Autologous fibrin-coated small-caliber vascular prostheses improve antithrombogenicity by reducing immunologic response

Hasegawa, Tomomi; Okada, Kenji; Takano, Yoshihito; Hiraishi, Yoshiaki; Okita, Yutaka

doi:10.1016/j.jtcvs.2006.12.049

Cited by 16 publications

(9 citation statements)

References 27 publications

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“…In addition, innumerable in vitro studies have focused on the mechanisms of fibrinogen adsorption in an attempt to understand its thrombogenic potential [(4–10) and references therein]. Paradoxically, other studies have focused on the design of anti-thrombogenic vascular grafts utilizing fibrin(ogen)-coated surfaces with apparently beneficial results in animal studies and in humans (11–14). …”

Section: Introductionmentioning

confidence: 99%

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Сафиуллин

Christenson

Owaynat³

et al. 2015

Biomaterials

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Adsorption of fibrinogen on the luminal surface of biomaterials is a critical early event during the interaction of blood with implanted vascular graft prostheses which determines their thrombogenicity. We have recently identified a nanoscale process by which fibrinogen modifies the adhesive properties of various surfaces for platelets and leukocytes. In particular, adsorption of fibrinogen at low density promotes cell adhesion while its adsorption at high density results in the formation of an extensible multilayer matrix, which dramatically reduces cell adhesion. It remains unknown whether deposition of fibrinogen on the surface of vascular graft materials produces this anti-adhesive effect. Using atomic force spectroscopy, single cell force spectroscopy, and standard adhesion assays with platelets and leukocytes, we have characterized the adhesive and physical properties of the contemporary biomaterials, before and after coating with fibrinogen. We found that uncoated PET, PTFE and ePTFE exhibited high adhesion forces developed between the AFM tip or cells and the surfaces. Adsorption of fibrinogen at the increasing concentrations progressively reduced adhesion forces, and at ≥2 µg/ml all surfaces were virtually nonadhesive. Standard adhesion assays performed with platelets and leukocytes confirmed this dependence. These results provide a better understanding of the molecular events underlying thrombogenicity of vascular grafts.

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Section: Introductionmentioning

confidence: 99%

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Сафиуллин

Christenson

Owaynat³

et al. 2015

Biomaterials

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“…Because fibrinogen and thrombin, the source of fibrin, can be obtained from the peripheral blood of any individual [14], fibrin gel is one of the ideal biomaterials for tissue engineering purpose when applied to one’s self. Bone marrow derived mesenchymal stem cells (BMSCs) can differentiate into osteoblasts and secret a number of bone-related matrix proteins when they are cultured with a special osteogenic medium [15].…”

Section: Introductionmentioning

confidence: 99%

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material

et al. 2011

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Biomaterials containing components similar to the native biological tissue would have benefits as an implantable scaffold material. To obtain such biomimetic materials, cells may be great contributors because of their crucial roles in synthetic organics. In addition, the synthesized organics—especially those derived from osteogenic differentiated cells—become a place where mineral crystals nucleate and grow even in vitro. Therefore, to fabricate an organic/inorganic composite material, which is similar to the biological osteoid tissue, bone marrow derived mesenchymal stem cells (BMSCs) were cultured in a 3D fibrin gel in this study. BMSCs secreted bone-related proteins that enhanced the biomineralization within the gel when the cells were cultured with an osteogenic differentiation medium. The compositions of both synthesized matrices and precipitated minerals in the obtained materials altered depending on the cell culture period. The mineral obtained in the 3D gel showed low crystalline hydroxyapatite. The composite materials also showed excellent osteoconductivity with new bone formation when implanted in mice tibiae. Thus, we demonstrated the contributions of cells for fabricating implantable organic/inorganic composite gel materials and a method for controlling the material composition in the gel. This cell-based material fabrication method would be a novel method to fabricate organic/inorganic composite biomimetic materials for bone tissue engineering.

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“…Fibrinogen and fibrin are the principal components of thrombi formed at sites of vascular injury. Furthermore, the presence of fibrin(ogen) on implanted vascular grafts is the major factor of their biocompatibility (8). The fibrin(ogen) substrates support attachment of platelets and leukocytes via integrins α IIb β 3 and α M β 2 , respectively.…”

mentioning

confidence: 99%

Control of Integrin α_IIbβ₃ Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

Podolnikova¹,

Yermolenko²,

Fuhrmann³

et al. 2009

Biochemistry

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The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in an aggregated multilayered material characterized by low adhesion forces. In contrast, immobilization of fibrinogen at low density produced a single layer in which molecules were directly attached to the solid surface, resulting in higher adhesion forces. Consistent with their distinct physical properties, low- but not high-density fibrinogen induced strong αIIbβ3-mediated outside-in signaling in platelets, resulting in their spreading. Moreover, while intact fibrin gels induced strong signaling in platelets, deposition of fibrinogen on the surface of fibrin resulted in diminished cell signaling. The data suggest that deposition of a multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties of surfaces, which results in reduced force generation and insufficient signaling. The mechanism whereby circulating fibrinogen alters adhesive properties of fibrin clots may have important implications for control of thrombus formation and thrombogenicity of biomaterials.

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Autologous fibrin-coated small-caliber vascular prostheses improve antithrombogenicity by reducing immunologic response

Abstract: These findings suggest that autologous fibrin coating in thrombin-free fibrin-coated vascular prostheses improve antithrombogenicity by reducing immunologic response and have a potential for clinical use in hybrid small-caliber vascular grafts.

Cited by 16 publications

References 27 publications

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material

Control of Integrin α_IIbβ₃ Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

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Autologous fibrin-coated small-caliber vascular prostheses improve antithrombogenicity by reducing immunologic response

Abstract: These findings suggest that autologous fibrin coating in thrombin-free fibrin-coated vascular prostheses improve antithrombogenicity by reducing immunologic response and have a potential for clinical use in hybrid small-caliber vascular grafts.

Cited by 16 publications

References 27 publications

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating

Cell-Based Fabrication of Organic/Inorganic Composite Gel Material

Control of Integrin αIIbβ3 Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates

Contact Info

Product

Resources

About

Control of Integrin α_IIbβ₃ Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates