Automated metabolic profiling analysis of urinary steroids by a gas chromatography mass spectrometry data system

Vrbanac, J. J.; Sweeley, Charles C.; Pinkston, J. David

doi:10.1002/bms.1200100309

Cited by 12 publications

(1 citation statement)

References 13 publications

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“…Urine steroid profile analysis was performed according to methods previously reported [2,3,[6][7][8][9][10]. In brief, the method consisted of enzymatic hydrolysis of 0.5-2 ml of urine sample, extraction, derivative formation, purification, GC/MS-SIM analysis and quantification.…”

Section: Methodsmentioning

confidence: 99%

Reference Values for Urinary Steroids in Japanese Newborn Infants: Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring

et al. 2003

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Abstract. Urinary steroid profile analysis using gas chromatography/mass spectrometry (GC/MS) has been reported for the diagnosis of abnormal steroidogenesis in newborn infants with some success. We tried to establish the reference values of 63 urinary steroids in Japanese newborn infant, using GC/MS in selected ion monitoring (SIM) that utilizes two characteristic mass ions for each steroid for definitive identification. We studied 36 healthy full-term newborn infants (1-56 days of age) on spot urine samples to define the reference values (mg/g creatinine, median and 10-90 percentile range) and to investigate the possible difference between daytime and nighttime levels. We also studied 23 healthy adult females (20-24 years of age) on 24-hour-urine for the comparison of the reference values of newborn infants. Fifty metabolites of DHEA, pregnenolone, 17-hydroxypregnenolone, androstenedione, progesterone, 17-hydroxyprogesterone, 21-deoxycortisone, corticosterone, 18-hydroxycorticosterone, aldosterone, 18-hydroxycortisol, 11-deoxycortisol, cortisone, cortisol, and estrogen in each infant were measurable without interference, but 13 metabolites of 11-hydroxyandrostenedione, pregnenolone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 21-deoxycortisol, 11-deoxycortisol and cortisol were unmeasurable in each infant due to the interference of fetal cortex steroids as confirmed by abnormal peak area ratios of two mass ions. All 63 metabolites in each control adult were measurable without interference. 16a-, 16b-, and 15b-hydroxy metabolites of 3b-hydroxy-5-en-steroids, and 6b-, 18-hydroxy and 11-oxo-metabolites of corticosteroids were significantly higher in full-term newborn infants than those in adults as previously reported. Urinary steroids showed little circadian variation in the newborn infants, indicating that spot urine can substitute for 24-hour urine.

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Section: Methodsmentioning

confidence: 99%

Reference Values for Urinary Steroids in Japanese Newborn Infants: Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring

et al. 2003

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Automated profiling of urinary organic acids by dual-column gas chromatography and gas chromatography/mass spectrometry

Lefevere¹,

Verhaeghe²,

Declerck³

et al. 1988

Biol. Mass Spectrom.

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A multi-stage screening and identification scheme for the diagnosis of inborn errors in amino acid, fatty acid, carbohydrate and intermediary metabolism is described. The method is based on a computerized analysis of data obtained with dual-column gas chromatography/FID (GC/FID) and gas chromatography/mass spectrometry (GC/MS) profiling of urinary organic acids. It involves: (1) isolation of the compounds by solvent or solid-phase extraction; (2) conversion into trimethylsilyl (TMS) or TMS-oxime derivatives; (3) GC/FID analysis on SE-52 and OV-1701 capillary columns; (4) tentative identification by comparing the methylene unit (MU) values on both columns with a user-built library of reference compounds; (5) quantitative evaluation of the excretion profile; and (6) analysis by GC/MS of samples with an abnormal profile using an automated peak identification programme.

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A Study of the Human Urinary Steroid Metabolic Profile Using A DB‐1 Fused Silica Capillary Column

Sye

Cheng

1985

J Chinese Chemical Soc

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This paper reports the performance of a DB‐1 fused‐silica capillary column for analysis of mixtures of methoxime‐trimethylsilyl ether (MO‐TMS) derivatives of human urinary steroid extracts. DB‐1 is a non‐extractable bonded phase, equivalent in polarity to SE‐30 and OV‐1. In addition, a small reversed‐phase Sep‐Pak C18 cartridge is used for extraction of steroid conjugates from urine, and for the extraction of freed steroids liberated by hydrolysis. Examples of urinary steroid analysis in normal adult males and in patients with multiple myeloma using the DB‐1 bonded phase fused silica capillary column are described.

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Automated metabolic profiling analysis of urinary steroids by a gas chromatography mass spectrometry data system

Cited by 12 publications

References 13 publications

Reference Values for Urinary Steroids in Japanese Newborn Infants: Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring

Reference Values for Urinary Steroids in Japanese Newborn Infants: Gas Chromatography/Mass Spectrometry in Selected Ion Monitoring

Automated profiling of urinary organic acids by dual-column gas chromatography and gas chromatography/mass spectrometry

A Study of the Human Urinary Steroid Metabolic Profile Using A DB‐1 Fused Silica Capillary Column

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