Autologous transplantation of porcine myogenic precursor cells in skeletal muscle

Holzer, Nicolas; Hogendoorn, Simone; Zurcher, Line; Garavaglia, Guido; Yang, Sheng; König, Stéphane; Laumonier, Thomas; Ménétrey, Jacques

doi:10.1016/j.nmd.2004.11.001

Cited by 34 publications

(32 citation statements)

References 31 publications

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“…As previously reported (7), CD90 ϩ cells were mainly found in the perimysial compartment. With respect to CD56, which is generally considered as a marker of myogenic cells, our results confirmed the presence of CD56 ϩ cells in a satellite cell position as reported previously (19). A small proportion of CD34 ϩ cells was also detected in a satellite cell position (4%).…”

Section: C734supporting

confidence: 91%

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Perruchot

Lefaucheur

Barreau

et al. 2013

American Journal of Physiology-Cell Physiology

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A better understanding of the control of body fat distribution and muscle development is of the upmost importance for both human and animal physiology. This requires a better knowledge of the features and physiology of adult stem cells in adipose tissue and skeletal muscle. Thus the objective of the current study was to determine the type and proportion of these cells in growing and adult pigs. The different cell subsets of stromal vascular cells isolated from these tissues were characterized by flow cytometry using cell surface markers (CD11b, CD14, CD31, CD34, CD45, CD56, and CD90). Adipose and muscle cells were predominantly positive for the CD34, CD56, and CD90 markers. The proportion of positive cells changed with age especially in intermuscular adipose tissue and skeletal muscle where the percentage of CD90+ cells markedly increased in adult animals. Further analysis using coimmunostaining indicates that eight populations with proportions ranging from 12 to 30% were identified in at least one tissue at 7 days of age, i.e., CD90+/CD34+, CD90+/CD34−, CD90+/CD56+, CD90+/CD56−, CD90−/CD56+, CD56+/CD34+, CD56+/CD34−, and CD56−/CD34+. Adipose tissues appeared to be a less heterogeneous tissue than skeletal muscle with two main populations (CD90+/CD34− and CD90+/CD56−) compared with five or more in muscle during the studied period. In culture, cells from adipose tissue and muscle differentiated into mature adipocytes in adipogenic medium. In myogenic conditions, only cells from muscle could form mature myofibers. Further studies are now needed to better understand the plasticity of those cell populations throughout life.

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Section: C734supporting

confidence: 91%

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Perruchot

Lefaucheur

Barreau

et al. 2013

American Journal of Physiology-Cell Physiology

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“…The fact that grafted myoblasts undergo considerable mortality within the first days after their intramuscular transplantation was inferred from the posttransplantation loss of the different markers used to quantify the myoblasts in mice [110,[147][148][149][150][151] and pigs [69]. Additionally, we reported morphological evidence of apoptosis (by immunodetection of active caspase 3) and necrosis (by histochemical detection of intracellular calcium deposition) among mouse myoblasts implanted into mice, during this early post-transplantation period [151].…”

Section: The Early Survival Of the Myogenic Cellsmentioning

confidence: 95%

“…So far, myoblasts were the only myogenic cells that were proved unequivocally to engraft in humans and produce myofibers expressing donor-derived dystrophin in DMD patients [10][11][12][13] (Figures 2 and 3). Unlike the other cells that will be described below, myoblasts have been studied by several different teams in a wide range of animal models such as mice [61], rats [62,63], dogs [64,65], monkeys [66], rabbits [67,68], and pigs [69].…”

Section: Satellite Cells and Myoblasts: The Mainstay Of Myofibermentioning

confidence: 99%

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Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

Skuk

2013

ISRN Transplantation

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Myopathies produce deficits in skeletal muscle function and, in some cases, progressive and irreversible loss of skeletal muscles. The transplantation of myogenic cells, that is, cells able to differentiate into myofibers, is an experimental strategy for the potential treatment of some of these diseases. The objectives pursued by the transplantation of these cells are essentially three: (a) the fusion with the patient's myofibers to obtain the expression of therapeutic proteins into them, (b) the neoformation of new functional myofibers in skeletal muscles that were too degenerated by the progressive degeneration, and (c) the formation of a new pool of healthy donor-derived satellite cells. Although the repertoire of myogenic cells appears to have expanded in recent years, myoblasts are the only cells that have been demonstrated to engraft in humans. The present work aims to make a comprehensive review of the subject, from its beginnings to recent advances, including the preclinical experience in different animal models and recent clinical findings.

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“…It has been demonstrated that basic fibroblast growth Human muscle precursor cell (hMPC) transplantation is a promising clinical strategy to repair severe muscle factor (bFGF) or insulin-like growth factor type I (IGF-I) modulated the differentiation or the proliferation of injuries (32), prevent cardiac insufficiency (53,56), and delay the problems of inherited myopathies (50). Briefly, MPCs (2).…”

Section: Introductionmentioning

confidence: 99%

1,25-Dihydroxyvitamin D₃Increases the Transplantation Success of Human Muscle Precursor Cells in SCID Mice

et al. 2007

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Human muscle precursor cell (hMPC) transplantation is a potential therapy for severe muscle trauma or myopathies. Some previous studies demonstrated that 1,25-dihydroxyvitamin-D 3 (1,25-D 3 ) acted directly on myoblasts, regulating their proliferation and fusion. 1,25-D 3 is also involved in apoptosis modulation of other cell types and may thus contribute to protect the transplanted hMPCs. We have therefore investigated whether 1,25-D 3 could improve the hMPC graft success. The 1,25-D 3 effects on hMPC proliferation, fusion, and survival were initially monitored in vitro. hMPCs were also grafted in the tibialis anterior of SCID mice treated or not with 1,25-D 3 to determine its in vivo effect. Graft success, proliferation, and viability of transplanted hMPCs were evaluated. 1,25-D 3 enhanced proliferation and fusion of hMPCs in vitro and in vivo. However, 1,25-D 3 did not protect hMPCs from various proapoptotic factors (in vitro) or during the early posttransplantation period. 1,25-D 3 enhanced hMPC graft success because the number of muscle fibers expressing human dystrophin was significantly increased in the TA sections of 1,25-D 3 -treated mice (166.75 ± 20.64) compared to the control mice (97.5 ± 16.58). This result could be partly attributed to the improvement of the proliferation and differentiation of hMPCs in the presence of 1,25-D 3 . Thus, 1,25-D 3 administration could improve the clinical potential of hMPC transplantation currently developed for muscle trauma or myopathies.

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Autologous transplantation of porcine myogenic precursor cells in skeletal muscle

Cited by 34 publications

References 31 publications

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

1,25-Dihydroxyvitamin D₃Increases the Transplantation Success of Human Muscle Precursor Cells in SCID Mice

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Autologous transplantation of porcine myogenic precursor cells in skeletal muscle

Cited by 34 publications

References 31 publications

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Age-related changes in the features of porcine adult stem cells isolated from adipose tissue and skeletal muscle

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

1,25-Dihydroxyvitamin D3Increases the Transplantation Success of Human Muscle Precursor Cells in SCID Mice

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Product

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1,25-Dihydroxyvitamin D₃Increases the Transplantation Success of Human Muscle Precursor Cells in SCID Mice