Automated On-Line GPC-GC-FPD Involving Co-Solvent Trapping and the On-Column Interface for the Determination of Organophosphorus Pesticides in Olive Oils

Jongenotter, G. A.; Kerkhoff, Mia A.T.; Knaap, Henk C. M. van der; Vandeginste, B.G.M.

doi:10.1002/(sici)1521-4168(19990101)22:1<17::aid-jhrc17>3.3.co;2-z

Cited by 8 publications

(15 citation statements)

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“…Based on published data [1,2,3,4,5,6,7,8,9,10,11], we divided the pesticides used in olive growth into two groups. The first included pesticides that could be analyzed by gas chromatography and the second those that could be analyzed by liquid chromatography.…”

Section: Resultsmentioning

confidence: 99%

“…Pesticide use is subject to strict regulations, especially concerning residual levels in commercial foods (olives and olive oil). Several methods [1,2,3,4,5,6,7,8,9,10,11] have been developed for the analysis of pesticide residues in olive oil. These methods generally rely on liquid-liquid or liquid-solid extraction procedures, cleanup on a column and chromatographic separation techniques coupled with mass spectrometric detection, nitrogen-phosphorus detection (NPD) and flame photometric detection (FPD).…”

Section: Introductionmentioning

confidence: 99%

“…Besides, Jongenotter and al. [11] have used an on-line SEC-GC method to determine two organophosphorus pesticides in olive oil with FPD detection mode.…”

Section: Introductionmentioning

confidence: 99%

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Determination of residual pesticides in olive oil by GC–MS and HPLC–MS after extraction by size-exclusion chromatography

2003

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This work describes the development of a method for analyzing pesticide residues in olive oil by GC-MS and HPLC-MS. Pesticides were separated from the oily matrix by size-exclusion chromatography. After extraction, 20 pesticides were separated and analyzed by GC-MS and 11 others HPLC-MS in electrospray mode. The development of this method enabled us to identify and quantify the pesticides of interest.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of residual pesticides in olive oil by GC–MS and HPLC–MS after extraction by size-exclusion chromatography

2003

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“…Finally, the influence of the incubation time on the competition step (15,30,45, and 60 min) was investigated. I 50 and A 0 values increased gradually as time increased.…”

Section: Hapten Design and Synthesismentioning

confidence: 99%

Highly Sensitive Enzyme-Linked Immunosorbent Assay for Chlorpyrifos. Application to Olive Oil Analysis

Brun

Garcés-García

Puchades

et al. 2005

J. Agric. Food Chem.

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Highly sensitive enzyme-linked immunoassays for chlorpyrifos, one of the most applied insecticides, are presented. Several haptens were synthesized for immunoreagent production and ELISA development. The best immunoassays obtained are based on an indirect coated-plate immunoassay format. Two assays were optimized; one shows a limit of detection of 0.3 ng L(-1), an I50 of 271 ng L(-1), and a dynamic range between 4 and 16 474 ng L(-1). The other one has a limit of detection of 0.07 ng L(-1), an I50 of 7 ng L(-1), and a dynamic range between 0.4 and 302 ng L(-1). The assays were used to quantify chlorpyrifos in olive oil using a simple and rapid sample extraction procedure. The good recoveries achieved in both assays (109% mean value) and the agreement with values given by the GC reference method (110% mean value) indicate their potential for either screening or laboratory quantification.

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“…Clean up with gel permeation chromatography (17,20,21), size exclusion chromatography (14), other extraction methods involving solid-phase microextraction (9), and matrix solid-phase dispersion (11,19) have also been effectively developed. These methods are specific to well-studied pesticide groups and/or demand certain instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Multiresidue Method for Determination of 35 Pesticides in Virgin Olive Oil by Using Liquid−Liquid Extraction Techniques Coupled with Solid-Phase Extraction Clean Up and Gas Chromatography with Nitrogen Phosphorus Detection and Electron Capture Detection

Amvrazi

Albanis

2006

J. Agric. Food Chem.

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A method for the multiresidue determination of 35 pesticides (30 insecticides and five herbicides) in olive oil by gas chromatography (GC) is described. Three liquid-liquid extraction (LLE) procedures based on (i) partition of pesticides between acetonitrile (ACN) and oil solution in n-hexane, (ii) partition of pesticides between saturated ACN with n-hexane and oil solution in n-hexane saturated with ACN, and (iii) partition of pesticides between ACN and oil were tested for the optimization of the highest pesticide recoveries with the lowest oil residue in the final extracts. Experimental tests were preformed in order to study the efficiency of different clean up procedures with N-Alumina, Florisil, C18, and ENVI-Carb solid-phase extraction (SPE) cartridges for the compounds analyzed by GC-nitrogen phosphorus detection. A second step of clean up was also performed for the compounds analyzed by GC-electron capture detection (ECD), by using phenyl-bonded silica (Ph), diol-bonded silica (Diol), cyanopropyl-bonded silica (CN), and amino propyl-bonded silica (NH2) SPE cartridges. LLE of the oil solution in hexane with ACN followed by an ENVI-Carb SPE clean up of the extract gave the best results for all target compounds. The ACN extract was additionally cleaned through a Diol-SPE cartridge for the determination of pesticides analyzed mainly by GC-ECD. Pesticide recoveries form virgin olive oil spiked with 20, 100, and 500 microg/kg concentrations of pesticides ranged from 70.9 to 107.4%. The proposed method featured good sensitivity, pesticide quantification limits were low enough, and the precision, expressed as relative standard deviation, ranged from 2.4 to 12.0%. The proposed method was applied successfully for the residue determination of the selected pesticides in commercial olive oil samples.

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Automated On-Line GPC-GC-FPD Involving Co-Solvent Trapping and the On-Column Interface for the Determination of Organophosphorus Pesticides in Olive Oils

Cited by 8 publications

References 0 publications

Determination of residual pesticides in olive oil by GC–MS and HPLC–MS after extraction by size-exclusion chromatography

Determination of residual pesticides in olive oil by GC–MS and HPLC–MS after extraction by size-exclusion chromatography

Highly Sensitive Enzyme-Linked Immunosorbent Assay for Chlorpyrifos. Application to Olive Oil Analysis

Multiresidue Method for Determination of 35 Pesticides in Virgin Olive Oil by Using Liquid−Liquid Extraction Techniques Coupled with Solid-Phase Extraction Clean Up and Gas Chromatography with Nitrogen Phosphorus Detection and Electron Capture Detection

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