Automated analysis of free and total concentrations of three antiepileptic drugs in plasma with on-line dialysis and high-performance liquid chromatography

Johansen, Karianne; Krogh, Mette; Andresen, Alf T.; Christophersen, Asbjørg S.; Lehne, Gustav; Rasmussen, Knut

doi:10.1016/0378-4347(95)93203-4

Cited by 39 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This drug is mainly bound in blood to the protein human serum albumin (HSA), with approximately 90% of phenytoin being complexed at therapeutic levels in adults [8][9][10][11][12][13][14]. Two clinical situations in which the bound fraction of phenytoin might decrease include infants with jaundice (due to the competition of bilirubin with phenytoin for HSA) and patients that have low HSA levels following trauma or surgery [9].…”

Section: Introductionmentioning

confidence: 99%

“…There have been numerous methods developed for the measurement of phenytoin's free fraction in blood, plasma or serum [6,10,12,[15][16][17][18][19][20][21][22]. Examples include techniques based on equilibrium dialysis, ultrafiltration, and restricted access media (RAM) columns [6,10,14,15,18,19,21].…”

Section: Introductionmentioning

confidence: 99%

“…Examples include techniques based on equilibrium dialysis, ultrafiltration, and restricted access media (RAM) columns [6,10,14,15,18,19,21]. Other techniques such as CE can also be used to examine drug-protein interactions in standard solutions [23][24][25][26], but many of these methods are not applicable for work with the small free fractions of drugs or the complex matrices that are found in clinical samples.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay

2006

View full text Add to dashboard Cite

A chromatographic method was developed for measuring free drug fractions based on the use of an ultrafast immunoextraction/displacement assay (UFIDA) with near-infrared (NIR) fluorescent labels. This approach was evaluated by using it to determine the free fraction of phenytoin in serum or samples containing the binding protein human serum albumin (HSA). Items considered in the design of this method included the dissociation rate of HSA-bound phenytoin, the rate of capture of free phenytoin by immunoextraction microcolumns, the behavior of NIR fluorescent labels in a displacement format, and the overall response and stability of the resulting assay. In the final UFIDA method, the free fraction of phenytoin was extracted in approximately 100 ms by a microcolumn containing a small layer of anti-phenytoin antibodies. This gave a displacement peak for a NIRfluorescent labeled analog of phenytoin that appeared within 2−3 min of sample injection, creating a signal proportional to the amount of free phenytoin in the sample. The UFIDA method provided results within 1−5% of those determined by ultrafiltration for reference samples. The lower limit of detection was 570 pM and the linear range extended up to 10 μM. This approach is not limited to phenytoin but can be adapted for other analytes through the use of appropriate antibodies and labeled analogs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay

2006

View full text Add to dashboard Cite

show abstract

“…It is based on the ASTEDTM system (Automated Sequential Trace Enrichment of Dialysate), connected on-line with an HPLC system. The ASTED system has previously been utilized in several automated methods for analysis of drugs in biological material, in combination with both HPLC and gas chromatography (Agasaster and Rasmussen, 1991a,b;Andresen et al, 1992Andresen et al, , 1993Krogh and Christophersen, 1993;Johansen et al, 1995). Mixtures of chiral compounds present a special problem when dynamic dialysis is used for sample preparation since protein binding, and thus the dialysis rate, is often different for the enantiomers (Williams and Lee, 1985).…”

Section: Levosimendan (R)-( -)-[[4-(1456-tetrahydro-4-methyl-6mentioning

confidence: 99%

Automated Analysis of Levosimendan in Human Plasma by On-Line Dialysis and Liquid Chromatography

Karlsson

Korkolainen

Wikberg

1997

Biomed. Chromatogr.

View full text Add to dashboard Cite

A fully automated method for quantitation of levosimendan, (R)‐(−)‐[[4‐(1,4,5,6‐tetrahydro‐4‐methyl‐6‐oxo‐3‐pyridazinyl)phenyl]hydrazono]propanedinitrile, in human plasma is described. The method involves on‐line dialysis of the samples, trace enrichment of the dialysates, and reversed‐phase high‐performance liquid chromatography with UV detection at 380 nm. An internal standard was used to compensate for variations in the dialysis rate caused by temperature fluctuations. The precision and accuracy of the method were good. The between‐day variation (RSD) was 2.7% at a plasma concentration of 15 ng/mL and 1.7% at 450 ng/mL. The limit of quantitation was 5 ng/mL with an RSD of 4.0%. The completion time of the assay was 19 min. © 1997 by John Wiley & Sons, Ltd.

show abstract

“…The HPLC procedures reported for determining phenobarbital require sample pre-treatments such as protein precipitation (Perry et al, 1992), solvent extraction (Ohmacht et al, 1991;Kuroda et al, 1996), solidphase extraction (Kuono et al, 1993;Moriyama et al, 1994), dialysis (Johansen et al, 1995), or the use of column-switching techniques (Lubda et al, 1996;Kouno et al, 1997). Special chromatographic columns which allow the direct injection of the physiological samples have also been proposed (Perry et al, 1992;Haginaka and Wakai, 1992;Nimura et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Determination of phenobarbital in plasma by micellar liquid chromatography

Martı́n-Biosca

Sagrado

Villanueva-Camañas

et al. 2000

Biomed. Chromatogr.

View full text Add to dashboard Cite

Automated analysis of free and total concentrations of three antiepileptic drugs in plasma with on-line dialysis and high-performance liquid chromatography

Cited by 39 publications

References 21 publications

Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay

Analysis of Free Drug Fractions Using Near-Infrared Fluorescent Labels and an Ultrafast Immunoextraction/Displacement Assay

Automated Analysis of Levosimendan in Human Plasma by On-Line Dialysis and Liquid Chromatography

Determination of phenobarbital in plasma by micellar liquid chromatography

Contact Info

Product

Resources

About