Automatic detecting and counting magnetic beads‐labeled target cells from a suspension in a microfluidic chip

Song, Zhenyu; Li, Mengqi; Li, Bao; Yan, Yimo; Song, Yongxin

doi:10.1002/elps.201800345

Cited by 15 publications

(8 citation statements)

References 62 publications

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“…When integrated into microfluidic systems, this 1950's technology is finding new applications. [5][6][7][8][9] New manufacturing processes, coupled with improved electronics have enabled them to characterise analytes' sizes [10][11][12] , concentrations 13,14 , shapes 15 and charges. 16,17 As a result, RPS has found numerous applications within environmental monitoring of, for example: bacteria 18 , algae 19 , and heavy metal ions 20 .…”

Section: Introductionmentioning

confidence: 99%

A Tunable Three-Dimensional Printed Microfluidic Resistive Pulse Sensor for the Characterization of Algae and Microplastics

2020

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Technologies that can detect and characterise particulates in liquids have applications in health, food and environmental monitoring. Simply counting the numbers of cells or particles however is not sufficient for most applications, and other physical properties must also be measured. Typically, it is necessary to compromise between chemical and biological specificity of the sensor and its speed. Here we present a low-cost and high-throughput multiuse counter that classifies a particle's size, concentration, porosity and shape. Using an additive manufacturing process, we have assembled a reusable flow resistive pulse sensor capable of being be tuned in real time to measure particles from 2-30 m, across a range of salt concentrations i.e. 2.5 × 10-4 to 0.1M. The device remains stable for several days with repeat measurements. We demonstrate its use for characterising algae with spherical and rod structures as well as microplastics shed from teabags. We present a methodology that results in a specific signal for microplastics, namely a conductive pulse, in contrast to particles with smooth surfaces such as calibration particles or algae, allowing the presence of microplastics to be easily confirmed and quantified. In addition, the shape of the signal and particle are correlated, giving an extra physical property to characterise suspended particulates. The technology can rapidly screen volumes of liquid, 1 mL/ min, for the presence of microplastics and algae.

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Section: Introductionmentioning

confidence: 99%

A Tunable Three-Dimensional Printed Microfluidic Resistive Pulse Sensor for the Characterization of Algae and Microplastics

2020

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“…Recent advances in the RPS sensors have utilised pores of different aspect ratios/shapes or specific electrode configur-ations to allow larger pores and sensing zones to be utilised. [21][22][23][24] When integrated into microfluidic systems this technology is further advanced, [25][26][27][28][29] allowing users to combine sample handling within the analytical system. A variety of innovations in this area, summarised in reviews elsewhere, 30,31 have focused on different forms of fabrication, convection and applications.…”

Section: Introductionmentioning

confidence: 99%

Multi-resistive pulse sensor microfluidic device

Pollard

Maugi

Platt

2022

Analyst

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“…Recent advances in the RPS sensors have utilised pores of different aspect ratios/shapes or specific electrode configurations to allow utilise larger pores and sensing zones. [21][22][23][24] When integrated into microfluidic systems this technology is further advanced, [25][26][27][28][29] allowing users to combine sample handling within the analytical system. A variety of innovations in this area, summarised in reviews elsewhere, 30,31 have focused on different forms of fabrication, convection and applications.…”

Section: Introductionmentioning

confidence: 99%

Universal Nanopore Platform Integrating Multiple Resistive Pulse Sensors into a Single Microfluidic Device

Pollard

Maugi

Holzinger

et al. 2021

Preprint

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Resistive pulse sensors have been used to characterise everything from whole cells to small molecules. Their integration into microfluidic devices have simplified sample handling whilst increasing throughput. Typically, these devices measure a limited size range or a specific analyte, making them prone to blockages in complex sample matrixes. To prolong their life and facilitate their use, samples are often filtered or prepared to match the sample with the sensor diameter. Here, we advance our tuneable flow resistive pulse sensor which utilises additively manufactured parts. The sensor allows parts to be easily changed, washed and cleaned, its simplicity and versatility allows components from existing nanopore fabrication techniques such as silicon nitride, polyurethane and glass pipettes to be integrated into a single device. This creates a multi-nanopore sensor that can simultaneously measure particles from 0.1 to 30 m in diameter. The orientation and controlled fluid flow in the device allows the sensors to be placed in series, whereby smaller particles can be measured in the presence of larger ones without the risk of being blocked. We demonstrate the device with a range of nanopore materials commonly found within the literature, the easiest to set up was the pulled glass pipette and glass nanopore membrane. However, the glass nanopore membrane was by far the most robust and reusable component tested. We illustrate the concept of a multi-pore flow resistive pulse sensor, by combining an additively manufactured tuneable sensor, termed sensor 1, with a fixed nanopore sensor, termed sensor 2. Sensor 1 measures particles 2 to 30 m in diameter, whilst sensor 2 can be used to characterise particles as small as 100 nm, depending upon its dimensions.

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Automatic detecting and counting magnetic beads‐labeled target cells from a suspension in a microfluidic chip

Cited by 15 publications

References 62 publications

A Tunable Three-Dimensional Printed Microfluidic Resistive Pulse Sensor for the Characterization of Algae and Microplastics

A Tunable Three-Dimensional Printed Microfluidic Resistive Pulse Sensor for the Characterization of Algae and Microplastics

Multi-resistive pulse sensor microfluidic device

Universal Nanopore Platform Integrating Multiple Resistive Pulse Sensors into a Single Microfluidic Device

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