Autologous rabbit adipose tissue-derived mesenchymal stromal cells for the treatment of bone injuries with distraction osteogenesis

Sunay, Özgür; Can, Geylani; Çakır, Zeynep; Denek, Ziya; Kozanoğlu, İlknur; Erbil, Güven; Yılmaz, Mustafa; Baran, Yusuf

doi:10.1016/j.jcyt.2013.02.004

Cited by 37 publications

(39 citation statements)

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“…Those cells have been shown to reside in a perivascular location, and ADSC may in fact be vascular stem cells (VSC) (Lin et al 2010). This statement is very plausible and explains why the multipotent cells isolated from subcutaneous fat depot can give rise to several cell lineages such as adipocytes, osteoblasts, and chondrocytes; why they are abundant; and why they show better multifunctional plasticity and are identified by numerous authors as a prospective cell source for autologous transplantation in regenerative medicine (Tran and Kahn 2010;Sunay et al 2013).…”

Section: First Induction Cycle Second Induction Cycle Third Inductionmentioning

confidence: 99%

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Vachkova

Bosnakovski

Yonkova

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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Rabbits are considered as appropriate animal models to study some obesity-associated abnormalities because of the similarity of their blood lipid profile and metabolism to humans. The current study was focused on comparison of adipose differentiation ability in rabbit adipose-derived stem cells (ADSC) in vitro. Subcutaneous and visceral stromal vascular fractions (SVF) were isolated from three 28-d-old New Zealand rabbits by collagenase digestion. Supernatants from both isolates were collected 24 h after the initial plating. On the fourth passage, all isolated cell types undergo triplicate adipogenic induction. The adipose induction potential was calculated as percentage of increasing optical density (OD) values. The data revealed that with increasing the number of induction cycles, the induction tendency in visceral ADSC decreased in contrast to the subcutaneous ones. Although the supernatants did not reach induction levels of their relevant precursors, they follow the same pattern in both subcutaneous and visceral ADSC. All cell types successfully passed osteogenic and chondrogenic differentiation. In conclusion, the best adipose induction ability was observed in directly plated subcutaneous cell population. The increase of induction numbers depressed adipose induction ability in cell populations derived from visceral fat depots.

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Section: First Induction Cycle Second Induction Cycle Third Inductionmentioning

confidence: 99%

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Vachkova

Bosnakovski

Yonkova

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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“…Cultured BMSCs were observed under the microscope to control their expansion and morphology. When primary cultures became 80-90 % confluent, cells were detached from culture flask surface using Trypsin/EDTA solution and cells were seeded onto fresh plates [20].…”

Section: Isolation and Cultivation Of Mesenchymal Stem Cells From Moumentioning

confidence: 99%

“…The GFP-transfected cells were collected from culture flask via trypsinization and counted upon trypan blue staining. BMSCs were suspended in normal saline to introduce them into allergic rhinitis animal models [20].…”

Section: Transfection Of Mscs With Gfpmentioning

confidence: 99%

Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model

Karaman

Adan

Kıray

et al. 2016

Eur Arch Otorhinolaryngol

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Previous studies showed that bone marrow-derived mesenchymal stem cells (BMSCs) could ameliorate a variety of immune-mediated and inflammatory diseases due to their immunomodulatory and anti-inflammatory effects. In this study, we developed a mouse model of ovalbumin (OVA) induced allergic inflammation in the upper airways and evaluated the effects of the intraperitoneal administration of BMSCs on allergic inflammation. Twenty-five BALB/c mice were divided into five groups; group I (control group), group II (sensitized and challenged with OVA and treated with saline-placebo group), group III (sensitized and challenged with OVA and treated with 1 × 10 BMSCs), group IV (sensitized and challenged with OVA and treated with 2 × 10 BMSCs), and group V (sensitized and challenged with phosphate buffered saline (PBS) and treated with 1 × 10 BMSCs). Histopathological features (number of goblet cells, eosinophils and mast cells, basement membrane, epithelium thickness, and subepithelial smooth muscle thickness) of the upper and lower airways and BMSCs migration to nasal and lung tissue were evaluated using light and confocal microscopes. Levels of cytokines in the nasal lavage fluid and lung tissue supernatants were measured using enzyme-linked immunosorbent assay (ELISA). Confocal microscopic analysis showed that there was no significant amount of BMSCs in the nasal and lung tissues of group V. However, significant amount of BMSCs were observed in group III and IV. In OVA-induced AR groups (group II, III, and IV), histopathological findings of chronic asthma, such as elevated subepithelial smooth muscle thickness, epithelium thickness, and number of goblet and mast cells, were determined. Furthermore, the number of nasal goblet and eosinophil cells, histopathological findings of chronic asthma, and IL-4, IL-5, IL-13, and NO levels was significantly lower in both BMSCs-treated groups compared to the placebo group. Our findings indicated that histopathological findings of chronic asthma were also observed in mice upon AR induction. BMSCs migrated to the nasal and lung tissues following intraperitoneal delivery and ameliorated to the airway remodeling and airway inflammation both in the upper and lower airways via the inhibition of T helper (Th) 2 immune response in the murine model of AR.

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“…30,31 The application of MSCs has been shown to improve the quality and quantity of bone healing including fractures or DO. 3,32,33 …”

Section: Introductionmentioning

confidence: 99%

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

Yang

Lin

Wang

et al. 2017

Bone & Joint Research

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ObjectivesDistraction osteogenesis (DO) mobilises bone regenerative potential and avoids the complications of other treatments such as bone graft. The major disadvantage of DO is the length of time required for bone consolidation. Mesenchymal stem cells (MSCs) have been used to promote bone formation with some good results.MethodsWe hereby review the published literature on the use of MSCs in promoting bone consolidation during DO.ResultsStudies differed in animal type (mice, rabbit, dog, sheep), bone type (femur, tibia, skull), DO protocols and cell transplantation methods.ConclusionThe majority of studies reported that the transplantation of MSCs enhanced bone consolidation or formation in DO. Many questions relating to animal model, DO protocol and cell transplantation regime remain to be further investigated. Clinical trials are needed to test and confirm these findings from animal studies.Cite this article: Y. Yang, S. Lin, B. Wang, W. Gu, G. Li. Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis: A contemporary review of experimental studies. Bone Joint Res 2017;6:385–390. DOI: 10.1302/2046-3758.66.BJR-2017-0023.

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Autologous rabbit adipose tissue-derived mesenchymal stromal cells for the treatment of bone injuries with distraction osteogenesis

Cited by 37 publications

References 50 publications

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model

Stem cell therapy for enhancement of bone consolidation in distraction osteogenesis

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