Automated 10-channel capillary chip immunodetector for biological agents detection

Yacoub-George, Erwin; Hell, Waltraud; Meixner, Leonhard; Wenninger, Franz; Bock, Karlheinz; Lindner, Petra; Wolf, Hans; Kloth, Tanja; Feller, Klaus A

doi:10.1016/j.bios.2006.06.003

Cited by 47 publications

(40 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We operate several devices in parallel to provide simultaneous data for a control biofilm channel and for channels with biofilms undergoing different treatments. Streamlined fabrication methods also make this system compatible with integrated sensing techniques, such as electrochemical (Baldrich et al 2011;Ben-Yoav et al 2011), mechanical (Burg et al 2007;Gfeller et al 2005), and optical sensing (Renzi et al 2005;Yacoub-George et al 2007). Such integrated platforms, or "Lab-on-a-Chip" devices, are capable of sample analysis without reliance on external sample preparation or metrology.…”

Section: Introductionmentioning

confidence: 98%

AI-2 analogs and antibiotics: a synergistic approach to reduce bacterial biofilms

Roy

Meyer

Smith

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Quorum sensing (QS), the process of autoinducer-mediated cell-cell signaling among bacteria, facilitates biofilm formation, virulence, and many other multicellular phenotypes. QS inhibitors are being investigated as antimicrobials because of their potential to reduce symptoms of infectious disease while slowing the emergence of resistant strains. Autoinducer-2 (AI-2) analogs have been shown to inhibit genotypic QS responses among many bacteria. We demonstrate for the first time, the ability of C1-alkyl AI-2 analog, isobutyl-DPD, to significantly inhibit the maturation of Escherichia coli biofilms grown in vitro. Using a novel microfluidic device that incorporates dynamic, real-time measurements of biofilm density, we also show that a combinatorial approach wherein isobutyl-DPD ((S)-4,5-dihydroxy-2,3-pentanedione) is used with the antibiotic gentamicin is quite effective in rendering near complete clearance of pre-existing E. coli biofilms. Similarly, another AI-2 analog, phenyl-DPD, also used in combination with near MIC levels of gentamicin, resulted in clearance of preformed Pseudomonas aeruginosa biofilms. Clearance of pre-existing biofilms has remained a significant health care challenge; these results warrant consideration of a new approach based on the combination of "quenching" QS signal transduction processes with traditional antibiotic treatment.

show abstract

Section: Introductionmentioning

confidence: 98%

AI-2 analogs and antibiotics: a synergistic approach to reduce bacterial biofilms

Roy

Meyer

Smith

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Other investigators have reported that the KPL goat anti-E. coli O157:H7 polyclonal antibody is highly specific for E. coli O157:H7. Its cross-reactivity to other bacteria genus such as S. enteritidis 4931, S. typhimirium 13311 (Moore and Griffith 2002;Su and Li 2004;Lee et al 2006), staphylococcal enterotoxin B (Yacoub-George et al 2007), L. monocytogenes (Shriver-Lake et al 2007) and other E. coli stains such as E. coli B, CDC, Crook, O111:B4, O26:B6, W (Yu and Bruno 1996), O55 (Subramanian et al 2006), K-12 (Yu and Bruno 1996;Shriver-Lake et al 2007), HB101, F18 and C25 (Lin et al 2005) are minimal.…”

Section: Specificitymentioning

confidence: 97%

Rapid detection of pathogens using antibody-coated microbeads with bioluminescence in microfluidic chips

Guan

Zhang

et al. 2010

Biomed Microdevices

View full text Add to dashboard Cite

Detection of pathogens was demonstrated in a polydimethylsiloxane (PDMS)/glass microfluidic chip with which microbead-based immunoseparation platform and the bioluminescence technology were integrated. Escherichia coli (E. coli) O157:H7 was used as the model bacteria. The microchamber in microfluidic chip was filled with glass beads coated with antibodies which could capture specific organism, and the capture efficiency of the chip for the bacteria was about 91.75% approximately 95.62%. Then the concentration of bacteria was determined by detecting adenosine triphosphate (ATP) employing bioluminescence reaction of firefly luciferin-lucifera-ATP on chip. The method allowed reliable detection of E. coli O157:H7 concentrations from 3.2 x 10(1) cfu/microL to 3.2 x 10(5) cfu/microL within 20 min. This research demonstrated excellent reproducibility, stability, and specificity, and could accurately detect the pathogenic bacteria in food samples. The microfluidic chip and the equipments used in this method are easy to miniaturize, thus the method has great potential to be developed to a portable device for rapid detection of pathogens.

show abstract

“…Research on implantable nano-enabled biosensors for pathogen and contaminant detection has not yet resulted in applications; recently however, biochips have shown promising progress towards recognition of multiple biological agents [67]. Biosensors and biochips (i.e.…”

Section: Nano-enabled Diagnostics For Real-time Disease Monitoringmentioning

confidence: 99%

An Ethical Argument in Favor of Nano-enabled Diagnostics in Livestock Disease Control

2008

View full text Add to dashboard Cite

show abstract

Automated 10-channel capillary chip immunodetector for biological agents detection

Cited by 47 publications

References 21 publications

AI-2 analogs and antibiotics: a synergistic approach to reduce bacterial biofilms

AI-2 analogs and antibiotics: a synergistic approach to reduce bacterial biofilms

Rapid detection of pathogens using antibody-coated microbeads with bioluminescence in microfluidic chips

An Ethical Argument in Favor of Nano-enabled Diagnostics in Livestock Disease Control

Contact Info

Product

Resources

About