Efficient night-time vessel detection is of significant importance for maritime traffic management, fishery activity monitoring, and environmental protection. With the advancement in object-detection approaches, the method of night-time vessel detection has gradually shifted from traditional threshold segmentation to deep learning that balances efficiency and accuracy. However, the restricted spatial resolution of night-time light (NTL) remote sensing data (e.g., VIIRS/DNB images) results in fewer discernible features and insufficient training performance when detecting vessels that are considered small targets. To address this, we establish an Enhanced Dense Nested-Attention Network (DNA-net) to improve the detection of small vessel targets under low-light conditions. This approach effectively integrates the original VIIRS/DNB, spike median index (SMI), and spike height index (SHI) images to maintain deep-level features and enhance feature extraction. On this basis, we performed vessel detection based on the Enhanced DNA-net using VIIRS/DNB images of the Japan Sea, the South China Sea, and the Java Sea. It is noteworthy that the VIIRS Boat Detection (VBD) observations and the Automatic Identification System (AIS) data were cross-matched as the actual status of the vessels (VBD-AIS). The results show that the proposed Enhanced DNA-net achieves significant improvements in the evaluation metrics (e.g., IOU, Pd, Fa, and MPD) compared to the original DNA-net, achieving performance of 87.81%, 96.72%, 5.42%, and 0.36 Wpx, respectively. Meanwhile, we validated the detection performance of Enhanced DNA-net and strong VBD detection against VBD-AIS, showing that the Enhanced DNA-net achieves 1% better accuracy than strong VBD detection.