Automated extraction protocol for quantification of SARS-Coronavirus RNA in serum: an evaluation study

Chiu, Rossa W.K.; Jin, Yuanliang; Chung, Grace Tin‐Yun; Lui, Wing Bong; Chan, Anthony T.C.; Lim, Wilina; Lo, Y.M. Dennis

doi:10.1186/1471-2334-6-20

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Earlier reports found that magnetic particle-based automated systems could be inferior [10] , close [11] , [12] or superior [4] to manual column methods for recovering viral RNA from various sample types, moreover, the automated systems generally brought about high precision and hands-on time reduction. Our data demonstrated that Maxwell 16-S RNA extraction had good linearity and precision over a wide concentration range and higher sensitivity in detection of stock flu-v in VTM than the QIAamp method.…”

Section: Discussionmentioning

confidence: 99%

Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples

et al. 2012

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This study evaluated the performance of the Maxwell 16 System (Promega) for extraction of influenza virus (flu-v) RNA from diverse samples compared to a classical manual method (QIAamp Kit, QIAGEN). Following extraction by the two methods, all samples were analyzed by Real-time RT-PCR. Results revealed that the use of the standard Maxwell 16 protocol (Maxwell 16-S) resulted in good linearity and precision across a wide concentration range and higher sensitivity of detection from flu-v stock suspensions than the manual method. Compared with the latter method, Maxwell 16-S extracted RNA more efficiently (higher RNA yield and/or fewer PCR inhibitors) from throat swabs and bronchoalveolar lavage fluids, while both methods performed comparably on fecal samples from human and poultry in terms of overall threshold cycle values and detection rates although the Maxwell 16-S co-purified more inhibitors from fecal samples. The capacity of this system to remove inhibitors from fecal matrix was improved by using a modified Maxwell 16 protocol with a reduced sample input, which eliminated all false-negatives produced by the Maxwell 16-S. These findings suggest that the Maxwell 16 System is suitable for RNA extraction from multiple-source samples for diagnosis of influenza and viral load determination and that a proper reduction in starting sample volume may improve the detection of flu-v from complex matrices such as feces. Additionally, this system allows flexible sample throughput and labor-saving sample processing with little or no risk of cross-contamination.

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Section: Discussionmentioning

confidence: 99%

Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples

et al. 2012

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“…Initially, SARS corona (SARS-CoV) appears as a Flu-like illness that advances to respiratory failure, pneumonia and, in certain instances, death [36] , [37] , [38] , [39] , [40] . SARS-related mortality rate is relatively higher than influenza or other common infections of the respiratory tract [40] , [41] , [42] , [43] , [44] .…”

Section: Sars-covmentioning

confidence: 99%

SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge

2021

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Highlights The highly pathogenic MERS-CoV, SARS-CoV and SARS-CoV-2 cause acute illness and are often fatal. MERS-CoV, SARS-CoV and SARS-CoV-2 tend to infect the respiratory tract and can cause acute respiratory syndrome. SARS-related mortality rate is relatively higher than influenza. CT scans and RT-PCR are the most promising diagnostic tools for COVID-19 diagnosis.

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“…There are many benefits to this approach, including speed, accuracy, and a reduction in the exposure of laboratory personnel to the virus. On the market, there are many RNA extraction apparatuses that could be implemented in SARS-CoV-2 diagnostics, with some examples such as the QIAcube Connect (Qiagen, Hilden, Germany), EZ1 Advanced XL (Qiagen) [ 30 ], NucliSENS easyMAG (bioMerieux, Marcy-l’Étoile, France) [ 10 ], EMAG (bioMerieux) [ 31 ], and also those adapted to higher biosafety levels in instruments such as the MagNA Pure LC (Roche Diagnostics, Basel, Switzerland) [ 32 ].…”

Section: Molecular Biology Techniques Targeting Sars-cov-2 Nucleic Acidsmentioning

confidence: 99%

Role of Laboratory Medicine in SARS-CoV-2 Diagnostics. Lessons Learned from a Pandemic

et al. 2021

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Since the 2019 novel coronavirus outbreak began in Wuhan, China, diagnostic methods in the field of molecular biology have been developing faster than ever under the vigilant eye of world’s research community. Unfortunately, the medical community was not prepared for testing such large volumes or ranges of biological materials, whether blood samples for antibody immunological testing, or salivary/swab samples for real-time PCR. For this reason, many medical diagnostic laboratories have made the switch to working in the field of molecular biology, and research undertaken to speed up the flow of samples through laboratory. The aim of this narrative review is to evaluate the current literature on laboratory techniques for the diagnosis of SARS-CoV-2 infection available on pubmed.gov, Google Scholar, and according to the writers’ knowledge and experience of the laboratory medicine. It assesses the available information in the field of molecular biology by comparing real-time PCR, LAMP technique, RNA sequencing, and immunological diagnostics, and examines the newest techniques along with their limitations for use in SARS-CoV-2 diagnostics.

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Automated extraction protocol for quantification of SARS-Coronavirus RNA in serum: an evaluation study

Cited by 6 publications

References 8 publications

Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples

Performance Evaluation of the Maxwell 16 System for Extraction of Influenza Virus RNA from Diverse Samples

SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge

Role of Laboratory Medicine in SARS-CoV-2 Diagnostics. Lessons Learned from a Pandemic

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