Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis

Michálek, Jaroslav; Moster, R; Lukác, L; Proefrock, Kenneth; Petrasovic, M; Rybar, Jakub; Capkova, Martina; Chaloupka, Ales; Darinskas, Adas; Křístek, Jan; Travnik, Jan; Jabandžiev, Petr; Cibulka, Marek; Holek, Michal; Jurík, Michal; Skopalík, Josef; Křístková, Zlatuše; Dudasova, Zuzana

doi:10.3727/096368915x686760

Cited by 34 publications

(30 citation statements)

References 32 publications

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“…Indeed, to date, clinical studies suggest that SVF and ATD-MSCs are safe and capable of tissue repairing [15, 18, 19, 29, 30]. Various well-defined harvesting techniques are available for liposuction procedure [27, 31, 32], even though there is not one method providing the best results in terms of viability and volume of adipose tissue with its cellular components.…”

Section: Discussionmentioning

confidence: 99%

“…The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion [15–19]; the efficacy of multiple infusions of ATD-MSCs in the articular joint is considered but still debated due to lack of data from clinical trials [19]. The availability of banking adipose tissue would allow treating with multiple infusions patients, avoiding repeated liposuction procedures for patients.…”

Section: Introductionmentioning

confidence: 99%

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Adipose Derived‐Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Roato

Alotto²,

Belisario

et al. 2016

Stem Cells International

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Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious. Here we studied some crucial aspects of adipose tissue banking procedures, evaluating ATD-MSCs viability, and differentiation capability after cryopreservation, to guarantee the quality of the tissue for multiple infusions. We reported that the presence of local anesthetic during lipoaspiration negatively affects cell viability of cryopreserved adipose tissue and cell growth of ATD-MSCs in culture. We observed that DMSO guarantees a faster growth of ATD-MSCs in culture than trehalose. At last, ATD-MSCs derived from fresh and cryopreserved samples at −80°C and −196°C showed viability and differentiation ability comparable to fresh samples. These data indicate that cryopreservation of adipose tissue at −80°C and −196°C is equivalent and preserves the content of ATD-MSCs in Stromal Vascular Fraction (SVF), guaranteeing the differentiation ability of ATD-MSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adipose Derived‐Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Roato

Alotto²,

Belisario

et al. 2016

Stem Cells International

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“…19 A total of 1856 joints mainly knee and hip joints were treated with a single dose of SVF cells and a follow up after treatment was done at 3, 6 and 12 months and found that 75% score improvement was found in 63% of the patients and at least 50% score improvement was noted in 91% of the patients after 12 months of after the therapy. 20 Adipose derived stromal vascular fraction (SVF), containing large number of cells. Adipose tissue-derived MSCs are more genetically stable in a long term culture, display a lower senescence ratio and higher proliferative capacity.…”

Section: Introductionmentioning

confidence: 99%

Autologous grafting of non manupulated freshly isolated - adipose tissue derived stromal vascular fraction in single surgical sitting for treatment of knee osteoarthritis

et al. 2016

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Background: Osteoarthritis (OA) is one of the major cause of morbidity, having a substantial influence on health quality of life, imposing enormous burden of cost on the health care system. It is a chronic degenerative disorder that is characterised by articular cartilage degeneration. It can be caused by aging, heredity and injury from trauma or disease. Primary symptoms of OA include joint pain, stiffness and limitation of movement. Disease progression is usually slow but can ultimately lead to joint failure with pain and disability. Stromal vascular fraction (SVF) derived from adipose tissue is a rich source of pre adipocytes, mesenchymal cells (MSC), endothelial progenitor cell, T cells, B cells, mast cells as well as adipose tissue macrophages. It can be easily obtained from loose connective tissue that is associated with adipose tissue by lipoaspiration under local anaesthesia. SVF is isolated without using any enzymes or chemicals and its autologous grafting is done in a single surgical sitting. Here, we evaluated safety and clinical efficacy of freshly isolated Autologous SVF cells in patients with grade 2-4 degenerative osteoarthritis (OA). . Methods: A total of 116 joints mainly knee OA were treated with autologous grafting of SVF done in a single surgical sitting. A total of 116 joints studied out of which 80 joints were followed up for 12 months, 88 joints followed for 9 months, 110 joints followed for 6 months and finally all 116 joints were followed for minimum 1 month for safety and efficacy. Results: Modified KOOS clinical score was used to evaluate clinical effect and was based on pain, non-steroid analgesic usage, limping, extent of joint movement, and stiffness evaluation before and at pre-operative, 1 Month post-op, 6 months post-op and 12 Months post-op after grafting. No side effects, systemic infection or cancer was associated with Autologous grafting of SVF. There was a significant improvement from pre-op to post op in all the followed patients. Average KOOS score improved from pre-operative 46.4 to post-operative 12 months average 77.9 i.e. very significant improvement in all grades. All sub-scale parameter for pain, symptoms, activity of living and quality of life showed significant improvement. Higher grade of OA were associated with comparatively slower healing. Conclusions: Autologous grafting of SVF in single surgical sitting is a novel and promising treatment approach for patients with degenerative OA. This treatment method was found to be minimal invasive, safe and cost-effective treatment modality for osteoarthritis.

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“…Multi-center case control study [10] Currently, there is no cure for painful OA in stages 2 and 3. For these patients, the intra-articular injection of ASCs can be an alternative treatment.…”

Section: Outcome and Commentsmentioning

confidence: 99%

“…Also, obtaining approximately 100 g of adipose tissue and percutaneous joint injections of ASCs with or without PRP are considered to be a minimally invasive procedure and can be readily accepted by patients [6]. These procedures carry a relatively low rate of morbidity and side effects [7][8][9][10].…”

Section: Outcome and Commentsmentioning

confidence: 99%

A Promising New Treatment for Osteoarthritis

Pak¹,

Lee²,

Lee³

2016

Transl Biomed

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At present, there is no definite cure for the underlying causes of osteoarthritis (OA). However, adipose tissuederived stem cells (ASCs) in the form of stromal vascular fraction can offer an alternative at this time. ASCs are one type of mesenchymal stem cells that have been utilized and have demonstrated an ability to regenerate cartilage in human patients with OA. Here, we highlight the possibility of ASCs regenerating cartilage in human OA patients.

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Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis

Abstract: Ahead of Print article withdrawn by publisher

Cited by 34 publications

References 32 publications

Adipose Derived‐Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Adipose Derived‐Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Autologous grafting of non manupulated freshly isolated - adipose tissue derived stromal vascular fraction in single surgical sitting for treatment of knee osteoarthritis

A Promising New Treatment for Osteoarthritis

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