Automated and Manual Systems for Antimicrobial Susceptibility Testing of Bacteria

Evangelista, Alan T.; Karlowsky, James A.

doi:10.1002/9781119021872.ch22

Cited by 4 publications

(4 citation statements)

References 69 publications

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“…An authenticated modified and verified cascaded series enrollments were dependent for establishing a net framework built-in design [8][9][10][11][12][13][14][15][16][17][18] in which two-hundred and twenty-five pooled samples were collected and verified from sectors of Al-Fudhaliyah, Al-Sadrya and Abu-Ghraib from January to March (2022). An authenticated, modified and verified cascaded series for isolation, identification and confirmation depended on this torment.…”

Section: Special Topics Built-in Designmentioning

confidence: 99%

Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad

Al-Shammary¹,

Mounam²

2023

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Influential, organized groups with natural antimicrobial and anti-biofilm broad-spectrum power exist within the food chain, like a hidden dormant mimic hygienic bio life nanobodies that can terminate multiple opportunistic disease entities owing multi-stress resistant forbidden recalcitrant power, such as Candida albicans. These wonderful dynamic forces created by ALLAH Almighty are the Mycophages or fungi-eating state of fungi foodborne phages, and this project was redirected to be a dare to leap from us towards the future. Multi-stress resistant C. albicans that are resistant to different antifungal agents with their genetic tolerance plasticity to thermal pasteurization decontamination module as well as to ultraviolet irradiation hurdle strategy recovered from raw milk (mastitis), yogurt and soft cheese with versatile phenotypes resident in topic sectors of Abu-Ghraib, Al-Fudhaliyah and Al-Sadrya in Baghdad. From the other side of trueness, we discover an abnormal deviated activity of bacteriophages cocktails that behave with broad-spectrum functions against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Streptococci (VRE) as lytic bactericidal and versus multi stress resistant C. albicans as redirected terminator lytic Mycophages thus objected to be a new nano-built hygienic phenomenon entity (Exodia). Keywords: Exodia, Lytic Mycophages, Multi stress-resistant Candida albicans, dairy chain ecosystems

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Section: Special Topics Built-in Designmentioning

confidence: 99%

Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad

Al-Shammary¹,

Mounam²

2023

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“…Chromogenic substrates are also compatible with broth dilution methods, which are the core of many successful commercial AST platforms, such as the BD Phoenix system (BD; Franklin Lakes, NJ, USA), the MicroScan Walkaway system (Beckman-Coulter; Brea, CA, USA), the VITEK 2 System (BioMérieux), and the Sensititer system, (ThermoFisher Scientific; Waltham, MA, USA). These systems test colony isolate samples suspended in media to a 0.5 McFarland turbidity standard and test against CLSI, EUCAST or FDA standards over a period of 6–24 h through optical measurements of media turbidity and coloration . Reveal from Specific Diagnostics (Mountain View, CA, USA) is a small-molecule sensor array capable of colorimetric detection of volatile organic compounds produced by bacterial metabolic activity.…”

Section: Phenotypic Antimicrobial Susceptibility Testing For Antimicr...mentioning

confidence: 99%

“…These systems test colony isolate samples suspended in media to a 0.5 McFarland turbidity standard and test against CLSI, EUCAST or FDA standards over a period of 6−24 h through optical measurements of media turbidity and coloration. 32 Reveal from Specific Diagnostics (Mountain View, CA, USA) is a small-molecule sensor array capable of colorimetric detection of volatile organic compounds produced by bacterial metabolic activity. The array sheets are designed for use with 96-well broth microdilutions using colony isolates and produce SIR profiles within 5 h.…”

mentioning

confidence: 99%

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

et al. 2021

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The spread of antimicrobial resistance (AMR) is a rapidly growing threat to humankind on both regional and global scales. As countries worldwide prepare to embrace a One Health approach to AMR management, which is one that recognizes the interconnectivity between human, animal, and environmental health, increasing attention is being paid to identifying and monitoring key contributing factors and critical control points. Presently, AMR sensing technologies have significantly progressed phenotypic antimicrobial susceptibility testing (AST) and genotypic antimicrobial resistance gene (ARG) detection in human healthcare. For effective AMR management, an evolution of innovative sensing technologies is needed for tackling the unique challenges of interconnected AMR across various and different health domains. This review comprehensively discusses the modern state-of-play for innovative commercial and emerging AMR sensing technologies, including sequencing, microfluidic, and miniaturized point-of-need platforms. With a unique view toward the future of One Health, we also provide our perspectives and outlook on the constantly changing landscape of AMR sensing technologies beyond the human health domain.

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“…This information is most important for physicians and prevents false negative results with yet unknown resistance genes [ 9 ]. To date, these systems require a preculture (16–24 h) of the patient samples [ 10 ]. In the field of microfluidics, many approaches have recently been taken to improve the accuracy and measurement speed of phenotypic ASTs [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofluidic Immobilization and Growth Detection of Escherichia coli in a Chip for Antibiotic Susceptibility Testing

et al. 2020

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Infections with antimicrobial resistant bacteria are a rising threat for global healthcare as more and more antibiotics lose their effectiveness against bacterial pathogens. To guarantee the long-term effectiveness of broad-spectrum antibiotics, they may only be prescribed when inevitably required. In order to make a reliable assessment of which antibiotics are effective, rapid point-of-care tests are needed. This can be achieved with fast phenotypic microfluidic tests, which can cope with low bacterial concentrations and work label-free. Here, we present a novel optofluidic chip with a cross-flow immobilization principle using a regular array of nanogaps to concentrate bacteria and detect their growth label-free under the influence of antibiotics. The interferometric measuring principle enabled the detection of the growth of Escherichia coli in under 4 h with a sample volume of 187.2 µL and a doubling time of 79 min. In proof-of-concept experiments, we could show that the method can distinguish between bacterial growth and its inhibition by antibiotics. The results indicate that the nanofluidic chip approach provides a very promising concept for future rapid and label-free antimicrobial susceptibility tests.

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Automated and Manual Systems for Antimicrobial Susceptibility Testing of Bacteria

Cited by 4 publications

References 69 publications

Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad

Exodia phenomenon of foodborne Mycophages cocktails against chimeric strains of Candida albicans recovered from dairy chain ecosystems in Baghdad

Progressing Antimicrobial Resistance Sensing Technologies across Human, Animal, and Environmental Health Domains

Nanofluidic Immobilization and Growth Detection of Escherichia coli in a Chip for Antibiotic Susceptibility Testing

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