Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR

Jiang, Yuan; Ji, Baoqing; Zhu, Changqin; Liu, Liqiang; Peng, Chifang; Jin, Min; Qiao, Ruirui; Jin, Zhengyu; Wang, Libing; Zhu, Shuifang; Xu, Chuanlai

doi:10.1016/j.bios.2009.02.015

Cited by 28 publications

(13 citation statements)

References 30 publications

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“…From Figure 2, we can ensure that the prepared gold nanoparticles are very uniform and the diameter of the gold nanoparticles is about 20 nm. The nanoparticles were then used to label the goatÁ anti-rabbit antibody according to the method reported previously (Chen, Jiang et al, 2009;Ma et al, 2009;Peng et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Different methods based on nanomaterials could improve the sensitivity, speed and quantity of the samples of determination (Chen, Bian, et al, 2009;Chen, Jiang, et al, 2009;Ma et al, 2009;Shim, Chen, Doty, Xu, & Kotov, 2008;Xie, Liu, & Xu, 2009). Herein, we report the research work based on the deposition of silver on the surface of the gold nanoparticles which is conjugated with the antibody.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Cui

Chu

et al. 2010

Food and Agricultural Immunology

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An ultrasensitive determination method was developed to detect the trace medroxyprogesterone acetate (MPA) residues in food. The operation procedure was mostly the same as the traditional enzyme-linked immunosorbent assay method. The gold nanoparticle labelled goat anti-rabbit antibody was used here to take the traditional enzyme labelled antibody. Then the gold labelled antibody was used in the immunoassay as usual. The silver was deposited on the surface of the gold nanoparticles on the second antibody. The quantity of the silver on the surface of the gold nanoparticles was dissolved and analysed by the atomic absorption spectrum. Accordingly, the trace analyte MPA could be determined. The range of the determination was from 0.63 to 39.8 ng/ml and the limit of the detection was 0.25 ng/ml.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Cui

Chu

et al. 2010

Food and Agricultural Immunology

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“…Many biological and medical applications via various MNPs have been reported in the fields of single nucleotide polymorphisms typing and pathogen detection [76,77]. Pathogen diagnostic PCRs, which use NA as amplification template and are limited to use in modern laboratories because of the relatively cumbersome and time-consuming NA extraction from organism samples, have gained considerable attention.…”

Section: High-throughput and Automated Diagnostic Pcr Applicationsmentioning

confidence: 99%

Solid‐phase extraction methods for nucleic acid separation. A review

Yue

et al. 2021

J of Separation Science

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The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.

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“…Multiple poisoning events in poultry and instances of allergic contact dermatitis in farmers induced by olaquindox have been reported in many countries (Song et al 2011). The European Union has banned the use of olaquindox since January 1, 1999 because of health concerns over possible carcinogenic, mutagenic, and photoallergic effects of the drug and its metabolites (W. Chen et al 2009). In 2001, Japan also prohibited use of olaquindox in animal feed.…”

Section: Introductionmentioning

confidence: 99%

“…In 2001, Japan also prohibited use of olaquindox in animal feed. It was banned in 2002, but there are still many cases of illegal use in animal production in China (JECFA 1995(JECFA , 1991EC 1998;W. Chen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Development of a Time-Resolved Fluoroimmunoassay for the Rapid Detection of Methyl-3-Quinoxaline-2-Carboxylic Acid in Porcine Tissues

Wei

Niu

et al. 2014

Analytical Letters

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A time-resolved fluoroimmunoassay for the specific determination of methyl-3-quinoxaline-2-carboxylic acid in animal tissues, a marker residue of olaquindox, was developed. The IC 50 of the assay was found to be 1.46 ± 0.19 ng/mL of methyl-3-quinoxaline-2-carboxylic acid in phosphate-buffered saline samples and the detection limit was 0.16 ± 0.03 ng/mL. For porcine liver and muscle samples spiked with 5, 10, and 15 ng/g, the recovery ranges were 95.7-112.3% and 98.5-116.2% and the coefficients of variation were 9.3-11.5% and 8.9-14.2%, respectively. The time-resolved fluoroimmunoassay results correlated well with high performance liquid chromatography results (correlation coefficients of 0.991 for liver and 0.988 for muscle). This study suggests that this method is simple, fast, and sensitive for the high-throughput determination of methyl-3-quinoxaline-2-carboxylic acid in animal tissues.

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Automated and ultrasensitive detection of methyl-3-quinoxaline-2-carboxylic acid by using gold nanoparticles probes SIA-rt-PCR

Cited by 28 publications

References 30 publications

Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Solid‐phase extraction methods for nucleic acid separation. A review

Development of a Time-Resolved Fluoroimmunoassay for the Rapid Detection of Methyl-3-Quinoxaline-2-Carboxylic Acid in Porcine Tissues

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