Automated Instrumentation and the Laboratory Diagnosis of Bleeding and Thrombotic Disorders

Functional Determination of Antithrombin III in Clinical Samples and Therapeutic Concentrates

“…However, immunologic assays are useful in determining variant forms of AT III for more in-depth studies. 10,31 When the functional AT III levels of various commercial AT III concentrates were analyzed, sig nificant differences were found between the as signed potency and the observed levels of AT III levels in these concentrates. Since different com mercial preparations are standardized by in-house methods that may differ, these variations may primarily be due to methodology.…”

From Ink Pens to Computers: A Personal Look Back at Landmark Changes during 5 Decades as a Clinical Laboratory Scientist in U.S. Hemostasis Laboratories

“…Semi-automated analyzers may have been something as simple as the BBL Fibrometer 10 or more “complex” instruments such as the MLA Electra or Coag-A-Mate instrument series. 11 These manual or semi-automated systems required some technical interaction, usually in the form of pipetting patient samples into testing cuvettes, although the fibrometer method required manually pipetting of reagents as well as samples and when using the pipet attached to the device to dispense reagent the timer was automatically initiated. All results for fibrometer testing required manual recording.…”

Automation in Coagulation Testing Clinical Applications

“…18 Automatable immunologic methods have like wise undergone rapid development and many have recently become available for clinical trials. Those of particular interest are the radioimmunoassays, 34 laser nephelometric methods, 34 immunoturbidimetric, 23 and fluoroimmunoassays 34 recently developed. These assays for the antigens of antithrombin III, plasminogen, Cl-INH, Factor VIII RAg, Factor VIII C, Factor IX, and Protein C are of particular interest and are being rapidly developed.…”

“…Functional and immunochemical assays are essential and have been constantly updated for most of these proteins and proteases. 1,7,19,23,34 Synthetic substrate methods that are automatable are of great interest, particularly for Factor VIII, Factor IX, and the vitamin K dependent factors (anticoagulant control). A suitable Factor VIII method has been recently developed.…”