Automated Lab-on-a-Chip Analysis of DNA Fragments

Ausserer, Walter A.; Bousse, Luc; Gallagher, Steven J.; Kennedy, Colin B.; Phan, Huan L.

doi:10.1016/s1535-5535-04-00139-x

Cited by 4 publications

(7 citation statements)

References 6 publications

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“…The latter was required for electrokinetic delivery of samples from the vials into the chip channels. This mode of sample delivery is advantageous in that no extra driving devices were required apart from those normally used for chip-based CE separation, comparing favorably with the system described by Ausserer et al, 24 where a vacuum source was required for sample aspiration. Automated sample presentation system.…”

Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 92%

“…Later, Ausserer et al adopted a similar approach for continuous sample introduction on a chip-based CE system for DNA separation, using vacuum to introduce samples into the sampling channel. 24 In addition to the large dimensional difference of the sampling channel and sample loading/separation channels, a viscous medium was filled into the separation channel that further increased the impedance against pressure-induced flows. Lin et al employed a similar chip design to couple CE microchips with flowthrough analyzers.…”

Section: Introductionmentioning

confidence: 99%

“…The 4-terminal high-voltage power supply used for CE separation was used for sample introduction without additional driving device for sample introduction as in most other continuous sample introduction systems. Ausserer et al 24 reported a sample introduction system with a capillary probe attached to a microfluidic chip for sampling from a 96-well plate. In their system, however, the sampling probe was attached perpendicularly to the plane of the chip for sampling from the well plate placed beneath the chip.…”

Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 99%

“…Ausserer et al reported the use of a commercial auto-sampler in connection with a chip-based CE system for achieving automated sample presentation. 24 Although effective, the approach also implies significant additional cost as well as a departure from the general aim for miniaturization of equipment.…”

Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 99%

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An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis

Fang

et al. 2005

Analyst

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An automated and continuous sample introduction system for microfluidic chip-based capillary electrophoresis (CE) was developed in this work. An efficient world-to-chip interface for chip-based CE separation was produced by horizontally connecting a Z-shaped fused silica capillary sampling probe to the sample loading channel of a crossed-channel chip. The sample presentation system was composed of an array of bottom-slotted sample vials filled alternately with samples and working electrolyte, horizontally positioned on a programmable linearly moving platform. On moving the array from one vial to the next, and scanning the probe, which was fixed with a platinum electrode on its tip, through the slots of the vials, a series of samples, each followed by a flow of working electrolyte was continuously introduced electrokinetically from the off-chip vials into the sample loading channel of the chip. The performance of the system was demonstrated in the separation and determination of FITC-labeled arginine and phenylalanine with LIF detection, by continuously introducing a train of different samples. Employing 4.5 kV sampling voltage (1000 V cm(-1) field strength) for 30 s and 1.8 kV separation voltage (400 V cm(-1) field strength) for 70 s, throughputs of 36 h(-1) were achieved with <1.0% carryover and 4.6, 3.2 and 4.0% RSD for arginine, FITC and phenylalanine, respectively (n = 11). Net sample consumption was only 240 nL for each sample.

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Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 99%

Section: Design Of the Automated Sample Introduction Systemmentioning

confidence: 99%

See 2 more Smart Citations

An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis

Fang

et al. 2005

Analyst

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“…Later, a similar strategy was used by other groups with microfabricated chipbased CE system, exploiting the differences in volume flow resistance of the sample introduction channel (SIC) and the sample loading channel (SLC) of the CE chip ( Fig. 1b) to achieve on-chip continuous sample introduction [8,9,10]. In chip-based systems, the SIC is used as a flow-through sample reservoir that is connected to the SLC, and not directly to the CE separation channel as in the H-channel design.…”

mentioning

confidence: 99%

Sample introduction for microfluidic systems

Fang

2004

Analytical and Bioanalytical Chemistry

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A High-Throughput Continuous Sample Introduction Interface for Microfluidic Chip-based Capillary Electrophoresis Systems

Fang

2002

Anal. Chem.

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The development of efficient sample introduction and pretreatment systems for microfluidic chip-based analytical systems is important for their application to real-life samples. In this work, world-to-chip interfacing was achieved by a novel flow-through sampling reservoir featuring a guided overflow design. The flow-through reservoir was fabricated on a 30 x 60 x 3 mm planar glass chip of crossed-channel design used for capillary electrophoresis separations. The 20-microL sample reservoir was produced from a section of plastic pipet tip and fixed at one end of the sampling channel. Sample change was performed by pumping 80-microL samples sandwiched between air segments at approximately 0.48 mL/min flow rate through the flow-through reservoir, introduced from an access hole on the bottom side of the chip. A filter paper collar wrapped tightly around the reservoir guided the overflowing sample solution into a plastic trough surrounding the reservoir and then to waste. The performance of the system was demonstrated in the separation and determination of FITC-labeled arginine, glycine, phenylalanine, and glutamic acid with LIF detection, by continuously introducing a train of different samples through the system without electrical interruption. Employing a separation channel of 4 cm (2-cm effective separation length) and 1.4-kV separation voltage, maximum throughputs of 80/h were achieved with <4.1% carryover and precisions ranging from 1.5% for arginine to 2.6% RSD (n = 11) for glycine. The sampling system was tested in the continuous monitoring of the derivatizing process of amino acids by FITC over a period of 4 h, involving 166 analytical cycles. An outstanding overall precision of 4.8% RSD (n = 166) was achieved for the fluorescein internal standard.

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Automated Lab-on-a-Chip Analysis of DNA Fragments

Cited by 4 publications

References 6 publications

An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis

An automated electrokinetic continuous sample introduction system for microfluidic chip-based capillary electrophoresis

Sample introduction for microfluidic systems

A High-Throughput Continuous Sample Introduction Interface for Microfluidic Chip-based Capillary Electrophoresis Systems

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