Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model

Abstract

Objectives To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model. Methods Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration. Results Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina. Conclusions Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth.

Regeneration of the Pulp Tissue: Cell Homing versus Cell Transplantation Approach: A Systematic Review

“…The studies that directly compared cell homing and cell transplantation [ 14 , 15 , 16 ] could not find a significant difference in apical healing [ 14 , 15 , 16 ]. Overall, the healing capacity seems to be sufficient in both techniques, as most of the lesions healed after treatment [ 14 , 15 , 16 ].…”

“…The studies that directly compared cell homing and cell transplantation [ 14 , 15 , 16 ] could not find a significant difference in apical healing [ 14 , 15 , 16 ]. Overall, the healing capacity seems to be sufficient in both techniques, as most of the lesions healed after treatment [ 14 , 15 , 16 ]. It seems apparent that the experimental groups (cell homing, cell transplantation) result in more apical healing than the control groups (no treatment) [ 16 ].…”

Endodontia regenerativa: revisão integrativa

“…Vislumbrando, dessa maneira, a realização de um transplante autólogo da polpa, eliminando a necessidade de altos custos com manipulação e reprodução celular. Huang et al, 2019 investigaram o potencial de regeneração pulpar através de um transplante autólogo da polpa dentária de um dente decíduo em dentes permanentes necróticos imaturos de cães. O estudo concluiu que a implantação direta de tecido pulpar de dentes decíduos produz resultados clínicos, radiográficos e histológicos favoráveis.…”

“…Porém, a ascensão da nova técnica de autotransplante de polpa, seja ela decídua ou permanente, trouxe subsídios clínicos para diminuição do custo financeiro e operacional com o cultivo celular, eliminando esta etapa clínica da terapia regenerativa, além disso, apresentando protocolos aceitáveis para os pacientes em que nem mesmo extrações dentárias são necessárias. Além de seu alto potencial de aceitação pelos pacientes, a técnica de autotransplante trouxe excelentes resultados no que tange os parâmetros regenerativos do complexo dentino-pulpar (Cehreli et al, 2022;Feitosa et al, 2021;Huang et al, 2019).…”

Proteomic Profiling of the First Human Dental Pulp Mesenchymal Stem/Stromal Cells from Carbonic Anhydrase II Deficiency Osteopetrosis Patients

“…Interest in stem cell therapies and regenerative medicines has grown notably over the last decade as their tremendous potential for effective treatments in a variety of medical diseases is being realized. Studies have shown that SHED are an ideal non-controversial and easily accessible cell source for use in regenerative medicine [ 15 , 16 , 17 , 18 ] The tissue regenerative effect of mesenchymal stem/stromal cells can be accomplished by the replacement of tissues with defects by differentiated dental MSCs/SCs themselves and/or through the induction of the de novo regeneration capacity of endogenous stem cells via remodeling of the stem cell niche [ 19 ].…”