2001
DOI: 10.1016/s0021-9673(01)00900-1
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Automated signature peptide approach for proteomics

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Cited by 73 publications
(50 citation statements)
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“…One is for the enrichment of phosphorylated peptides with Ga(III) or Fe(III) immobilized [19]. The other is for the selection of peptides with Cu(II) loaded on to the columns [20][21][22][23].…”
Section: Introductionmentioning
confidence: 99%
“…One is for the enrichment of phosphorylated peptides with Ga(III) or Fe(III) immobilized [19]. The other is for the selection of peptides with Cu(II) loaded on to the columns [20][21][22][23].…”
Section: Introductionmentioning
confidence: 99%
“…However, reaction conditions have to be chosen carefully to avoid both incomplete esterification and side reactions for instance with asparagines because they increase sample complexity and interfere with subsequent mass spectrometric analysis. Thus, various metal-ions such as Fe 31 , Ga 31 , Al 31 or Zr 41 have been used for better selectivity and phosphopeptide recovery [26,27]. Especially Ga 31 -ions have proven well [28] in different studies but Fe 31 -based methods are still used more often.…”
Section: Detection Of Phosphoproteins In 2-d Gelsmentioning
confidence: 99%
“…Especially Ga 31 -ions have proven well [28] in different studies but Fe 31 -based methods are still used more often. The good compatibility of IMAC-procedures to subsequent separation and detection techniques such as CE [29], LC-MS/MS [30,31] and direct MALDI-MS of phosphopeptides bound to IMAC-beads on the target [32], will grant this technique even wider spreading in the future. Another possibility for decreasing the co-purification of acidic peptides is the use of TiO 2 -columns [33].…”
Section: Detection Of Phosphoproteins In 2-d Gelsmentioning
confidence: 99%
“…However, many techniques are used to extract or highlight peptides of choice. For example, immobilised metal ion affinity chromatography utilises chromatographic media with bound Ni ions to extract phosphopeptides from complex mixtures, thus enabling the identification of peptides from proteins involved in signalling cascades (Riggs et al 2001). Isotope-coded affinity tag (ICAT) methodologies use stable-isotope tags to differentially label two samples which can then be mixed.…”
Section: Chromatography-based Technologiesmentioning
confidence: 99%