Automated high-throughput neurophenotyping of zebrafish social behavior

Green, Jeremy; Collins, Christopher; Kyzar, Evan J.; Pham, Mimi; Roth, Andrew; Gaikwad, Siddharth; Cachat, Jonathan; Stewart, Adam; Landsman, Samuel; Grieco, Fabrizio; Tegelenbosch, Ruud A.J.; Noldus, L.P.J.J.; Kalueff, Allan V.

doi:10.1016/j.jneumeth.2012.07.017

Cited by 159 publications

(104 citation statements)

References 34 publications

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“…An alternative explanation, albeit based on indirect evidence and qualitative observation of zebrafish behavior, may involve "energizing" effects of mescaline (Kalueff et al, 2011−2013, own unpublished observation), which can contribute to higher probability of individual fish running into each other when assessed together in a relatively small and narrow SBT tank. Analyses of the effect of MDMA on shoaling behavior 126 reveal a marked reduction of shoal cohesion, resembling the behavioral effects previously reported for LSD. 66 While the latter effect strikingly differs from well-known pro-social "empathogenic" action of MDMA in humans, 127−129 it resembles the lack of consistent effects of MDMA on social behavior in rodents, 130−135 raising the possibility that the empathogenic action of MDMA may represent a speciesspecific human phenotype, which is difficult to mimic in animal models.…”

Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishsupporting

confidence: 79%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

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Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishsupporting

confidence: 79%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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“…The goal of the two recordings was to study the effects of habituation and/or diminishing effects of the drugs (the half-life of MK-801 in rats is about 2 hr 28 ). We analyzed (1) average social distances (also called inter-individual distances 11,29 ) as measure for social cohesion, (2) vertical distribution over ten depth levels, (3) horizontal distribution over four radial zones (see Figure 24A, inset for locations of zones) and over quadrants (see Figure 24B, inset for locations of quadrants), and (4) the three components (x,y,z) of travel distance. The data were averaged per shoal.…”

Section: Representative Resultsmentioning

confidence: 99%

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Maaswinkel

Zhu

Weng

2013

JoVE

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Like many aquatic animals, zebrafish (Danio rerio) moves in a 3D space. It is thus preferable to use a 3D recording system to study its behavior. The presented automatic video tracking system accomplishes this by using a mirror system and a calibration procedure that corrects for the considerable error introduced by the transition of light from water to air. With this system it is possible to record both single and groups of adult zebrafish. Before use, the system has to be calibrated. The system consists of three modules: Recording, Path Reconstruction, and Data Processing. The step-by-step protocols for calibration and using the three modules are presented. Depending on the experimental setup, the system can be used for testing neophobia, white aversion, social cohesion, motor impairments, novel object exploration etc. It is especially promising as a first-step tool to study the effects of drugs or mutations on basic behavioral patterns. The system provides information about vertical and horizontal distribution of the zebrafish, about the xyz-components of kinematic parameters (such as locomotion, velocity, acceleration, and turning angle) and it provides the data necessary to calculate parameters for social cohesions when testing shoals.

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“…Advances in technology that are being used in other areas of behavioural research offer opportunities for improving experimental designs to allow them to more closely resemble wild conditions. For example, breeding populations of many species could be more accurately recorded by using technology (Sumner et al 2007)/software (Green et al 2012) capable of tracking the movement and association patterns of multiple animals independently over an extended period, allowing the inclusion of several focal and stimulus animals in each trial.…”

Section: A Lack Of Non-laboratory Study Systemsmentioning

confidence: 99%