Autolytic ultrastructural changes in rat and human hepatocytes

Karadzic, Radovan; Ilić, Goran; Antović, Aleksandra; Banovic, Lidija Kostic

doi:10.4323/rjlm.2010.247

Cited by 11 publications

(8 citation statements)

References 13 publications

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“…It is also easily accessible for pancreatic enzymes and gallbladder fluids, potentially resulting in rapid autolysis [9]. Only a few previous studies of human livers have been carried out evaluating post-mortem histological changes and their potential association with the PMI [10][11][12]. However, these studies have focused on the first few days after death.…”

Section: Introductionmentioning

confidence: 99%

Histological quantification of decomposed human livers: a potential aid for estimation of the post-mortem interval?

et al. 2020

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The objective of this study was to determine if a novel scoring-based model for histological quantification of decomposed human livers could improve the precision of post-mortem interval (PMI) estimation for bodies from an indoor setting. The hepatic decomposition score (HDS) system created consists of five liver scores (HDS markers): cell nuclei and cell structure of hepatocytes, bile ducts, portal triad, and architecture. A total of 236 forensic autopsy cases were divided into a training dataset (n = 158) and a validation dataset (n = 78). All cases were also scored using the total body score (TBS) method. We specified a stochastic relationship between the log-transformed accumulated degree-days (log10ADD) and the taphonomic findings, using a multivariate regression model to compute the likelihood function. Three models were applied, based on (i) five HDS markers, (ii) three partial body scores (head, trunk, limbs), or (iii) a combination of the two. The predicted log10ADD was compared with the true log10ADD for each case. The fitted models performed equally well in the training dataset and the validation dataset. The model comprising both scoring methods had somewhat better precision than either method separately. Our results indicated that the HDS system was statistically robust. Combining the HDS markers with the partial body scores resulted in a better representation of the decomposition process and might improve PMI estimation of decomposed human remains.

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Section: Introductionmentioning

confidence: 99%

Histological quantification of decomposed human livers: a potential aid for estimation of the post-mortem interval?

et al. 2020

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“…Karadzic et al . [ 22 ] observed similar changes in human hepatocytes at 6 h since death which was stored at 18°C.…”

Section: Discussionmentioning

confidence: 73%

Estimation of time since death based on light microscopic, electron microscopic, and electrolyte analysis in the gingival tissue

Muthukrishnan

Narasimhan

Paranthaman

et al. 2018

J Forensic Dent Sci

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Background:Estimation of time since death is an important parameter in forensic science. Although there are various methods available, precise estimation is still to be established.Aim:The present study aimed to evaluate the histological and ultrastructural changes in the gingival tissue along with the changes in electrolyte levels (sodium, potassium, calcium, and magnesium) among the three groups which included normal, 2, and 4 h since death.Materials and Methods:For light microscopic examination and electrolyte analysis, five normal gingival tissue samples were collected from patient following impaction procedure and five gingival tissue samples were obtained from postmortem specimen at 2 and 4 h since death. Each sample was divided into two parts. The first part was fixed in 10% formalin solution for the light microscopic analysis, and microscopic changes were observed between the groups. The second part was snap frozen at −80°C, until measurement of electrolyte using inductively coupled plasma-optical emission spectrometer, and the values were compared among the groups using Kruskal–Wallis test. For electron microscopic examination 2 and 4 h postmortem, gingival tissue samples were collected from the same individual and immediately fixed in 2.5% buffered glutaraldehyde, and the ultrastructural changes were compared with the normal gingival tissue.Results:The light microscopic changes were observed as early as 2 h since death, but there was no significant difference observed between 2 and 4 h postmortem samples whereas ultrastructurally significant difference in morphology was observed between 2 and 4 h postmortem gingival tissue. Our results can confirm histomorphological changes within 2 and 4 h since death.

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“…Before generalising the present findings to other animal species, the size of the animal, type of fur/ feathers and where the testes are situated should be considered, as these may affect autolysis. As an example, autolysis of human liver is slower than autolysis of rat liver at 18°C [ 47 ]. The appearance of autolysis can also change depending on the organ investigated [ 48 ], an effect which should be taken into consideration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Pre-Fixation Delay and Freezing on Mink Testicular Endpoints for Environmental Research

et al. 2015

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There is growing interest in using wild animals to monitor the real-life cocktail effect of environmental chemicals on male reproduction. However, practical difficulties, such as long distances to the laboratory, generally prolong the time between euthanisation and specimen handling. For instance, tissue fixation is often performed on frozen material or on material where deterioration has started, which may affect tissue morphology. This study examined the effect of pre-fixation delay and freezing on mink testicular endpoints in order to determine robust endpoints in suboptimally handled specimens. Sexually mature farmed mink (n=30) selected at culling were divided into six groups and subjected to different time intervals between euthanisation and fixation or freezing: 0 hours (fixed immediately post mortem), 6 hours, 18 hours, 30 hours, 42 hours, or frozen 6 hours post mortem and thawed overnight. Unaffected endpoints when pre-fixation storage was extended to 30 hours included: area and diameter of the seminiferous tubules, length and weight of the testes, and acrosomes marked with Gata-4. Epithelial height, Sertoli cells marked with Gata-4 and cell morphology were affected endpoints after 6 hours of storage. Freezing the tissue prior to fixation severely altered cell morphology and reduced testicular weight, tubular diameter and area. Morphological changes seen after 6 hours included shredded germ cells and excess cytoplasm in seminiferous tubular lumen, chromatin rearrangements and increased germ cell death. Extended delay before fixation and freezing affected many endpoints in the mink testicular tissue. Some of these endpoints may mimic chemically induced effects, which is important to consider when evaluating specimens from wild animals for environmental toxicity.

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Autolytic ultrastructural changes in rat and human hepatocytes

Cited by 11 publications

References 13 publications

Histological quantification of decomposed human livers: a potential aid for estimation of the post-mortem interval?

Histological quantification of decomposed human livers: a potential aid for estimation of the post-mortem interval?

Estimation of time since death based on light microscopic, electron microscopic, and electrolyte analysis in the gingival tissue

Effect of Pre-Fixation Delay and Freezing on Mink Testicular Endpoints for Environmental Research

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