Automated measurement of spectral sensitivity of motion vision during optokinetic behavior

Kretschmer, Friedrich; Ahlers, Malte T.; Ammermüller, Josef; Kretzberg, Jutta

doi:10.1016/j.neucom.2011.12.026

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…The pattern is rotated around the animal at a constant velocity [1] in one [1, 5] or alternating directions [7]. Alternatively the velocity can be changed sinusoidally [8, 9]. These patterns can be painted or projected onto the inner surface of card board, plastic or wood cylinders as previously described for OKR or OMR experiments in mice [4, 10, 11, 12] and other species [13, 14], generating a complete 360° image at a homogeneous distance from the animal.…”

Section: Introductionmentioning

confidence: 99%

“…Thus the tested spatial frequencies vary during the experiment. Digital presentation techniques (with projectors [16, 9] or monitors [1, 17, 18, 7]) significantly speed up experiments by allowing the presentation of a large array of stimuli in quick succession. By simulating a virtual cylinder a constant grating can be presented in the field of view of the animal.…”

Section: Introductionmentioning

confidence: 99%

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A system to measure the Optokinetic and Optomotor response in mice

Kretschmer

Sajgo

Kretschmer

et al. 2015

Journal of Neuroscience Methods

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114

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Background Visually evoked compensatory head movements (Optomotor responses) or eye movements (Optokinetic responses) are extensively used in experimental mouse models for developmental defects, pathological conditions, and testing the efficacy of therapeutic manipulations. New Method We present an automated system to measure Optomotor and Optokinetic responses under identical stimulation conditions, enabling a direct comparison of the two reflexes. A semi-automated calibration procedure and a commercial eye tracker are used to record angular eye velocity in the restrained animal. Novel video tracking algorithms determine the location of the mouse head in real time and allow repositioning of the stimulus relative to the mouse head. Results Optomotor and Optokinetic responses yield comparable results with respect to determining visual acuity in mice. Our new head tracking algorithms enable a far more accurate analysis of head angle determination, and reveal individual head retractions, analogous to saccadic eye movements observed during Optokinetic Nystagmus. Comparison with Existing Methods To our knowledge this is the first apparatus allowing the direct comparison of Optomotor and Optokinetic responses in mice. Our tracking algorithms, which allow an objective determination of head movements are a significant increment over existing systems which rely on subjective human observation. The increased accuracy of the novel algorithms increases the robustness of automated Optomotor response determinations and reveals novel aspects of this reflex. Conclusions We provide the blueprints for inexpensive hardware, and release open source software for our system, and describe an accurate and accessible method for Optomotor and Optokinetic response determination in mice.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A system to measure the Optokinetic and Optomotor response in mice

Kretschmer

Sajgo

Kretschmer

et al. 2015

Journal of Neuroscience Methods

Self Cite

114

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show abstract

“…All three algorithms presented in this paper were successfully used to track the head gaze of animals performing optokinetic head movements [6], [7]. They are fast enough to perform online tracking on a common PC (Intel Pentium 4, 3 GHz, 4 GB RAM) at a resolution of 1280 1024 and 25 fps in a typical experimental setting.…”

Section: Discussionmentioning

confidence: 99%

“…The tracking of a triangle shaped marker was used in a preceding study [7] to determine the spectral sensitivity of turtle motion vision. …”

Section: A Tracking a Triangle Shaped Markermentioning

confidence: 99%

“…The third algorithm uses knowledge about the body shape of rodents to track the gaze without artificial makers. Preceding versions of the algorithms have successfully been applied in previous studies [6], [7] in the context of optokinetic response measurements of mice and turtles. All of these algorithms outperform manual tracking of gaze in precision and speed.…”

Section: Introductionmentioning

confidence: 99%

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Automated determinination of head gaze in rodents

Kretschmer

Kopcke

et al. 2012

2012 5th International Congress on Image and Signal Processing

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Behavioral experiments to determine the sensory performance of mice or rats often require the measurement of the animal's head gaze. Nevertheless, commercial systems for video tracking of laboratory animals are often restricted to tracking of the body position and sometimes the nose position, leading to defective estimates of the gaze direction or require several artificial markers. Most experimenters rely on subjective judgments of this important experimental measurement. In this study we present three algorithms to determine the exact gaze of freely moving rodents. Two of these algorithms rely on artificial markers which are attached to the animal's head. One approach uses knowledge about the body shape of rodents to track gaze without the use of artificial markers. All three algorithms determine the direction of gaze more precisely than a human observer and avoid systematic errors occurring in previously available tracking algorithms.Abstract-animal behavior; head tracking algorithms; mouse vision

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Ancestral photoreceptor diversity as the basis of visual behaviour

Baden

2024

Nat Ecol Evol

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Automated measurement of spectral sensitivity of motion vision during optokinetic behavior

Cited by 4 publications

References 28 publications

A system to measure the Optokinetic and Optomotor response in mice

A system to measure the Optokinetic and Optomotor response in mice

Automated determinination of head gaze in rodents

Ancestral photoreceptor diversity as the basis of visual behaviour

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