Automated analysis of the pyridinium crosslinks of collagen in tissue and urine using solid-phase extraction and reversed-phase high-performance liquid chromatography

Pratt, David A.; Daniloff, Yuri; Duncan, Alexander; Robins, Simon P.

doi:10.1016/0003-2697(92)90519-d

Cited by 155 publications

(78 citation statements)

References 17 publications

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“…The analysis was performed using highperformance liquid chromatography, as previously described (17). Urinary creatinine levels were measured with a Kone autoanalyzer, using the Jaffe method (18).…”

Section: Methodsmentioning

confidence: 99%

Biomarkers predict radiographic progression in early rheumatoid arthritis and perform well compared with traditional markers

Young-Min¹,

Cawston²,

Marshall³

et al. 2007

Arthritis & Rheumatism

113

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Objective. To evaluate the performance of biochemical and traditional markers in predicting radiographic progression in rheumatoid arthritis (RA).Methods. One hundred thirty-two patients with early RA were treated with nonbiologic therapies for 2 years and studied longitudinally. Genomic DNA was analyzed for presence of the shared epitope. Conclusion. These results indicate that biochemical markers are useful predictors of radiographic progression in RA and that serum MMP-3 levels decrease significantly with MTX therapy. Multivariate models that include MMP-3 and CTX-II perform better than existing traditional markers in predicting radiographic outcome in RA.

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Section: Methodsmentioning

confidence: 99%

Biomarkers predict radiographic progression in early rheumatoid arthritis and perform well compared with traditional markers

Young-Min¹,

Cawston²,

Marshall³

et al. 2007

Arthritis & Rheumatism

113

View full text Add to dashboard Cite

show abstract

“…In brief, portions of pooled urine (500 ml) were first hydrolysed with an equal volume of 12 M HCl at 1071C for 18 hours. The crosslinks from urine hydrolysates were then extracted with cellulose partition chromatography, with the use of an internal standard (acetylated pyridinoline (PYD); MetraBiosystems Ltd, Wheatley, Oxon., UK) (Pratt et al, 1992). The acetylated PYD was used in accordance with the method described by Calabresi et al (1994) and Robins et al (1994).…”

Section: Experimental Techniquesmentioning

confidence: 99%

Effect of dietary supplementation with conjugated linoleic acid on markers of calcium and bone metabolism in healthy adult men

et al. 2005

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Introduction: Conjugated linoleic acid (CLA) has been shown to positively influence calcium and bone metabolism in experimental animals and cells in culture, but there are limited human data available. Objective: To investigate the effect of CLA supplementation on biomarkers of calcium and bone metabolism in healthy adult males. Design: The study consisted of a double-blind, placebo-controlled trial in which 60 healthy adult males (aged 39-64 y) were randomly assigned to receive daily either 3.0 g CLA isomer blend (50:50% cis-9,trans-11:trans-10,cis-12 isomers) or a palm/bean oil blend (placebo) for 8 weeks. Urine and blood samples were collected at weeks 0 and 8 and were analysed for biomarkers of calcium and bone metabolism. Results: Supplementation with CLA or placebo for 8 weeks had no significant effects on markers of bone formation (serum osteocalcin and bone-specific alkaline phosphatase) or bone resorption (serum C-telopeptide-related fraction of type 1 collagen degradation products, urinary N-telopeptide-related fraction of type 1 collagen degradation products, urinary pyridinoline and deoxypyridinoline), or on serum or urinary calcium levels. Baseline levels of these biochemical parameters were similar in both groups of subjects. While the placebo had no effect, CLA supplementation resulted in a three-fold increase (Po0.00001) in cis-9,trans-11 CLA isomer in total plasma lipids.Conclusion: Under the conditions tested in this double-blind, placebo-controlled trial in adult men, a CLA supplement of mixed isomers did not affect markers of calcium or bone metabolism. Further investigation of the effects of CLA on calcium and bone metabolism in other gender-and age-groups is warranted. Sponsorship: Irish Government.

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“…Urinary PYR and DPD were measured by highperformance liquid chromatography after acid hydrolysis using a Gilson ASPEC (automated sample preparation with extraction column; Gilson Inc, Middleton, WI) according to Pratt et al [23]. In our protocol, the intraassay and interassay coefficients of variation were 6.4% and 5.9% for PYR and 6.0% and 6.0% for DPD, respectively.…”

Section: Methodsmentioning

confidence: 99%

Sequential Changes of Bone Metabolism in Normal and Delayed Union of the Spine

Ohishi

Takahashi

Yamanashi

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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Time-dependent changes in bone markers in delayed or nonunion of vertebral fracture were compared with those of normal union. Thirty-three patients with a fresh vertebral fracture were enrolled. Urinary Type I collagen C-terminal telopeptide, pyridinoline, deoxypyridinoline, serum C-terminal telopeptide, and N-midportion of osteocalcin (OC N-mid ) were determined at the time of hospital admission (within 24 hours after the fracture event in all cases) and at 2, 4, 12, 24, and 48 weeks thereafter.

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Automated analysis of the pyridinium crosslinks of collagen in tissue and urine using solid-phase extraction and reversed-phase high-performance liquid chromatography

Cited by 155 publications

References 17 publications

Biomarkers predict radiographic progression in early rheumatoid arthritis and perform well compared with traditional markers

Biomarkers predict radiographic progression in early rheumatoid arthritis and perform well compared with traditional markers

Effect of dietary supplementation with conjugated linoleic acid on markers of calcium and bone metabolism in healthy adult men

Sequential Changes of Bone Metabolism in Normal and Delayed Union of the Spine

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