Automated sample treatment with the injection of large sample volumes for the determination of contaminants and metabolites in urine

Rodríguez‐Gonzalo, Encarnación; García-Gómez, Diego; Herrero‐Hernández, Eliseo; Carabias‐Martínez, Rita

doi:10.1002/jssc.201000202

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Blood, urine, breast milk, hair, and meconium are the most used specimens for biomonitoring. The development of automated offline and online methods increases the demand for high-throughput analysis in biomonitoring programs (Rodríguez-Gonzalo et al, 2010). The scientific community is very much aware of the toxic effect of endosulfan, therefore, this article highlights the toxic effect of endosulfan in the next section that is used in agriculture frequently with special emphasis on its biomonitoring in humans which is very essential to protect farmers and other fauna from the hazardous effect of endosulfan.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Biomonitoring of endosulfan toxicity in human

KARN¹,

Upadhyay²,

KUMAR³

2022

Biocell

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Chemicals are comprehensively used worldwide to control herbs, weeds, pests, and other competing agents with various growing crops. The consumption of crops grown with these chemicals (even in small quantities) can upshot into accumulation in the human body. People can accidentally inhale these hazardous chemicals if they are in an area where they were applied. These chemicals can be ingested in a human with contaminated food and drinks. Ultimately it causes various adverse effects (chronic toxicity, teratogenic, mutagenic, carcinogenic effect, reproductive, and organ toxicity) on human health. Among the pool of these chemicals used as pesticides in the environment, exposure of endosulfan to humans has a potential health risk throughout the globe. The poisoning of endosulfan is reported frequently in society, therefore, this article highlights the toxicological and other harmful properties of endosulfan to humans and emphasized its essential biomonitoring. Its quick monitoring and effective hazard evaluation are possible with multiomics technologies and some other analytical approaches. This review summarizes the introduction and mechanism of action of endosulfan along with some other toxic chemicals used in the agriculture work, the toxicity of endosulfan in human/environment, and importantly its biomonitoring (detection of chemicals and their metabolites in a biological sample) in human because biomonitoring is often considered as the most preferred method. Biomonitoring could be easily done in human samples (blood, hair, milk, and urine) with a multi-omics approach, which is a quick, reliable, and state-of-the-art technique.

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Section: Mechanism Of Actionmentioning

confidence: 99%

Biomonitoring of endosulfan toxicity in human

KARN¹,

Upadhyay²,

KUMAR³

2022

Biocell

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“…It was also the first commercially produced RAM to be introduced into the market (LiChrospher ADS by Merck, Darmstadt, Germany). Through the years, ADS RAM have been used in different situations, such as analysis of drug compounds from plasma [48,62], serum [63,64], bronchial fluids [65], cell lysates [48], hepatocyte extracts, and urine [62,[65][66][67][68][69][70].…”

Section: Adsmentioning

confidence: 99%

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Yang

Fan

Classon³

et al. 2013

J of Separation Science

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Restricted access media (RAM) as an alternative to traditional sample preparation strategies are reviewed. RAM comprise chromatographic packing materials that combine, typically, a restrictive outer surface to exclude the retention of large biomolecules, which are common interferences in biological fluids, with retentive inner pores or phases to capture analytes of interest. Through the years, a variety of RAM formats have been created, including internal surface phases, semipermeable phases, and molecularly imprinted polymer phases. Many phases are commercially available through a variety of manufacturers. The use of on-line sample preparation using RAM can increase throughput, recovery, and ease of use for sample preparation of complex biological matrices. The state-of-the-art with respect to production and use of these media for a variety of applications is covered.

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“…Large-volume injection liquid chromatography and mass spectrometry has been used for the determination of contaminants and metabolites in urine 32 , as well as illicit drugs 6, 33 , and steroids 11 , in municipal wastewater influent. Large-volume injection was chosen to minimize sample preparation, and to maximize sensitivity needed for detection and quantification.…”

Section: Introductionmentioning

confidence: 99%

“…The objective of this study is to develop a method using large-volume injection, liquid chromatography, mass spectrometry to quantify nucleosides and modied nucleosides in municipal wastewater inuent. Large-volume injection liquid chromatography and mass spectrometry has been used for the determination of contaminants and metabolites in urine, 32 as well as illicit drugs, 6,33 and steroids, 11 in municipal wastewater inuent. Large-volume injection was chosen to minimize sample preparation, and to maximize sensitivity needed for detection and quantication.…”

Section: Introductionmentioning

confidence: 99%

Analysis of nucleosides in municipal wastewater by large-volume liquid chromatography tandem mass spectrometry

Brewer

Lunte

2015

Anal. Methods

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Nucleosides are components of both DNA and RNA, and contain either a ribose (RNA) or 2deoxyribose (DNA) sugar and a purine or pyrimidine base. In addition to DNA and RNA turnover, modified nucleosides found in urine have been correlated to a diminished health status associated with AIDS, cancers, oxidative stress and age. Nucleosides found in municipal wastewater influent are potentially useful markers of community health status, and as of now, remain uninvestigated. A method was developed to quantify nucleosides in municipal wastewater using large-volume injection, liquid chromatography, and mass spectrometry. Method accuracy ranged from 92 to 139% when quantified by using isotopically labeled internal standards. Precision ranged from 6.1 to 19% of the relative standard deviation. The method’s utility was demonstrated by the analysis of twenty-four hour composite wastewater influent samples that were collected over a week to investigate community nucleoside excretion. Nucleosides originating from RNA were more abundant that DNA over the study period, with total loads of nucleosides ranging from 2 to 25 kg/day. Given this relatively high amount of nucleosides found over the study period they present an attractive analyte for the investigation of community health.

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Automated sample treatment with the injection of large sample volumes for the determination of contaminants and metabolites in urine

Cited by 9 publications

References 27 publications

Biomonitoring of endosulfan toxicity in human

Biomonitoring of endosulfan toxicity in human

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Analysis of nucleosides in municipal wastewater by large-volume liquid chromatography tandem mass spectrometry

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