Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use

Stute, Norbert; Holtz, Katja; Bubenheim, Michael; Lange, Claudia; Blake, Felix; Zander, Axel R.

doi:10.1016/j.exphem.2004.09.003

Cited by 247 publications

(205 citation statements)

References 72 publications

Supporting

Mentioning

184

Contrasting

Unclassified

Order By: Relevance

“…Many studies have been performed to try and substitute the animal serum with human autologous or allogeneic serum or with synthetic media, but have not usually produced satisfactory results. [13][14][15] The addition of the PL has been suggested previously, [16][17][18] but the exact determination of hMSC yield starting from a reduced number of nucleated cells to reach clinically relevant numbers has never been shown previously.…”

Section: Discussionmentioning

confidence: 99%

Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts

Capelli

Domenghini

Borleri

et al. 2007

Bone Marrow Transplant

150

124

View full text Add to dashboard Cite

We compared two protocols for the expansion of human mesenchymal stromal cells (hMSCs) starting from diagnostic samples of BM aspirates (2-5 ml) or using the remnants in the bag and filter at the end of the BM infusions. The protocols differed in the presence of either 10% fetal bovine serum (FBS) or 5% platelet lysate (PL). We obtained a significantly (P ¼ 0.02) better expansion with PL, obtaining a median 1010-fold compared to 198-fold with a selected batch of FBS and in fewer days (29.8 in PL versus 41.4 in FBS). Overall, we recovered a variable number from 54.8 Â 10 6 to 365 Â 10 6 hMSCs in PL versus a variable number from 2.7 Â 10 6 to 31 Â 10 6 in FBS. No difference could be found in terms of gross morphology, differentiation potential, surface markers and immunological properties (inhibition of allogeneic PHA response and mixed lymphocyte reaction) of cells expanded with PL or FBS. The preparations were found within the range of acceptability for all the quality control criteria. Due to the clinical grade nature of the PL and the reproducibility of separate preparations, we propose this method to obtain hMSCs even from minute amounts of BM cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts

Capelli

Domenghini

Borleri

et al. 2007

Bone Marrow Transplant

150

124

View full text Add to dashboard Cite

show abstract

“…Autologous serum represents another alternative to FCS for cell expansion. Studies have demonstrated the possibility to replace FCS for expansion of human mesenchymal stem cells (Stute et al 2004, Shahdadfar et al 2005. In human chondrocytes recent data have demonstrated higher proliferation rates of articular and auricular chondrocytes in autologous and allogenic human serum in comparison to FCS (Tallheden et al 2005, Yanaga et al 2006.…”

Section: Discussionmentioning

confidence: 99%

“…If the cells have been exposed to FCS during cultivation, the following transplantation may induce immune or local inflammatory responses (Selvaggi et al 1997, Heiskanen et al 2007. Therefore, the development of FCS-free culturing protocols certainly represents an advantage for future development of cellular therapies, and the use of serum-free media, allogenic, and autologous serum have being already investigated as potential alternatives for growing cells in human regenerative medicine (Stute et al 2004, Shahdadfar et al 2005, Tallheden et al 2005. In addition to serum-supplementation, chondrocyte proliferation rates in monolayer culture have been shown to depend on factors such as the cell-seeding density (Mandl et al 2004) or the age of the donor (Barbero et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Influence of culture conditions on expansion and re-differentiation of chondrocytes from horses of different ages

Werren¹,

Diaz-Romero²,

Brehm³

et al. 2008

PHK

View full text Add to dashboard Cite

SummaryTo evaluate the influence of types of sera and the donors age on proliferation and re-differentiation capacities of equine chondrocytes, cartilage tissue from 10 horses was obtained in the age range 2.5 to 19 years. Chondrocytes were isolated from the fetlock-joints and expanded in media containing different types of sera: fetal calf serum (FCS), fetal equine serum (FES), adult equine serum (AES) and autologous serum. Cells were expanded in monolayers at low and high seeding densities. Chondrocytes re-differentiation capacities were assessed in micromass pellet cultures after 4 weeks of incubation in chondrogenic medium supplemented with or without 5ng/ml TGFβ1. The neocartilagineous tissue formation was evaluated through proteoglycan and collagen type II deposition, and Berne score visual evaluation system. Results showed that the low seeding density allows for higher proliferation rates in FCS, FES and AES. In addition chondrocytes from younger horses proliferate faster independently of the serum type. Re-differentiation capacities are influenced by the animals age, type of serum during expansion, and the presence of TGFβ1 in pellet cultures. Cells from young horses appear to produce better neocartilage upon expansion in autologous serum, while cells from older horses re-differentiated better upon expansion in FCS. The expansion conditions of potential clinical relevance include the correct choice of sera according to the age of horse during monolayer culture for better subsequent re-differentiation and lower seeding densities allowing for smaller biopsy sampling and therefore less injury to the donor site.

show abstract

“…Human BMSCs have been successfully cultured and grown in AS-supplemented medium [3,12,15]. Most of these cases involved the use of a culture medium supplemented with 10% AS, with no significant differences reported in the morphology or proliferation capacity of BMSCs compared to culturing with media with 10 or 20% FBS [3,10,12,15].…”

mentioning

confidence: 99%

“…In the clinical application of BMSCs, the use of fetal bovine serum (FBS) during culturing has been called into question and many investigations into culture methods without FBS are underway [6,12]. One proposed solution is the use of a culture medium supplemented with autologous serum (AS).…”

mentioning

confidence: 99%

Influence of an Autologous Serum-Supplemented Medium on the Proliferation and Differentiation into Neurons of Canine Bone Marrow Stromal Cells

Edamura

Kitaoka

Nakano

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.

show abstract

Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use

Cited by 247 publications

References 72 publications

Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts

Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts

Influence of culture conditions on expansion and re-differentiation of chondrocytes from horses of different ages

Influence of an Autologous Serum-Supplemented Medium on the Proliferation and Differentiation into Neurons of Canine Bone Marrow Stromal Cells

Contact Info

Product

Resources

About