Pathology telemedicine uses digital data of pathological samples to acquire technical support from sophisticated remote experts, aiding cancer diagnosis in less developed regions. In this paper, we propose a color 3D observation system composed of three cameras and a digital projector, achieving complete range 3D imaging of pathology tissues. A new phase mapping method for the projector calibration is proposed by locating the geometric centroid of the checkboard’s white squares, avoiding the center positioning error of traditional circular markers introduced by perspective distortion. A denoising method successively using amplitude modulation judge, phase monotonicity check, and phase abruptness constraint is proposed, which facilitates obtaining clean 3D point clouds.