Autoinduction of tumor necrosis factor-α in FRTL-5 rat thyroid cells

Abstract

Tumor necrosis factor-(TNF ) may play a role in the development of autoimmune thyroiditis such as Hashimoto's thyroiditis. In the present study, we examined whether TNF induced its own expression in FRTL-5 rat thyroid cells. Lipopolysaccharide (LPS) markedly increased TNF mRNA levels in FRTL-5 cells as assessed by semiquantitative RT-PCR. In addition, LPSstimulated cells released TNF protein into the culture medium. Similarly, TNF induced its own gene and protein expression in FRTL-5 cells as assessed by RT-PCR and metabolic labeling and immunoprecipitation of TNF . The autoinduction of TNF gene was also observed in TNF -stimulated human thyroid epithelial cells. TNF induction was specific to LPS and TNF since interferon-or amiodarone failed to increase TNF mRNA levels in FRTL-5 cells. Human TNF induced rat TNF gene expression, indicating that type 1 TNF receptor (TNF-R) is involved in the autoinduction. TNF did not increase either type 1 or type 2 TNF-R mRNA levels, suggesting that upregulation of TNF receptors is not involved in the autoinduction of TNF . Although the biological significance of autoinduction of TNF remains unclear, our results suggest that thyroid epithelial cells may participate in the development of autoimmune thyroiditis through production of TNF . Furthermore, inhibition of TNF production in the thyroid may represent a novel approach to mitigating inflammation in autoimmune thyroiditis.

MYMD-1, a Novel Immunometabolic Regulator, Ameliorates Autoimmune Thyroiditis via Suppression of Th1 Responses and TNF-α Release

“…No effect was seen for follicular B cell populations residing in spleen and lymph nodes. ability to release TNF-a when exposed to inflammatory contexts (39). We found that MYMD-1 decreased both the ability of CD4 T cells to release TNF-a in vitro and the serum levels of TNF-a, in keeping with the known ability of alkaloids to inhibit TNF-a production.…”

Efficacy and safety of Ophiocordyceps sinensis in the treatment of Hashimoto’s thyroiditis: a systematic review and meta-analysis

“…in thyroid follicular cells enhance the inflammatory response through the production of nitric oxide and prostaglandins ( Khan et al, 2015 ). Mori et al (2005) observed the autoinduction of TNF-α in rat thyroid cells. Additionally, in human thyroid cells, TNF-α has a promoting effect on the expression of several inflammasome components ( Guo et al, 2018 ).…”