Autologous transplant in the treatment of severe aplastic anemia – A case report

Balint, Bela; Stamatović, Dragana; Todorović, Milena; Elez, Marija; Vojvodić, Danilo; Pavlović, Mirjana; Cucuz-Jokic, Milica

doi:10.1016/j.transci.2011.07.008

Cited by 16 publications

(13 citation statements)

References 16 publications

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“…There is an increasing interest in evaluation of the responsibility of the CD34 + subsets for complete and longterm repopulation, as a marker of cell harvest quality 1,[10][11][12] . In our initial clinical study, as a possible and potentially more objective collection predictor, the CD34 + /CD90 + subset evaluation was also recommended 19 . Precisely, on the basis of examination of the immature SC antigens and 7-aminoactinomycin D (7-AAD) viability, we suggested that the CD34 + /CD33 -, CD34 + /CD38 -, CD34 + /DR -, CD34 + /CD90 + cells could be superior predictive factor over circulating the total CD34 + count for an optimized collection-timing and outcome of autologous SC-transplant 19 .…”

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

“…In our initial clinical study, as a possible and potentially more objective collection predictor, the CD34 + /CD90 + subset evaluation was also recommended 19 . Precisely, on the basis of examination of the immature SC antigens and 7-aminoactinomycin D (7-AAD) viability, we suggested that the CD34 + /CD33 -, CD34 + /CD38 -, CD34 + /DR -, CD34 + /CD90 + cells could be superior predictive factor over circulating the total CD34 + count for an optimized collection-timing and outcome of autologous SC-transplant 19 . Other authors also confirmed that engraftment and hematopoietic recovery are not necessarily associated with cell dose of the total CD34 + cells 10 .…”

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

“…Other authors also confirmed that engraftment and hematopoietic recovery are not necessarily associated with cell dose of the total CD34 + cells 10 . Thus, the CD34 + /CD90 + cells could be a useful quality marker for the AP 10,19 , especially for prediction of the Plt recovery (reduced hazard of the prolonged thrombocytopenia) 11 . Consequently, the CD34 + /CD90 + cells could be a practical predictive factor for complete and durable hematopoietic reconstitution 10,11 .…”

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

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Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Balint¹,

Stanojević

Todorović

et al. 2017

VOJNOSANIT PREGL

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Background/Aim. Stem cells (SCs) guarantee complete/long-term bone marrow (BM) repopulation after SCtransplants. The aim of the study was to evaluate absolute count of total SCs (determined by ISHAGE-sequential-gating protocol -SC ish ) and relative frequency of immature CD34 + /CD90 + (CD90 + SC ish ) subset in peripheral blood (PB) as predictive factors of mobilization and apheresis product (AP) quality. Methods. Mobilization included chemotherapy and granulocyte-growth-factor (G-CSF). Harvesting was performed by Spectra-Optia-IDL-system. The SCs ish were determined as a constitutional part of CD34 + cells in the "stemcell-region" using FC-500 flow-cytometer. In this study, the original ISHAGE-sequential-gating protocol was modified by introduction of anti-CD90-PE monoclonal-antibody into the analysis of CD90 expression on SC ish (CD90 + SC ish ). The results were presented as a percentage of SC ish per nucleatedcell count, absolute SC ish count in μL of the PB or the AP, percentage of the CD90 + SC ish expressed to SC ish and absolute CD90 + SC ish count in μL of the PB or the AP. Results. The absolute count of total SC ish and CD90 + SC ish was significantly higher (p = 0.0007 and p = 0.0266, respectively) in the AP than in the PB samples. The CD90 + SC ish /total SC ish indexes from PB were higher than indexes from the AP (p = 0.039). Key words: stem cells; hematopoietic stem cell transplantation; bone marrow; flow cytometry; multiple myeloma; antineoplastic combined chemotherapy protocols.Apstrakt Uvod/Cilj. Matične ćelije (MĆ) obezbeđuju kompletnu/dugotrajnu repopulaciju kostne srži (KS) posle trans-plantacije. Cilj ove studije bila je procena apsolutnog broja ukupnih MĆ (utvrđena protokolom "ISHAGE-sequentialgating" -Stem-Cell ish [SC ish ]) i relativne učestalosti primitivnih podipova CD34 + /CD90 + (CD90 + SC ish ) u perifernoj krvi (PK) Ključne reči: ćelije, matične; transplantacija hematopoeznih matičnih ćelija; kostna srž; citometrija, protočna; multipli mijelom; lečenje kombinovanjem antineoplastika, protokoli.

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Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

Section: Sem -Standard Error Of the Meanmentioning

confidence: 99%

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Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Balint¹,

Stanojević

Todorović

et al. 2017

VOJNOSANIT PREGL

Self Cite

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“…In practice, allogeneic transplants require both an effective conditioning regimen, as well as optimized mobilization and harvesting protocol to obtain adequate SC yield [10][11][12][13][14]. For autologous transplants, the use of an optimized cryopreservation procedure guarantees the best recovery of thawed cell [15][16][17][18][19][20][21].…”

Section: Sažetakmentioning

confidence: 99%

“…Nowadays, the typical number of apheresis required is not more than one to three, and for anticoagulation ACD-B or ACD-A (with 2.2% citrate concentration) are used [10][11][12][13][14] (Figure 2).…”

Section: Stem Cell Sources and Harvestingmentioning

confidence: 99%

Stem cell transplant: From cell harvesting to cryopreservation

Balint¹,

Todorović

Urošević

et al. 2017

Med pregl

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Stem cells could be defined as cells capable for self-renewal with high proliferative capacity and extensive potential to differentiate into blood cells or some somatic cell types - ?plasticity? due to ?trans-differentiation? - such as osteocytes, chondrocytes, hepatocytes, myocytes, cardiomyocytes and even endothelial cells. Recent increasing clinical use of various cell-mediated therapeutic approaches has resulted in amplified needs for both stem cells and operating procedures to get a minimized cell damages during collection, purification and cryopreservation. The aim of cell harvesting procedures is to obtain the best stem cells yield, high purity and good viability/clonogenicity. The goal of optimized cryoinvestigation protocols is to minimize cell injuries during the freeze/thaw process (cryoinjury). Despite the fact that different stem cells collection protocols and cell freezing practice are already in routine use, a lot of questions related to the optimal blood stem cells harvesting, purification and cryopreservation are still unresolved.

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Principles and Practice of Stem Cell Cryopreservation

Pavlović

Balint

2012

SpringerBriefs in Electrical and Computer Engineering

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Autologous transplant in the treatment of severe aplastic anemia – A case report

Cited by 16 publications

References 16 publications

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Relative frequency of immature CD34+/CD90+ subset in peripheral blood following mobilization correlates closely and inversely with the absolute count of harvested stem cells in multiple myeloma patients

Stem cell transplant: From cell harvesting to cryopreservation

Principles and Practice of Stem Cell Cryopreservation

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