Automated single nucleotide polymorphism typing using bead array in capillary tube

Kagawa, Yasuo; Hiraoka, Mami; Miyashita-Hatano, Yukiko; Shishido-Oki, Madoka; Yoshida, Mamiko; Kondou, Susumu; Sugiura, Mika; Sawakami-Kobayashi, Kazumi; Takahashi, Masaaki; Tajima, Hideji; Yohda, Masafumi

doi:10.1016/j.jbiosc.2010.05.007

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…DNA was extracted from whole blood using a Magtration System (Precision Systems Science Co. Ltd., Chiba, Japan) and magnetic beads [27]. To rapidly and inexpensively genotype a large number of blood samples, we developed an automated genotyping machine using a bead array in a capillary tube [28]. This BIST method specifically genotypes the C677T single-nucleotide polymorphism (rs1801133) in MTHFR using beads in a straw tip [28].…”

Section: Genotypingmentioning

confidence: 99%

“…To rapidly and inexpensively genotype a large number of blood samples, we developed an automated genotyping machine using a bead array in a capillary tube [28]. This BIST method specifically genotypes the C677T single-nucleotide polymorphism (rs1801133) in MTHFR using beads in a straw tip [28]. If necessary, DNA was amplified by polymerase chain reaction and analyzed by electrophoresis in a 10% polyacrylamide gel.…”

Section: Genotypingmentioning

confidence: 99%

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Nutritional Guidance in Sakado Folate Project

Yurimoto¹,

Hiraoka²,

Kageyama³

et al. 2018

B Group Vitamins - Current Uses and Perspectives

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Background: Serum folate levels are lower in TT homozygotes of the single-nucleotide polymorphism (rs1801133) of methylenetetrahydrofolate reductase (MTHFR) than in CC homozygotes and CT heterozygotes. Objective: To improve folate status, the genotype was notified to each subject to motivate them to eat more green-yellow vegetables. Design: Genotype, dietary folate intake, and blood biochemistry were determined and statistically analyzed for 404 subjects (109 males, mean 58.9 years; 295 females, mean 61.8 years). Their serum folate and total homocysteine (tHcy) concentrations were measured before and after receiving nutritional guidance and genotype notification. Results: The frequencies of the CC, CT, and TT MTHFR genotypes were 35.4, 49.7, and 14.8%, respectively. TT homozygote participants significantly increased their intake of green-yellow vegetables (p < 0.01) and of food-derived folate (p < 0.05) following nutritional guidance. The increase in serum folate (p < 0.001) and the decrease in tHcy (p < 0.001) in TT homozygotes following nutritional guidance were more than twice that of the CC homozygote and CT heterozygote participants. An increase in broccoli, spinach and Komatsuna intake was observed following nutritional guidance, irrespective of the season.

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Section: Genotypingmentioning

confidence: 99%

Section: Genotypingmentioning

confidence: 99%

Nutritional Guidance in Sakado Folate Project

Yurimoto¹,

Hiraoka²,

Kageyama³

et al. 2018

B Group Vitamins - Current Uses and Perspectives

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“…The chemiluminescence of each bead was then measured using a photodetection unit for BIST (BISTnner; Precision System Science Co., Ltd.). 12 The reaction beads showed a linear increase in chemiluminescence that was proportional to the TSH concentration (Fig. 2).…”

mentioning

confidence: 90%

Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization

et al. 2014

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“…While the technique was initially carried out by hand, the IoNS was involved in the invention of automatic gene analysis machine 10 years ago to overcome a shortage of technicians. 10 Today, the upgraded machine known as geneLEADVIII is involved in PCR analysis for COVID-19 diagnosis all over the world, and the company that manufactures the machine has received appreciation from the French government. In addition, the IoNS actively disseminates health and nutritional messages based on international recommendations, outcomes from cutting-edge research, and studies conducted by the IoNS through its Web site which is currently attracting the public’s attention.…”

Section: Introductionmentioning

confidence: 99%