Autoluminescent Plants

Krichevsky, Alexander; Meyers, Benjamin; Vainstein, Alexander; Maliga, Pál; Citovsky, Vitaly

doi:10.1371/journal.pone.0015461

Cited by 82 publications

(90 citation statements)

References 27 publications

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“…3 The only other light-emitting plant in the literature was reported by Science in 1986, and required 24 h to integrate enough light for imaging. 2 The model in eq 5 suggests that in this work, the I max values are 2 orders of magnitude below the predicted maximum (Figure 4g).…”

Section: * S Supporting Informationmentioning

confidence: 99%

A Nanobionic Light-Emitting Plant

et al. 2017

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The engineering of living plants for visible light emission and sustainable illumination is compelling because plants possess independent energy generation and storage mechanisms and autonomous self-repair. Herein, we demonstrate a plant nanobionic approach that enables exceptional luminosity and lifetime utilizing four chemically interacting nanoparticles, including firefly luciferase conjugated silica (SNP-Luc), D-luciferin releasing poly(lactic-co-glycolic acid) (PLGA-LH 2 ), coenzyme A functionalized chitosan (CS-CoA) and semiconductor nanocrystal phosphors for longer wavelength modulation. An in vitro kinetic model incorporating the release rates of the nanoparticles is developed to maximize the chemiluminescent lifetimes to exceed 21.5 h. In watercress (Nasturtium off icinale) and other species, the nanoparticles circumvent limitations such as luciferin toxicity above 400 μM and colocalization of enzymatic reactions near high adenosine triphosphate (ATP) production. Pressurized bath infusion of nanoparticles (PBIN) is introduced to deliver a mixture of nanoparticles to the entire living plant, well described using a nanofluidic mathematical model. We rationally design nanoparticle size and charge to control localization within distinct tissues compartments with 10 nm nanoparticles localizing within the leaf mesophyll and stomata guard cells, and those larger than 100 nm segregated in the leaf mesophyll. The results are mature watercress plants that emit greater than 1.44 × 10 12 photons/sec or 50% of 1 μW commercial luminescent diodes and modulate "off" and "on" states by chemical addition of dehydroluciferin and coenzyme A, respectively. We show that CdSe nanocrystals can shift the chemiluminescent emission to 760 nm enabling nearinfrared (nIR) signaling. These results advance the viability of nanobionic plants as self-powered photonics, direct and indirect light sources.

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Section: * S Supporting Informationmentioning

confidence: 99%

A Nanobionic Light-Emitting Plant

et al. 2017

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“…The benefits of each idea were experimented recently by insertion of the lux operon with an same expression cassette at both sites and the transcriptionally-active spacer region was found to suggestion a 25-fold higher level of expression [KRICHEVSKY et al, 2010] , and authors ascribed this to higher readthrough transcriptional activity.…”

Section: Cloning Of Rrn23-rrn5 Regionmentioning

confidence: 99%

CLONING THE COTTON rrn23–rrn5 REGION FOR DEVELOPING A UNIVERSAL INTERFAMILY PLASTIDIAL VECTOR

Ghasemi¹,

Kohnehrouz²

2016

bjbabe

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Abstract. Although plastid transformation has attractive advantages in plant biotechnology, it has been highly proficient only in tobacco. The lack of efficient semi lethal selection procedure along with the inefficient recombination through heterologous flanking regions used in transformation vectors prevented this technology to major crops. However, due to the published plastidial genomic sequences and the distinct features of their genomic parts lead the scientists to use of specific and new regions in transformation vectors. In this work, we worked out rrn23-rrn5 fragment as new flanking regions for Malvaceae, Caricaseae, Rutaceae and Solanaceae families by nucleotide homology search. Therefore, the PCR-amplified rrn23-rrn5 region was cloned into the cloning vector using competent cells of E.coli strains DH5α. The sequencing results showed the high homology compared to chloroplast genome of Gossypium sp. with identities of 99 % values.

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“…Replacement of the tobacco rbcL plastid gene (T-rbcL), encoding the Rubisco large subunit, with the sunflower (Helianthus annuus) large subunit (S-rbcL) is shown in Figure 3A (Sharwood et al, 2008). The example shown in Figure 3B is insertion of aadA and six genes (approximately 6.5 kb) of the luciferase (lux) operon in the plastid genome in the trnI-trnA intergenic region (Krichevsky et al, 2010). Thus far, this is the highest number of genes inserted in the ptDNA.…”

Section: The Technology Of Plastid Transformationmentioning

confidence: 99%

“…B, Insertion of the lux operon in the trnI/trnA intergenic region. Note that the lux operon is transcribed from the aadA promoter and the gene cluster has only a single 3# UTR (Krichevsky et al, 2010). available in plastid transformation vectors that also function as E. coli cloning vectors, so that transformation-ready vectors can be obtained in one cloning step.…”

Section: Engineering Of Plastid Transgenes For High-level Protein Expmentioning

confidence: 99%

“…Construction of truly autonomously luminescent plants required the expression of a fully functional bacterial luciferase pathway consisting of six genes (luxCDABEG; Krichevsky et al, 2010). Again, luxC, the first gene of the lux operon, was expressed from the plastid PrrnLrbcL promoter/leader cassette, and the rest of the lux operon genes were expressed from their native translation control sequences.…”

Section: Metabolic Pathway Engineeringmentioning

confidence: 99%

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