Automated flow-injection immunosensor based on current pulse capacitive measurements

Erlandsson, Dag; Teeparuksapun, Kosin; Mattìasson, Bo; Hedström, Martin

doi:10.1016/j.snb.2013.08.076

Cited by 35 publications

(35 citation statements)

References 25 publications

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“…This system is based on current pulse capacitive measurements, and was described by Erlandsson et al (2014). The feed buffer used was a 10 mM KH 2 PO 4 /K 2 HPO 4 buffer at pH ¼6.32.…”

Section: Automated Flow Injection Systemmentioning

confidence: 99%

“…We have in this work developed an affinity sensor based on capacitive transduction, able to perform as the sensing device (detector) in such a unit. Molecularly imprinted polymers (MIPs) were used as recognition elements, where binding of the analyte to the sensor surface caused a capacitance change, which was further assessed by a constant current pulse to the sensor transducer, as described by Erlandsson et al (2014).…”

Section: Introductionmentioning

confidence: 99%

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Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

Lenain

Saeger

Mattìasson

et al. 2015

Biosensors and Bioelectronics

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“…This system is based on current pulse capacitive measurements, and was described by Erlandsson et al (2014). The feed buffer used was a 10 mM KH 2 PO 4 /K 2 HPO 4 buffer at pH ¼6.32.…”

Section: Automated Flow Injection Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

Lenain

Saeger

Mattìasson

et al. 2015

Biosensors and Bioelectronics

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“…The present work describes a sequential micro flow-ELISA based on amperometric detection using a fully automated and computer-controlled system named VersAFlo. This sequential injection analysis (SIA) system was developed in-house and its operations are described fully by Kumar et al [ 26 ] and its system integration by Erlandsson et al [ 29 ]. The amperometric VersAFlo-ELISA system offers a significantly reduced assay time, real-time information related to the assay as well as high reusability of the immunocolumn.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Flow-ELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System

Lebogang

Jantra

Hedström

et al. 2017

Sensors

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An amperometric immunoanalysis system based on monoclonal antibodies immobilized on Sepharose beads and packed into a micro-immunocolumn was developed for the quantification of microcystin-LR. Microcystin-LR (MCLR) was used as a reference microcystin variant. Inside the immunocolumn, free microcystins and microcystin-horseradish peroxidase (tracer) were sequentially captured by the immobilized antibodies, and the detection was performed electrochemically using Super AquaBlue ELISA substrate 2,2′-azinobis(3-ethylbenzothiazoline-sulfonic acid) (ABTS). The ABTS●+ generated by enzymatic oxidation of ABTS was electrochemically determined at a carbon working electrode by applying a reduction potential set at 0.4 V versus Ag/AgCl reference electrode. The peak current intensity was inversely proportional to the amount of analyte bound to the immunocolumn. The amperometric flow-ELISA system, which was automatically controlled through the CapSenzeTM (Lund, Sweden) computer software, enabled determination of MCLR as low as 0.01 µg/L. The assay time was very short (20 min for one assay cycle). In addition, the electrochemical signals were not significantly affected by possible interferences which could be present in the real samples. Along with the simplicity of automation, this makes the developed method a promising tool for use in water quality assessment.

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“…Contrary to natural antibodies and aptamers (Sellergren 2001;Vlatakis et al 1993), MIPs, which have no 88 biological origin, are robust and characterized by a high mechanical and thermal stability and show an 89 excellent chemical resistance in a broad pH range (Svenson and Nicholls 2001 Capacitive based label-free sensors are one of the unique platforms that do not require complex sample preparation, it provides a stable signal allowing constant read-out and process monitoring (Erlandsson et al, 2014). A typical capacitive sensor sensitively generates the signal upon binding of the target analyte due to changes in electrical double layer composition on the interface between buffer and electrode surface.…”

mentioning

confidence: 99%

“…Electrochemical detection of N-FA was implemented using an automated flow injection system, developed by CapSenze Biosystems AB (Lund, Sweden) and was based on processing the capacitance changes. This approach is based on current pulse capacitive measurements, and was described for the first time by Erlandson et al(Erlandsson et al 2014). Ten mM KH2PO4/K2HPO4 buffer was implemented as a running buffer.…”

mentioning

confidence: 99%

Capacitive sensing of N-formylamphetamine based on immobilized molecular imprinted polymers

Graniczkowska

Pütz

Hauser

et al. 2017

Biosensors and Bioelectronics

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A highly sensitive, capacitive biosensor was developed to monitor trace amounts of an amphetamine precursor in aqueous samples. The sensing element is a gold electrode with molecular imprinted polymers (MIPs) immobilized on its surface. A continuous-flow system with timed injections was used to simulate flowing waterways, such as sewers, springs, rivers, etc., ensuring wide applicability of the developed product. MIPs, implemented as a recognition element due to their stability under harsh environmental conditions, were synthesized using thermo- and UV-initiated polymerization techniques. The obtained particles were compared against commercially available MIPs according to specificity and selectivity metrics; commercial MIPs were characterized by quite broad cross-reactivity to other structurally related amphetamine-type stimulants. After the best batch of MIPs was chosen, different strategies for immobilizing them on the gold electrode's surface were evaluated, and their stability was also verified. The complete, developed system was validated through analysis of spiked samples. The limit of detection (LOD) for N-formyl amphetamine was determined to be 10μM in this capacitive biosensor system. The obtained results indicate future possible applications of this MIPs-based capacitive biosensor for environmental and forensic analysis. To the best of our knowledge there are no existing MIPs-based sensors toward amphetamine-type stimulants (ATS).

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Automated flow-injection immunosensor based on current pulse capacitive measurements

Cited by 35 publications

References 25 publications

Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

Electrochemical Flow-ELISA for Rapid and Sensitive Determination of Microcystin-LR Using Automated Sequential Injection System

Capacitive sensing of N-formylamphetamine based on immobilized molecular imprinted polymers

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