2023
DOI: 10.21203/rs.3.rs-2479371/v1
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Automated and Rapid Chemiluminescence Immunoassay for Cardiac Troponin I Based on Digital Microfluidics

Abstract: Rapid diagnosis of acute myocardial infarction (AMI) is critical in the clinical setting. An automated and rapid chemiluminescence immunoassay system based on digital microfluidics (DMF) is suggested for cardiac troponin I (cTnI). This method involves a double antibody sandwich structure. The cTnI antigen in the sample to be analyzed was captured by magnetic beads encapsulated with the cTnI antibody, which was subsequently recognized by the biotin-labeled secondary antibody. Horseradish peroxidase (HRP)-labele… Show more

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