Automatic circuits for backing off the photodetector signal in kinetic spectrophotometry

Keene, J. P.; Bell, C.

doi:10.1016/0020-7055(73)90009-0

Cited by 28 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DC conductance method has the advantage of high time resolution-nanoseconds in non-polar solvents (Baxendale et al 1974b)-but with aqueous solutions the permissible range of p H and ionic strength is limited. The high conductivity of unirradiated aqueous solutions containing ionic solutes can be cancelled out by subtracting the conductance of a second, un-irradiated sample cell (Barker et a1 1970), or using the same circuits used to 'back-off' photomultiplier signals (Keene and Bell 1973, Baxendale et a1 1974b, Michaels et a1 1976. Maughan et a1 (1978) have used reversing transformers as a wide-band filter to eliminate the DC polarisation effects allowing conductivity measurements to be made in aqueous solutions with a time resolution of -5 ns.…”

Section: Detection Of Transients By Conductivity Changes or Polarographymentioning

confidence: 99%

Application of pulse radiolysis methods to study the reactions and structure of biomolecules

Wardman

1978

Rep. Prog. Phys.

View full text Add to dashboard Cite

T o study reactions occurring on timescales faster than N 10-&lo-3 s, simple mixing of reagents is too slow and other methods of initiating reactions have to be used. Pulses of thermal energy, light quanta or ionising radiation have been used to perturb chemical and biological systems from equilibrium. Pulse radiolysis is the more recent of these three methods of initiating fast reactions, and this review emphasises the versatility of this technique for studying events occurring between 10-11 and 1 0 2 s after energy absorption. The extent and tiniescale of radiation damage in dilute aqueous solutions are outlined and the facile manipulation of experimental conditions to isolate a single reactive species is illustrated. The basic requirements for the radiation pulse and detection system are discussed. T h e experimental techniques which have been used to monitor fast reactions following pulse radiolysis include optical absorption spectroscopy and light scattering, and timeresolved electrical conductivity, polarography, and electron spin resonance spectroscopy. Some applications of pulse radiolysis to study fast reactions of biologically important molecules are illustrated, including the kinetics and thermodynamics of redox processes and electron-transfer reactions, enzymic processes and structural effects which are revealed by physical or kinetic properties.

show abstract

Section: Detection Of Transients By Conductivity Changes or Polarographymentioning

confidence: 99%