Machining center on-line inspecting technology is an effective means that can improve precision and efficiency of machining center, which effectively integrates machining and inspection, makes machining center with partly inspecting function of coordinate measuring machines and improves the degree of inspecting automation compared with manual inspection and off-line inspection. Machining center on-line inspection system based on workpiece has been successfully developed and applied in SSK-U6035 five-axis machining center for palm inspection and control. The application result shows that machining center on-line inspection system based on workpiece can realize integration of machining and inspection more efficiently, increase precision and production efficiency, and reduce the rate of rejection and tool damage. At the same time, the on-line inspection system increases the additional function of the machining center for the manufacturer of machining center, improves the automation degree of the inspection and provides a new inspecting approach for other specific type’s workpiece.