Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt's lymphoma

Abstract

A patient with Burkitt's lymphoma in complete remission received myeloablative consolidation treatment with superfractionated total body irradation (1,320 rad) and cyclophosphamide (200 mg/kg) followed by autologous transplantation of previously harvested and cryopreserved blood-derived hemopoietic stem cells. Seven successive leukaphereses were performed to yield a total of 55.2 X 10(9) mononuclear cells (MNC) comprising 15.1 X 10(6) CFU-GM or 4.34 X 10(6) CFU-GEMM. Following autologous blood stem cell transplantation (ABSCT), reconstitution of all cell lines occurred very rapidly, ie, 1,000 leucocytes per microL were reached after nine days, 500 granulocytes and 50,000 platelets per microL after ten days. B cells reached normal values around day 35 post- transplantation. CFU-GM first appeared in the circulating blood exhibiting an enormous overshoot. Some days later CFU-GM also appeared in the marrow. The kinetics and pattern of hemopoietic reconstitution after myeloablative treatment and ABSCT provide clear evidence that blood-derived hemopoietic stem cells are capable of completely restoring hemopoietic function in man. A possible reconstitutive advantage of blood over marrow-derived stem cells is discussed.

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining

“…Collection of PBPCs can be performed without general anesthesia, engraftment is faster, and supportive care and costs are reduced. PBPCs are harvested by leukapheresis after mobilization with chemotherapy and/or granulocyte–colony‐stimulating factor (G‐CSF) or granulocyte‐macrophage–CSF alone 1‐3 …”

A cell‐kinetic model of CD34+ cell mobilization and harvest: development of a predictive algorithm for CD34+ cell yield in PBPC collections

“…Collection of PBPCs can be performed without general anesthesia, engraftment is faster, and supportive care and costs are reduced. PBPCs are harvested by leukapheresis after mobilization with chemotherapy and/or G–CSF or GM–CSF alone 1–5 …”

A prospective, randomized, sequential, crossover trial of large‐volume versus normal‐volume leukapheresis procedures: effect on progenitor cells and engraftment

“…Collection of PBPCs can be performed without general anesthesia, engraftment is faster, and supportive care and costs are reduced. PBPCs are harvested by leukapheresis after mobilization with chemotherapy and/or granulocyte–colony‐stimulating factor (G–CSF) or granulocyte‐macrophage–CSF (GM–CSF) 1–5 …”