagenous (n=15), and ischemic (n=15) colitis, and also 10 samples from normal controls, were stained for Das-1 and CG-3 using standard techniques. Reactivity for Das-1 and CG-3 was noted to be absent or present, and the location (ie, surface+/-crypt epithelium) and degree (weak or strong) of CG-3 staining was recorded. Loss of Das-1 staining occurred more frequently in UC (96%) compared with CD (20%), lymphocytic (20%), collagenous (13%), and ischemic colitis (0%) cases, as well as controls (10%, P<0.001 for all comparisons). CG-3 positivity in crypt epithelium was significantly more common in UC (52%) compared with the other groups (P< or =0.02 for all comparisons). The combination of strong crypt CG-3 staining and loss of Das-1 staining was noted in 44% of UC cases, but not in any other type of colitis (P=0.003 for all comparisons). We conclude that the patterns of Das-1 and CG-3 staining in colonic mucosal biopsies may be clinically useful in distinguishing UC from CD and from other colitidies.

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“…It is externalized by the chaperone molecule colon epithelial protein in colonic epithelial cells, but not in small intestinal cells. Mirza et al suggested that this organ-specific antigenic display may play a role as a local trigger of the immune response and as a factor that perpetuates colon-restricted disease in UC [73]. Furthermore, antibodies against tropomyosin were shown to have a cytotoxic activity on hTM5-expressing cells.…”

Section: Antitropomyosin Antibodiesmentioning

confidence: 96%