Automatic delineation of brain regions on MRI and PET images from the pig

Villadsen, Jørgen; Hansen, Hanne D.; Jørgensen, Lise Bolt; Keller, Sune H.; Andersen, Flemming; Petersen, Ida Nymann; Knudsen, Gitte M.; Svarer, Claus

doi:10.1016/j.jneumeth.2017.11.008

Cited by 35 publications

(32 citation statements)

References 35 publications

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“…Images consisted of 207 planes of 256 × 256 voxels of 1.22 × 1.22 × 1.22 mm. A summed picture of all counts in the 150-min scan was reconstructed for each pig and used for co-registration to a standardized MRI-based atlas of the domestic pig brain, similar to that previously published [80]. The time activity curveds (TACs) were calculated for the following volumes of interest (VOIs): thalamus, striatum, hippocampus, cerebellum, white matter, frontal cortex, somatosensory cortex, occipital cortex, rest of the cortex.…”

Section: Determination Of Free Fractionmentioning

confidence: 99%

In Vitro and In Vivo Characterization of Dibenzothiophene Derivatives [125I]Iodo-ASEM and [18F]ASEM as Radiotracers of Homo- and Heteromeric α7 Nicotinic Acetylcholine Receptors

Donat

Hansen

et al. 2020

Molecules

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The α7 nicotinic acetylcholine receptor (α7 nAChR) is involved in several cognitive and physiologic processes; its expression levels and patterns change in neurologic and psychiatric diseases, such as schizophrenia and Alzheimer’s disease, which makes it a relevant drug target. Development of selective radioligands is important for defining binding properties and occupancy of novel molecules targeting the receptor. We tested the in vitro binding properties of [125I]Iodo-ASEM [(3-(1,4-diazabycyclo[3.2.2]nonan-4-yl)-6-(125I-iododibenzo[b,d]thiopentene 5,5-dioxide)] in the mouse, rat and pig brain using autoradiography. The in vivo binding properties of [18F]ASEM were investigated using positron emission tomography (PET) in the pig brain. [125I]Iodo-ASEM showed specific and displaceable high affinity (~1 nM) binding in mouse, rat, and pig brain. Binding pattern overlapped with [125I]α-bungarotoxin, specific binding was absent in α7 nAChR gene-deficient mice and binding was blocked by a range of α7 nAChR orthosteric modulators in an affinity-dependent order in the pig brain. Interestingly, relative to the wild-type, binding in β2 nAChR gene-deficient mice was lower for [125I]Iodo-ASEM (58% ± 2.7%) than [125I]α-bungarotoxin (23% ± 0.2%), potentially indicating different binding properties to heteromeric α7β2 nAChR. [18F]ASEM PET in the pig showed high brain uptake and reversible tracer kinetics with a similar spatial distribution as previously reported for α7 nAChR. Blocking with SSR-180,711 resulted in a significant decrease in [18F]ASEM binding. Our findings indicate that [125I]Iodo-ASEM allows sensitive and selective imaging of α7 nAChR in vitro, with better signal-to-noise ratio than previous tracers. Preliminary data of [18F]ASEM in the pig brain demonstrated principal suitable kinetic properties for in vivo quantification of α7 nAChR, comparable to previously published data.

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Section: Determination Of Free Fractionmentioning

confidence: 99%

In Vitro and In Vivo Characterization of Dibenzothiophene Derivatives [125I]Iodo-ASEM and [18F]ASEM as Radiotracers of Homo- and Heteromeric α7 Nicotinic Acetylcholine Receptors

Donat

Hansen

et al. 2020

Molecules

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“…Neuroanatomical atlases are essential tools for studying the brain and its organization. In the last decades, most of such atlases were published in the form of printed books or articles (Bjarkam et al., 2017; Felix et al., 1999; Kornum et al., 2010; Paxinos and Watson, 2007; Saikali et al., 2010; Villadsen et al., 2018; Watanabe et al., 2001). Those books were usually quite expensive and due to their large size, not very handy to use.…”

Section: Introductionmentioning

confidence: 99%

Online histological atlas of the Göttingen minipig brain

Orłowski

Glud

Palomero-Gallagher

et al. 2019

Heliyon

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BackgroundThe cytoarchitecture of the Göttingen minipig telencephalon has recently been elucidated in the published article (Bjarkam et al., 2017). The aim of the current paper is to describe how such data can be presented in an online histological atlas of the Gottingen minipig brain and how this atlas was constructed.MethodsTwo sets of histological sections were used. One set was photographed in high resolution and labelled, the other set in low resolution (resized first set) was used for reference on the computer screen. The two sets of microphotographs enable, using the freely available JQuery Image Zoom Plugin, the subsequent construction of a simple HTML-based atlas web page with a “virtual microscope like” style, which allowed magnifying of the base image (low-resolution image) up to the maximum resolution of the high-resolution image. In addition, we describe how the established histological atlas can be accompanied by a set of similar T1-weighted MRI pictures.Results and conclusionHistological and MRI pictures are presented in atlas form on www.cense.dk/minipig_atlas/index.html. The described pipeline represent a cheap and freely available way to present histological images, in online virtual microscopic atlas form, and may thus be of general interest to anyone who would like to present histological data accordingly.

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“…The main limitations of our population averaged brain template are the low subject number and the inclusion of only female subjects. We included 16 animals in generating the T2 brain template based on the observation that templates stabilize at around 10 subjects (Avants et al, 2014) and the numbers used in other similar studies (Zhong et al, 2016;Villadsen et al, 2018;Johnson et al, 2019); however, we recognize that many human brain templates include one hundred or more subjects, and larger numbers would increase our confidence that our template is representative of the population. All the animals used in our study were female as we work exclusively with female micropigs.…”

Section: Limitationsmentioning

confidence: 99%