2016
DOI: 10.1177/2211068216632349
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Automated Gravimetric Calibration to Optimize the Accuracy and Precision of TECAN Freedom EVO Liquid Handler

Abstract: High-throughput screening technologies are increasingly integrated into the formulation development process of biopharmaceuticals. The performance of liquid handling systems is dependent on the ability to deliver accurate and precise volumes of specific reagents to ensure process quality. We have developed an automated gravimetric calibration procedure to adjust the accuracy and evaluate the precision of the TECAN Freedom EVO liquid handling system. Volumes from 3 to 900 µL using calibrated syringes and fixed … Show more

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Cited by 22 publications
(13 citation statements)
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“…Anticipating that there would be a need to vary the pipetting air-gap in the future to accomodate different operating volumes, we wished to develop a procedure that would enable quick and reliable determination of calibration parameters for the pipette tips. While automated gravimetric methods have been explored in 12 the past for calibrating liquid handling pipettes, these would require the presence of a specialized, on-deck high-accuracy balance with minimal air-flow to prevent evaporation [34], which may not be available on most liquid handling decks. We expected that the volume estimates calculated using the photometric standard could be used calibrate the pipettes.…”
Section: Automated Photometric Calibration Of Liquid Handling Pipettesmentioning
confidence: 99%
“…Anticipating that there would be a need to vary the pipetting air-gap in the future to accomodate different operating volumes, we wished to develop a procedure that would enable quick and reliable determination of calibration parameters for the pipette tips. While automated gravimetric methods have been explored in 12 the past for calibrating liquid handling pipettes, these would require the presence of a specialized, on-deck high-accuracy balance with minimal air-flow to prevent evaporation [34], which may not be available on most liquid handling decks. We expected that the volume estimates calculated using the photometric standard could be used calibrate the pipettes.…”
Section: Automated Photometric Calibration Of Liquid Handling Pipettesmentioning
confidence: 99%
“…A more sensitive substrate also requires better control on volume of reagents for lower variation. The spiral on the chip also acts as a fixed volume zone for the assay, whereas in a 96 well plate, dispense of particular liquid is dependent on the external system and can easily vary between 1% and 5% for low volume dispense [31][32][33] (1-20 ll) which can give rise to variation in the final assay results.…”
Section: B Spiked Gfap Test With Human Serummentioning
confidence: 99%
“…In general, the confirmation of dispensed volumes in automated liquid handlers is carried out using water or model fluids (with dyes) using gravimetric, fluorometric, or photometric approaches for quantitation. 15 The performance of liquid handlers can differ between liquids with different physical properties, such as surface tension, density, and viscosity. Calibration parameters optimized for water do not necessarily reflect the reality of pipetting different biological fluids.…”
Section: Introductionmentioning
confidence: 99%