Automatic Measurements of Diameter, Distension and Intima Media Thickness of the Aorta in Premature Rabbit Pups Using B-Mode Images

Nilsson, Tobias; Segstedt, Simon; Milton, Patrik; Sveinsdóttir, Snjolaug; Jansson, Tomas; Persson, Henry; Ley, David; Cinthio, Magnus

doi:10.1016/j.ultrasmedbio.2013.09.017

Cited by 10 publications

(5 citation statements)

References 20 publications

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“…The image data were transferred to a PC for post‐processing. Longitudinal motion of the intima‐media complex, and, when possible (see below) of the adventitial region, as well as the minimum lumen diameter (at end‐diastole), distension and intima‐media thickness (IMT) were automatically measured using an in‐house developed image analysis software based on Nilsson et al, 2013 and Nilsson et al, 2014 using MATLAB (The MathWorks Inc.). To measure intramural shear strain of the arterial wall, the longitudinal motion was measured in both the intima‐media and the adventitial regions, respectively, and the intramural shear strain angle (γ) was calculated using

where

and LMov is longitudinal movement, IM is intima‐media, Adv is adventitia, ed is end diastole, and d the radial distance between the two measurement positions (see also Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Response of the carotid artery longitudinal motion to submaximal physical activity in healthy humans—Marked changes already at low workload

Ahlgren

Erlöv

Cinthio

2023

Physiological Reports

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Until about 20 years ago the longitudinal motion of the arterial wall has been assumed to be negligible in comparison with the diameter change (Nichols et al., 2005). We and others have, however, shown that the arterial wall of large arteries at rest do not only show a well-known diameter change during the cardiac cycle, but also shows a distinct longitudinal displacement, that is, a motion along the artery parallel to blood flow, of the same magnitude as the diameter change (

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where

and LMov is longitudinal movement, IM is intima‐media, Adv is adventitia, ed is end diastole, and d the radial distance between the two measurement positions (see also Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

Response of the carotid artery longitudinal motion to submaximal physical activity in healthy humans—Marked changes already at low workload

Ahlgren

Erlöv

Cinthio

2023

Physiological Reports

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“…Disease was not homogeneous along the aorta of the rabbits studied. Although animal model studies may be recommended for certain investigations, 6 an animal model was not mandatory for this type of study. The major advantage was the practicality and availability of the model for an expeditious study as a function of the ongoing atherosclerotic treatment research.…”

Section: Discussionmentioning

confidence: 99%

Technology Dependence of Intima-Media Thickness Ultrasonographic Measurements

Braga¹,

Salles-Cunha²,

Barros

et al. 2017

Journal for Vascular Ultrasound

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Introduction Ultrasonographic (US) measurements of intima-media thickness (IMT), particularly of the carotid arteries, have been used as a cardiovascular risk assessment tool. Variations in manual and semiautomatic measurements were investigated in an animal model—the aorta of 10 rabbits with dyslipedemia. Methods IMT was measured in B-mode images with 256 shades of gray using a 10 MHz probe by two independent investigators: (a) three same-place manual measurements were averaged in five distinct locations of the aorta of each rabbit for a total of 50 measurements per investigator (subgroups M1 and M2); (b) experimental software allowed for alterations in blood-intima and media-adventitia brightness line definition for IMT detection; observer 1 used brightness 50–200, adaptable to all images (SA1); observer 2 started with 10–60—the usual for human carotid measurements in this package—and increased values until IMT lines were visually acceptable; IMT was measured at optimized values for each image (SA2). Mean values were compared by using the paired t-test available in Excel. Results SA1 measurements, 0.379 ± 0.112 (SD) mm, were significantly smaller than SA2, 0.403 ± 0.122 mm, M1, 0.404 ± 0.149 mm, and M2, 0.405 ± 0.140 mm (<0.001 for all three SA1 versus SA2, M1 and M2 comparisons). Conclusion/Discussion Distinct automatic technologies, including (a) selection of blood-intima and media-adventitia brightness interfaces and (b) signal processing to “improve” line images, may alter IMT measurements significantly. Assumed population variations may be affected by technology used. IMT gender and age tables may be applicable only if the same measurement technology was used consistently.

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“…The ultrasound cine loops, along with pressure profiles, were analyzed using an in‐house developed software. The software estimated the mean pulse pressure, distension (Nilsson et al, 2014 ), and longitudinal displacement (Albinsson et al, 2014 ) of four pressure cycles in each sequence.…”

Section: Methodsmentioning

confidence: 99%

“…In the older subjects in (c) and (d), the first antegrade motion is preceded by a distinct retrograde motion. The longitudinal motion and diameter change were measured using the methods of Nilsson et al ( 2013 and 2014 ), respectively. The curves shown are an average of curves from four to six cardiac cycles.…”

Section: Introductionmentioning

confidence: 99%

The impact of geometry, intramural friction, and pressure on the antegrade longitudinal motion of the arterial wall: A phantom and finite element study

Sjöstrand

Widerstrom

Svensson

et al. 2023

Physiological Reports

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Longitudinal motion of the carotid arterial wall, as measured with ultrasound, has shown promise as an indicator of vascular health. The underlying mechanisms are however not fully understood. We have found, in in vivo studies, that blood pressure has a strong relation to the antegrade longitudinal displacement in early systole. Further, we have identified that a tapered geometry and the intramural friction in-between two parts of a vessel wall influence the longitudinal displacement. We therefore studied the interaction between pressure, vessel geometry and intramural friction, tapered and straight ultrasound phantoms in a paralleled hydraulic bench study and corresponding numerical models. Profound antegrade longitudinal motion was induced in the innermost part of both tapered phantoms and the numerical models, but to a lesser extent when intramural friction was increased in the simulations. Strong correlations (R = 0.82-0.96; p < 1e-3; k = 9.3-14 μm/mmHg) between longitudinal displacement and pulse pressure were found in six of seven regions of interest in tapered phantoms. The motion of the straight phantom and the corresponding numerical model was smaller, on average zero or close to zero. This study demonstrates that tapering of the lumen, low intramural friction, and pressure might be important conducive features to the antegrade longitudinal motion of the arterial wall in vivo.

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Automatic Measurements of Diameter, Distension and Intima Media Thickness of the Aorta in Premature Rabbit Pups Using B-Mode Images

Cited by 10 publications

References 20 publications

Response of the carotid artery longitudinal motion to submaximal physical activity in healthy humans—Marked changes already at low workload

Response of the carotid artery longitudinal motion to submaximal physical activity in healthy humans—Marked changes already at low workload

Technology Dependence of Intima-Media Thickness Ultrasonographic Measurements

The impact of geometry, intramural friction, and pressure on the antegrade longitudinal motion of the arterial wall: A phantom and finite element study

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