Autoimmunity to tropomyosin isoforms in ulcerative colitis (UC) patients and unaffected relatives

Biancone, Livia; Monteleone, Giovanni; Marasco, R.; Pallone, Francesco

doi:10.1046/j.1365-2249.1998.00610.x

Cited by 42 publications

(30 citation statements)

References 36 publications

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“…21 Antibodies to human tropomyosin isoform 5 were found in 19% of CD patients, in 64% of UC patients, and in 4% of healthy controls. 22 Antibodies to complement C3 were found in 23% of IBD patients, but not in healthy controls. 23 Antibodies to pancreatic tissue were found in 39% of CD patients, 4% of UC patients, but not in healthy controls or patients with celiac disease.…”

Section: Discussionmentioning

confidence: 98%

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Identification of a novel autoantigen in inflammatory bowel disease by protein microarray

Vermeulen

Beéck

Vermeire

et al. 2011

Inflammatory Bowel Diseases

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Section: Discussionmentioning

confidence: 98%

“…Antibodies to these antigens have been described in CD, UC, and IBD, respectively. [21][22][23] To our knowledge, antibodies to FAM84A have not been described previously. FAM84A is a gene whose expression has been reported to be upregulated in colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel autoantigen in inflammatory bowel disease by protein microarray

Vermeulen

Beéck

Vermeire

et al. 2011

Inflammatory Bowel Diseases

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“…In the cell membrane hTM5 is found complexed with a 200 kDa colonic epithelial protein (CEP), and this complex is speculated to serve as the true target for the Das-1 antibody [27] . Antibodies against hTM5 have been detected in UC patients without PSC [28] , and antihTM5 in UC sera has recently been shown to induce cytotoxicity against colonic epithelial cells in vitro [29] . In PSC patients without concomitant UC, a single study identified antibodies against a 9-amino acid sequence from hTM (not isoform specific) in 100% (8/8) of patients as compared with 69% (33/48) of UC patients and 0/6 PBC patients [30] .…”

Section: Antibodies Against Biliary and Colonic Epithelial Antigensmentioning

confidence: 99%

Autoantibodies in primary sclerosing cholangitis

Hov¹,

Boberg²,

Karlsen³

2008

WJG

150

100

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The aetiology of primary sclerosing cholangitis (PSC) is not known and controversy exists as to whether PSC should be denominated an autoimmune disease. A large number of autoantibodies have been detected in PSC patients, but the specificity of these antibodies is generally low, and the frequencies vary largely between different studies. The presence of autoantibodies in PSC may be the result of a nonspecific dysregulation of the immune system, but the literature in PSC points to the possible presence of specific antibody targets in the biliary epithelium and in neutrophil granulocytes. The present review aims to give an overview of the studies of autoantibodies in PSC, with a particular emphasis on the prevalence, clinical relevance and possible pathogenetic importance of each individual marker.

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“…Autoimmune mechanisms may play an important role in the pathogenesis of this disease. Various kinds of autoantibodies, such as anti-neutrophil cytoplasmic antibodies [1][2][3][4] and antitropomyosin antibodies, [5][6][7] have been demonstrated in serum samples of patients with ulcerative colitis.…”

Section: Introductionmentioning

confidence: 99%

Circulating autoantibodies against purified colonic mucin in ulcerative colitis

et al. 2000

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To clarify the role of colonic mucin in the autoimmune process of ulcerative colitis, circulating antibodies against human colonic mucin were investigated. Purified colonic mucin, obtained from human colonic mucosa by gel filtration, using a Bio-Gel A-1.5-m column and CsCl equilibrium density gradient, was divided into soluble mucin (S-mucin) secreted extracellularly and membranous mucin (M-mucin) binding to cell membrane. Sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting analysis showed that antibodies in the serum samples of some patients with ulcerative colitis recognized purified S- and M-mucin of >180-kD. By enzyme-linked immunosorbent assay (ELISA), anti-mucin antibodies were detected in 11 of 60 patients with ulcerative colitis (18%). In contrast, the antibodies were not detected in 22 patients with Crohn's disease. The titers of antimucin antibodies against S-mucin and M-mucin were not different in each patient. By ELISA using mucin in which the sugar chains were destroyed by neuraminidase or NaIO4 treatment, it was demonstrated that anti-mucin antibodies recognized the epitopes of either the sugar chain or the core protein exposed through destruction of the sugar chains. We then investigated the relationship between anti-mucin antibodies and the patients' clinical features. Anti-mucin antibodies were detected in 6 of 15 patients with chronic continuous type ulcerative colitis (40%) and in 5 of 26 patients with relapsing-remitting type (19%), but there was no antimucin antibody-positive serum in patients who had had only one attack without any relapse. These results suggest that anti-mucin antibodies could be a disease marker for ulcerative colitis and that immunological abnormalities in colonic mucin contribute to the persistence of colonic mucosal inflammation.

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Autoimmunity to tropomyosin isoforms in ulcerative colitis (UC) patients and unaffected relatives

Cited by 42 publications

References 36 publications

Identification of a novel autoantigen in inflammatory bowel disease by protein microarray

Identification of a novel autoantigen in inflammatory bowel disease by protein microarray

Autoantibodies in primary sclerosing cholangitis

Circulating autoantibodies against purified colonic mucin in ulcerative colitis

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