Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry

Brown, Simon H. J.; Huxtable, Liam H.; Willcox, Mark; Blanksby, Stephen J.; Mitchell, Todd W.

doi:10.1039/c2an36189b

Cited by 26 publications

(26 citation statements)

References 21 publications

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“…Lipid profiles were compared to those obtained from human plasma and revealed that long chain polyunsaturated cholesterol esters and some SMs were significantly enhanced in plaques. LESA has also been successfully employed for the analysis of lipid deposits on worn contact lenses in an effort to understand the origins of contact lens discomfort and spoilage [231]. Lipid classes detected included PC, SM, cholesterol esters, wax esters and O-acyl--hydroxy fatty acids.…”

Section: Liquid Extraction Surface Analysis (Lesa)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-ofthe-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractMass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment;bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lip...

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Section: Liquid Extraction Surface Analysis (Lesa)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

Self Cite

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“…In contrast to other ambient analysis techniques mentioned above, the process of surface sampling and ionisation are decoupled, offering opportunities for superior quantification. Whilst LESA has shown promise for the profiling of proteins, lipids, drugs and metabolites in tissue and blood [10][11][12][13], its potential application to the chemical profiling of other biological fluids has not yet been considered.…”

Section: Introductionmentioning

confidence: 99%

“…Liquid Extraction Surface Analysis (LESA) [9][10][11][12][13] is a new tool in surface mass spectrometry, which also operates under ambient conditions and has recently become commercially available. The technique involves the deposition of a solvent droplet from a pipette tip onto a surface.…”

Section: Introductionmentioning

confidence: 99%

Analysis of urine, oral fluid and fingerprints by liquid extraction surface analysis coupled to high resolution MS and MS/MS – opportunities for forensic and biomedical science

et al. 2016

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Liquid Extraction Surface Analysis (LESA) is a new, high throughput tool for ambient mass spectrometry. A solvent droplet is deposited from a pipette tip onto a surface and maintains contact with both the surface and the pipette tip for a few seconds before being re-aspirated. The technique is particularly suited to the analysis of trace materials on surfaces due to its high sensitivity and low volume of sample removal. In this work, we assess the suitability of LESA for obtaining detailed chemical profiles of fingerprints, oral fluid and urine, which may be used in future for rapid medical diagnostics or metabolomics studies. We further show how LESA can be used to detect illicit drugs and their metabolites in urine, oral fluid and fingerprints. This makes LESA a potentially useful tool in the growing field of fingerprint chemical analysis, which is relevant not only to forensics but also to medical diagnostics. Finally, we show how LESA can be used to detect the explosive material RDX in contaminated artificial fingermarks.

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“…84 Such “contact” model LESA may be advantageous for the analysis of samples containing a protective layer on the surface. When installed on a fully automated robotic system, it enables high-throughput, spatially resolved analysis of metabolites, 85,86 lipids, 87–90 and proteins 91,92 in complex samples, including dried blood spots, 84,88 contact lenses, 89 tissues, 55,83,86,91 and microbial colonies. 84,93 LESA also has been used for analyzing molecules separated using TLC 83 and capillary electrophoresis.…”

Section: Ambient Liquid Extraction Techniquesmentioning

confidence: 99%