Autologous Skeletal-Myoblast-Sheet Transplantation Improved Porcine Cardiac Function Without Increasing Risk of Arrhythmia

Terajima, Yutaka; Shimizu, Tatsuya; Tsuruyama, Shinpei; Sekine, Hidekazu; Hagiwara, Nobuhisa; Okano, Teruo

doi:10.1016/s0735-1097(12)61059-6

Cited by 3 publications

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“…In addition, cell sheet‐transplantation provides stronger therapeutic effects than dissociated cell injection . The effectiveness of cell sheet‐therapy has already been shown in various animal models of damaged heart, so that clinical researches using autologous myoblast sheets are now underway for treating severe heart disease at three institutions, including Osaka University in Japan . The therapeutic effect is thought to be mainly due to the paracrine effect of factors, including VEGF produced from transplanted cell sheets .…”

Section: Discussionmentioning

confidence: 99%

Rapid fabrication system for three‐dimensional tissues using cell sheet engineering and centrifugation

Hasegawa

Haraguchi

Okano

2015

J Biomedical Materials Res

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Three-dimensional (3D) tissues can be reconstructed by cell sheet technology, and various clinical researches using these constructed tissues have already been initiated to regenerate damaged tissues. While 3D tissues can be easily fabricated by layering cell sheets, the attachment period for cell adhesion between a cell sheet and a culture dish, or double-layered cell sheets normally takes 20-30 min. This study proposed a more rapid fabrication system for bioengineered tissue using cell sheet technology and centrifugation. A C2C12 mouse myoblast sheet harvested from a temperature-responsive culture dish will attach tightly to a culture dish or another cell sheet at 37°C after a 20 min-incubation. However, the same cell sheet centrifuged (12-34 × g) for 3 min also attached tightly to a dish or another cell sheet at 37°C after only a 3 min-incubation. The manipulation time was reduced by approximately two-thirds by centrifugation. The rapid attachments were also cross-sectionally confirmed by optical coherence tomography. These rapidly constructed cell sheet-tissues using centrifugation showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor, like those prepared by the conventional method; indicating complete cell sheet-attachment without any cell damage. This new system will be a powerful tool in the fields of cell sheet-based tissue engineering and regenerative medicine, and accelerate the use of cell sheets in clinical applications.

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Section: Discussionmentioning

confidence: 99%

Rapid fabrication system for three‐dimensional tissues using cell sheet engineering and centrifugation

Hasegawa

Haraguchi

Okano

2015

J Biomedical Materials Res

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Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine

et al. 2013

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Cell sheet engineering, which allows tissue engineering to be realized without the use of biodegradable scaffolds as an original approach, using a temperature-responsive intelligent surface, has been applied in regenerative medicine for various tissues, and a number of clinical studies have been already performed for life-threatening diseases. By using the results and findings obtained from the initial clinical studies, additional investigative clinical studies in several tissues with cell sheet engineering are currently in preparation stage. For treating many patients effectively by cell sheet engineering, an automated system integrating cell culture, cell-sheet fabrication, and layering is essential, and the system should include an advanced three-dimensional suspension cell culture system and an in vitro bioreactor system to scale up the production of cultured cells and fabricate thicker vascularized tissues. In this paper, cell sheet engineering, its clinical application, and further the authors' challenge to develop innovative cell culture systems under newly legislated regulatory platform in Japan are summarized and discussed.

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Cell sheet engineering for cardiac repair and regeneration

Haraguchi

Shimizu

Matsuura

et al. 2014

Cardiac Regeneration and Repair

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Autologous Skeletal-Myoblast-Sheet Transplantation Improved Porcine Cardiac Function Without Increasing Risk of Arrhythmia

Cited by 3 publications

References 0 publications

Rapid fabrication system for three‐dimensional tissues using cell sheet engineering and centrifugation

Rapid fabrication system for three‐dimensional tissues using cell sheet engineering and centrifugation

Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine

Cell sheet engineering for cardiac repair and regeneration

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