Automated Forensic DNA Purification Optimized for FTA Card Punches and Identifiler STR-based PCR Analysis

Tack, L C; Thomas, Michelle; Reich, Karl

doi:10.1016/j.cll.2006.12.009

Cited by 29 publications

(7 citation statements)

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“…In addition, there is a large array of analytes that can be measured from dried blood spot biospecimens for biochemical markers, mutations, or polymorphisms (9, 10). The extraction of DNA from blood spot cards can be automated (11).…”

Section: Resultsmentioning

confidence: 99%

Blood Collection, Shipment, Processing, and Storage

Vaught

2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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As part of the AACR Annual Meeting Methods Workshop ''Sample Collection, Processing, and Storage for Large-Scale Studies: Biorepositories to Support Cancer Research,'' blood specimen collection, processing, storage, and dissemination issues were discussed. Whole blood and blood fractions comprise a major portion of biospecimen collections for population-based studies. Although procedures for collecting, processing, storing, and shipping blood components are generally standardized and well documented, several important factors need to be considered before a new study involving blood collection is initiated. Blood is collected from study participants for a variety of purposes, including as a source of DNA, and for a variety of laboratory analyses that may be done on whole blood or blood fractions, including serum, plasma, and lymphocytes. Blood or blood fractions may be shipped to laboratories and biorepositories either at ambient temperature, cooled or frozen, depending on the intended analyses. Blood processing may include fractionation, cryopreservation to preserve the viability of lymphocytes, or purification of nucleic acids. The proper storage conditions depend on a variety of factors, including the intended analyses and whether the specimens will be used within a short period or need to be stored for longer periods. As for all laboratory and biorepository procedures, blood collection, shipment, processing, and storage should be conducted under a strict quality assurance program, including standard operating procedures and regular quality control reviews.

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Section: Resultsmentioning

confidence: 99%

Blood Collection, Shipment, Processing, and Storage

Vaught

2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…In 2006 Perrier mentioned -when referring to the Phuket identifications -the effectiveness of the following methods: 73% of identification by odontology, 24% by dactyloscopy and 3% by DNA analysis (Perrier et al, 2006). This shows the need to first focus on simple, inexpensive and quickly efficient methods, without diminishing of course the value of fingerprints and the excellence of the positive DNA identifications (Andersen Torpet, 2005;Biesecker et al, 2005;Brenner, 2006;Deng et al, 2005;Hinchliffe, 2007;Leclair et al, 2004;Leclair et al, 2007;Salo et al, 2007;Schuller-Gotzburg & Suchanek, 2007;Tack et al, 2007;Tsokos et al, 2006). Sribanditmongkol et al reported similar conclusions 6 .…”

Section: Discussion -Guidelinesmentioning

confidence: 99%

“…The predominant role of forensic odontology was also underlined by Kirsch et al (2007). This highlights the need to first focus on simple, inexpensive and time-efficient methods, of course without minimizing the value of fingerprints and the excellence of DNA which are decisive in the identification process (Andersen Torpet, 2005;Biesecker et al, 2005;Brenner, 2006;Deng et al, 2005;Hinchliffe, 2007;Leclair et al, 2004;Leclair et al, 2007;Salo et al, 2007;Schuller-Gotzburg & Suchanek, 2007;Tack et al, 2007;Tsokos et al, 2006). In December 2008, The Thai Tsunami Victim Identification and Repatriation Center mentioned that 388 bodies remain unidentified from a total of 3,696 examined bodies on the Wat Yan Yao Site 1a (Beauthier et al, 2009) 7 .…”

Section: Discussion -Guidelinesmentioning

confidence: 99%

Autopsy and Identification Techniques

Beauthier¹,

Lefèvre²,

Valck³

2011

The Tsunami Threat - Research and Technology

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“…All tests were done in local CDC laboratory using uniform reagents and equipments corrected with standard sample from National CDC lab. A drop of intravenous blood for each patient from the laboratory examination blood sampling was taken and saved in Whatman FTA card [37] for future genetic analysis and DNA banking.…”

Section: Methodsmentioning

confidence: 99%

Design of the Anti-tuberculosis Drugs induced Adverse Reactions in China National Tuberculosis Prevention and Control Scheme Study (ADACS)

Xia

Hu²,

Liu

et al. 2010

BMC Public Health

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BackgroundMore than 1 million tuberculosis (TB) patients are receiving the standard anti-TB treatment provided by China National Tuberculosis Prevention and Control Scheme (CNTS) in China every year. Adverse reactions (ADRs) induced by anti-TB drugs could both do harm to patients and lead to anti-TB treatment failure. The ADACS aimed to explore ADRs' incidences, prognoses, economical and public health impacts for TB patients and TB control, and build a DNA bank of TB patients.Methods/DesignMultiple study designs were adopted. Firstly, a prospective cohort with 4488 sputum smears positive pulmonary tuberculosis patients was established. Patients were followed up for 6-9 months in 52 counties of four regions. Those suspected ADRs should be checked and confirmed by Chinese State Food and Drug Administration (SFDA). Secondly, if the suspected ADR was anti-TB drug induced liver injury (ATLI), a nested case-control study would be performed which comprised choosing a matched control and doing a plus questionnaire inquiry. Thirdly, health economical data of ADRs would be collected to analyze financial burdens brought by ADRs and cost-effectiveness of ADRs' treatments. Fourthly, a drop of intravenous blood for each patient was taken and saved in FTA card for DNA banking and genotyping. Finally, the demographic, clinical, environmental, administrative and genetic data would be merged for the comprehensive analysis.DiscussionADACS will give an overview of anti-TB drugs induced ADRs' incidences, risk factors, treatments, prognoses, and clinical, economical and public health impacts for TB patients applying CNTS regimen in China, and provide suggestions for individualized health care and TB control policy.

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Automated Forensic DNA Purification Optimized for FTA Card Punches and Identifiler STR-based PCR Analysis

Cited by 29 publications

References 1 publication

Blood Collection, Shipment, Processing, and Storage

Blood Collection, Shipment, Processing, and Storage

Autopsy and Identification Techniques

Design of the Anti-tuberculosis Drugs induced Adverse Reactions in China National Tuberculosis Prevention and Control Scheme Study (ADACS)

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