Automated determination of drugs in serum by liquid chromatography with column-switching. I. Separation of antiepileptic drugs and metabolites.

Matsumoto, Kazuaki; Kikuchi, Hiroe; Kano, S; Iri, Hisami; Takahashi, Hiyori; Umino, Masuo

doi:10.1093/clinchem/34.1.141

Cited by 22 publications

(1 citation statement)

References 8 publications

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“…Besides RIA, GC, and HPLC methods [24][25][26][27], MEKC has been used to separate and quantify CBZ in serum . The MEKC methods exhibit the advantage of higher selectivity in comparison with other techniques, such as RIA, which overestimates the values of CBZ (giving the sum of CBZ and CBZ-EP).…”

Section: Carbamazepinementioning

confidence: 99%

Analysis of antiepileptic drugs in biological fluids by means of electrokinetic chromatography

Pucci

Raggi

2005

Electrophoresis

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An overview of the electrokinetic chromatographic methods for the analysis of antiepileptic drug levels in biological samples is presented. In particular, micellar electrokinetic capillary chromatography is a very suitable method for the determination of these drugs, because it allows a rapid, selective, and accurate analysis. In addition to the electrokinetic chromatographic studies on the determination of antiepileptic drugs, some information regarding sample pretreatment will also be reported: this is a critical step when the analysis of biological fluids is concerned. The electrokinetic chromatographic methods for the determination of recent antiepileptic drugs (e.g., lamotrigine, levetiracetam) and classical anticonvulsants (e.g., carbamazepine, phenytoin, ethosuximide, valproic acid) will be discussed in depth, and their pharmacological profiles will be briefly described as well.

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Section: Carbamazepinementioning

confidence: 99%

Analysis of antiepileptic drugs in biological fluids by means of electrokinetic chromatography

Pucci

Raggi

2005

Electrophoresis

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Simultaneous determination of mycophenolic acid and valproic acid based on derivatization by high‐performance liquid chromatography with fluorescence detection

Zhong¹,

Jiao²,

Yu³

2005

Biomedical Chromatography

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A reliable and validated reversed-phase high-performance liquid chromatography (HPLC) method using fluorescence detection is reported for the simultaneous quantitation of mycophenolic acid (MPA) and valproic acid (VPA) in human plasma. The method is based on the pre-column derivatization of valproic acid with 4-bromomethyl-6, 7-dimethoxycoumarin (BrMMC) and online solvatochromism of MPA by pH adjustment. The linear calibration range was 0.50-30 microg/mL for MPA and 5.00-150 microg/mL for VPA. The relative standard deviations of the method of intra- and inter-day analyses (n = 6) were below 6.5 and 6.7% for MPA, and 5.8 and 6.3% for VPA, respectively. Dichloromethane was used for the simultaneous extraction of MPA and VPA from acidified plasma. This reliable method can be applied in the analysis of MPA and VPA in human plasma using only a small volume (100 microL).

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Detection of anticonvulsants and their metabolites in urine within a “general unknown” analysis procedure using computerized gas chromatography-mass spectrometry

Maurer

1990

Arch Toxicol

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Detection of the anticonvulsants carbamazepine, clonazepam, diazepam, ethosuximide, mephenytoin, mesuximide, methylphenobarbital, phenobarbital, phenytoin, primidone, propylhexedrine, sultiame, trimethadion and their metabolites in urine is described. The method presented is integrated in a general screening procedure (general unknown analysis) for several groups of drugs, detecting several hundred drugs and over 1000 metabolites. It includes cleavage of conjugates by acid hydrolysis, isolation by liquid-liquid extraction, derivatization by acetylation, separation by capillary gas chromatography and identification by computerized mass spectrometry. Using mass chromatography with the selective ions m/z 58, 104, 113, 117, 165, 193, 204 and 246, the possible presence of anti-convulsants and/or their metabolites was indicated. The identity of positive signals in the reconstructed mass chromatograms was confirmed by a visual or computerized comparison of the stored full mass spectra with the reference spectra. The sample preparation, mass chromatograms, reference mass spectra and gas chromatographic retention indices are documented.

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Automated determination of drugs in serum by liquid chromatography with column-switching. I. Separation of antiepileptic drugs and metabolites.

Cited by 22 publications

References 8 publications

Analysis of antiepileptic drugs in biological fluids by means of electrokinetic chromatography

Analysis of antiepileptic drugs in biological fluids by means of electrokinetic chromatography

Simultaneous determination of mycophenolic acid and valproic acid based on derivatization by high‐performance liquid chromatography with fluorescence detection

Detection of anticonvulsants and their metabolites in urine within a “general unknown” analysis procedure using computerized gas chromatography-mass spectrometry

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