1995
DOI: 10.1093/clinchem/41.12.1738
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Automated assay of oxygen radical absorbance capacity with the COBAS FARA II

Abstract: Reactive oxygen species are of interest in biology and medicine because of evidence relating them to aging and disease processes. A relatively simple but sensitive and reliable method for quantifying the oxygen radical absorbance capacity (ORAC) of antioxidants in biological tissues has been automated for use with the COBAS FARA II centrifugal analyzer with a fluorescence-measuring attachment. In this assay, beta-phycoerythrin (beta-PE) is used as an indicator protein, 2,2'-azobis(2-amidinopropane) dihydrochlo… Show more

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Cited by 354 publications
(186 citation statements)
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“…Several recent studies have been devoted to the antioxidant capacity in the lipid compartment of plasma (Re et al, 1999;Mayer et al, 2001;Prior et al, 2003) allowing to describe an assay that measures antioxidant capacity in both the hydrophilic and the lipophilic compartments of plasma (Aldini et al, 2001). An important advantage of this assay is that it requires a much lower dilution of plasma (5-10) compared to FRAP (100) (Benzie & Strain, 1996), ORAC (150) (Cao et al, 1995), TRAP (250) (Cao & Prior, 1999) and TAS (120) (Child et al, 1999). Further studies are needed to assess the efficiency of this new assay to explore the postexercise oxidative stress.…”
Section: Discussionmentioning
confidence: 99%
“…Several recent studies have been devoted to the antioxidant capacity in the lipid compartment of plasma (Re et al, 1999;Mayer et al, 2001;Prior et al, 2003) allowing to describe an assay that measures antioxidant capacity in both the hydrophilic and the lipophilic compartments of plasma (Aldini et al, 2001). An important advantage of this assay is that it requires a much lower dilution of plasma (5-10) compared to FRAP (100) (Benzie & Strain, 1996), ORAC (150) (Cao et al, 1995), TRAP (250) (Cao & Prior, 1999) and TAS (120) (Child et al, 1999). Further studies are needed to assess the efficiency of this new assay to explore the postexercise oxidative stress.…”
Section: Discussionmentioning
confidence: 99%
“…A wide range of methods are currently used to assess antioxidant capacity of fruits and vegetables (Halliwell et al, 1995). The most popular being the ORAC (oxygen radical absorbance capacity) method of Cao et al (1995) and Wang et al (1996) which measures total antioxidant capacity in biological systems. This sensitive assay measures the protection provided by antioxidants against either hydroxyl or peroxyl radicals.…”
Section: Antioxidant Evaluation Methodsmentioning
confidence: 99%
“…The presence of different anti-oxidant components in plant tissues especially fruits and vegetables make it relatively difficult to measure each antioxidant component separately. Therefore several methods (Al-Saikhon et al, 1995;Cao et al, 1995; Data expressed as mean ± s.e.m. of three samples analysed separately.…”
Section: Anti-oxidant Activitymentioning
confidence: 99%