Automatic neutrophil nucleus lobe counting based on graph representation of region skeleton

Abstract

Abnormal neutrophil nucleus lobation (like left shift and right shift) helps to diagnose for some clinical conditions. Currently, quantification of it depends on the manual microscopic inspection of blood smears by clinicians. The quality of the manual inspection is extremely limited by the efficiency of clinicians and their medical background. This article proposed an automatic lobe counting method based on the graph representation of the nucleus region skeletons. Skeletons of the segmented nucleus regions are computed by augmented Fast Marching Method and transformed into plane graphs. Then the nucleus lobes are split based on the extracted graph properties including width distribution along the skeleton and graph structure decomposition. Experiments show that the proposed method could efficiently approaches the real lobe numbers in blood smears and reliably distinguish the stabs from the segmented neutrophils, thus it should be helpful in automatic neutrophil lobe number quantification and abnormal lobation diagnosis. © 2012 International Society for Advancement of Cytometry

Image analysis of neutrophil nuclear morphology: Learning about phenotypic range and its reliable analysis from patients with pelger‐Huët‐anomaly and treated with colchicine

“…Generally, the analysis is made by biomedical technologists under the microscope by counting the number of nuclear segments according to the rule of threads and the rule of thirds. Thus, the new methods for fully automatic registration of neutrophi ls nuclear shape (18)(19)(20)(21) should combine both lobe counting according to the rule of thirds and calculation of the circularity index based on the automatically reconstructed boundaries of the lobes (nuclear perimeter) and their area. Interestingly, almost all reports of pseudo PHA (hyposegmentation) were described before the mid 1980's.…”

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