Autologous, Noncultured Epidermal Cell Suspension Grafting in the Management of Mechanically and Chemically Induced Leukodermic Scars

Vyas, Nikki S.; Lawrence, Kelsey; Griffith, James L.; Hamzavi, Iltefat

doi:10.1177/1203475415581311

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Another RCT assessed the approach of combination split‐thickness autografts with harvested skin cell suspension and the split‐thickness autograft alone for chronic wound treatment, which demonstrated that complementary split‐thickness autologous skin grafting with autologous skin cells harvested using ReCell technology improved the healing rate of chronic wounds . Meanwhile, ReCell technology in combination with other technologies, such as lipofilling subdermal reconstruction, CO 2 laser ablation, surgical excision, or medical needling appeared to be effective methods to correct injured sequelae including hypopigmentation and scar in skin cancer or burns even in congenital melanocytic nevi …”

Section: Resultsmentioning

confidence: 99%

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

Ren

Liu

et al. 2019

J of Cosmetic Dermatology

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Background Regenerative Epithelial Suspension can lead to the restoration of wound and repigmentation, which can be gained by ReCell medical device to treat scar and depigmentation diseases. Objectives To report the effectivity of ReCell combined with microdermabrasion in scar and depigmentation diseases and review the literature of this new technology. Methods We gave a differential donor⁄recipient ratio of about 1:20‐30 with vitiligo, 1:40 with postburn construction, 1:80 with acne scar, and 1:120 with adult congenital melanocytic nevus, 1:80 with pediatrics, respectively. Photographs of patients before treatment and 3 months following the last treatment session were used to evaluate the effectivity. Results A total of 8 patients including vitiligo vulgaris, postburn reconstruction, acne scars, and congenital melanocytic nevi treated by ReCell technology combined with microdermabrasion showed significant improvement in skin texture and color. And 17 studies of the research on ReCell technology were totally included in the systematic review. Conclusion Our investigation showed that Regenerative Epithelial Suspension gained by ReCell technology combined with microdermabrasion may improve scar and depigmentation diseases.

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Section: Resultsmentioning

confidence: 99%

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

Ren

Liu

et al. 2019

J of Cosmetic Dermatology

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“…In a recent literature review, Madu et al 2 . list many treatments for postinflammatory hypopigmentation, including the laser hole technique using nonfractional CO 2 laser, 35 fractional CO 2 laser, 1,36 fractional CO 2 laser with topical latanoprost, 1,36 1,550‐nm fractional laser with or without topical bimatoprost 36 or with topical tretinoin or pimecrolimus, 37 skin grafting, 36,38–40 needling and noncultured autologous skin cell transplantation, 41 needling and UVA, 42 excimer laser, 43–45 dermabrasion, 39 cosmetic camouflage, 46 and cosmetic tattooing 47 . Laser treatments 37 and microneedling 41 can induce repigmentation by collagen remodeling 37,48 .…”

Section: Discussionmentioning

confidence: 99%

Is it possible to repigment iatrogenic and traumatic hypochromic lesions? A case series using the MMP^® drug delivery technique with 5‐fluorouracil and bleomycin

Arbache

Arbache²,

Hirata

2021

Int J Dermatology

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Objectives We present the results of 11 patients with hypochromic lesions treated with antifibrotic agents delivered through the MMP® technique. Methods Eleven patients with hypochromic lesions because of external injuries were treated at a single clinic with 5‐fluorouracil (5‐FU) and/or bleomycin using the MMP® drug delivery technique. Treatment sessions were repeated at 30‐day intervals until patient satisfaction. The primary outcome was repigmentation of the lesions, assessed independently by two dermatologists and by the patient. Results The MMP® technique injected 0.048 mg/cm2 of 5‐FU or 0.0028 U/cm2 of bleomycin. The accumulated 5‐FU and bleomycin density per patient ranged from 0.02 to 0.77 mg and 0.0022 to 0.2800 U/ml, respectively. Patients were treated with 1 to 6 MMP® sessions with 5‐FU (3 patients), bleomycin (6 patients), or both drugs (2 patients). After the last session, all patients had a significant improvement (>75%) of the lesions compared to baseline. There were no procedure‐related short‐ or long‐term adverse effects in any of the participants up to their last follow‐up visit. Conclusions The injection of antifibrotic agents using the MMP® technique was effective and safe in the treatment of hypochromic lesions. This can be a new therapeutic option for these lesions.

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“…The amount, type, and distribution of the dark biopolymer melanin in the skin contribute to the uniqueness of every person [1,2]. Already after deep-partial thickness-burn trauma, therapy results can include postburn hypopigmentation (leucoderma) [3][4][5]. Visual impressions of body form and skin color are important factors for interactions among humans, especially for facial appearance.…”

Section: Introductionmentioning

confidence: 99%

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

Johnen¹,

Hartmann²,

Plettig³

et al. 2019

SCR

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A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

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Autologous, Noncultured Epidermal Cell Suspension Grafting in the Management of Mechanically and Chemically Induced Leukodermic Scars

Abstract: In this small case series, lack of fluorescence in leukodermic scars may be a useful negative prognostic indicator for MKTP, but additional trials are needed to verify that this is not due to melanocompetency.

Cited by 7 publications

References 25 publications

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

Is it possible to repigment iatrogenic and traumatic hypochromic lesions? A case series using the MMP^® drug delivery technique with 5‐fluorouracil and bleomycin

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

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Autologous, Noncultured Epidermal Cell Suspension Grafting in the Management of Mechanically and Chemically Induced Leukodermic Scars

Abstract: In this small case series, lack of fluorescence in leukodermic scars may be a useful negative prognostic indicator for MKTP, but additional trials are needed to verify that this is not due to melanocompetency.

Cited by 7 publications

References 25 publications

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

The use of noncultured regenerative epithelial suspension for improving skin color and scars: A report of 8 cases and review of the literature

Is it possible to repigment iatrogenic and traumatic hypochromic lesions? A case series using the MMP® drug delivery technique with 5‐fluorouracil and bleomycin

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

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Resources

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Is it possible to repigment iatrogenic and traumatic hypochromic lesions? A case series using the MMP^® drug delivery technique with 5‐fluorouracil and bleomycin