Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents

Romm, Horst; Ainsbury, Elizabeth A.; Barnard, Stephen; Barrios, Leonardo; Barquinero, Joan Francesc; Beinke, Christina; Deperas‐Kaminska, Marta; Grégoire, Éric; Koivistoinen, A; Lindholm, Carita; Moquet, Jayne; Oestreicher, Ursula; Puig, Roser; Rothkamm, Kai; Sommer, Sylwester; Thierens, Hubert; Vandersickel, Veerle; Vral, Anne; Wójcik, Andrzej

doi:10.1016/j.mrgentox.2013.05.013

Cited by 80 publications

(99 citation statements)

References 19 publications

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“…When a new dosimetry technique is proposed, it is necessary to develop, test and then validate the method before it can be said to reach a sufficient standard for inclusion as a fully operational dosimetry method. Inter-comparison exercises are a recognised method for validating tools and techniques within the field of biological and physical retrospective dosimetry (Badie et al 2013;Romm et al 2013;Ainsbury et al 2014;Abend et al 2016).…”

Section: Integration Of New Biological and Physical Retrospective Dosmentioning

confidence: 99%

“…Upon receipt, each partner processed the samples and scored aberrations according to their own standard operating procedures with minor variations between the laboratories, but all according to the recommendations of the IAEA (2011) and refined during the MULTIBIODOSE project (Romm et al 2013) and the two quality controlled exercises in the frame of RENEB ). In brief, whole blood was mixed with a suitable medium (either F10-Ham, RPMI, PB-Max or MEM -various suppliers e.g.…”

Section: Dicentric Assaymentioning

confidence: 99%

“…Following automated metaphase finding (MSearch), cells were either scored manually or dicentric candidates were detected by the DCScore software from high resolution images at 63Â magnification (with oil, AutoCapt) and then assessed by a scorer. Each participants' own preferred classifier was used (Vaurijoux et al 2009;Romm et al 2013). Standardised scoring sheets were provided for both manual and semi-automated scoring modes, and standard scoring procedures were also applied.…”

Section: Dicentric Assaymentioning

confidence: 99%

See 2 more Smart Citations

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiationinduced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2017 VOL. 93, NO. 1, 99-109 http://dx.doi.org/10.1080/09553002.2016 Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.

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Section: Integration Of New Biological and Physical Retrospective Dosmentioning

confidence: 99%

Section: Dicentric Assaymentioning

confidence: 99%

Section: Dicentric Assaymentioning

confidence: 99%

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Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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“…A large amount of work has shown that analysis of the number of dicentrics in 50 cells is sufficient for this purpose (e.g. Romm et al 2013) and thus, for the comparisons within study, 50 cells was chosen for all assays. However, it should be noted that recommendations differ between the assays.…”

Section: Discussionmentioning

confidence: 99%

Uncertainty of fast biological radiation dose assessment for emergency response scenarios

Ainsbury

Higueras

Puig

et al. 2016

International Journal of Radiation Biology

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Purpose: Reliable dose estimation is an important factor in appropriate dosimetric triage categorization of exposed individuals to support radiation emergency response. Materials and methods: Following work done under the EU FP7 MULTIBIODOSE and RENEB projects, formal methods for defining uncertainties on biological dose estimates are compared using simulated and real data from recent exercises.Results: The results demonstrate that a Bayesian method of uncertainty assessment is the most appropriate, even in the absence of detailed prior information. The relative accuracy and relevance of techniques for calculating uncertainty and combining assay results to produce single dose and uncertainty estimates is further discussed. Conclusions: Finally, it is demonstrated that whatever uncertainty estimation method is employed, ignoring the uncertainty on fast dose assessments can have an important impact on rapid biodosimetric categorization. ARTICLE HISTORY

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“…The field of activity of biodosimetry has significantly expanded with the methods of genomics [1,2], proteomics [3,4], metabolomics [5] and transcriptomics [6]. At the same time, methods of cytogenetics that require the cultivation of lymphocytes in vitro or the use of special equipment still remain the classical methods [7][8][9][10][11][12][13]. These include the dicentric chromosome assay (DCA), the micronucleus assay or cytokinesis-block micronucleus assay (CBMN), the premature chromosome condensation assay (PCC), fluorescence in situ hybridization (FISH) and the recent addition of cH2AX scoring as a cytogenetic tool.…”

Section: Introductionmentioning

confidence: 99%

Nuclear Abnormalities of Lymphocytes as the Simplest Markers for Bioindication Test in Case of Mass Casualty Events Involving Radiation Exposure

Kravtsov¹,

Livanova²,

Starkova³

2017

Emerg Med

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Radiation exposure leads to a large number of victims who seek medical help in the first hours. To provide medical care, it is necessary to correctly establish the fact and dose of radiation by bioindication and biodosimetry methods. Lymphocytes containing nuclear anomalies are easily detected cells of peripheral blood and are suitable as objects of bioindication in the case of radioactive exposure to a large number of people. Among these anomalies we distinguish micronuclei, "tailed" nuclei, nucleoplasmatic bridges, dumbbell-shaped nuclei, etc. This review observes the main types of these nuclear anomalies of lymphocytes in the light of their common origin from dicentric chromosomes. We recommend using these alterations in peripheral blood lymphocytes nuclei as the simplest biomarkers in the framework of bioindication tests when it is necessary to work with a large number of victims.

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Automatic scoring of dicentric chromosomes as a tool in large scale radiation accidents

Cited by 80 publications

References 19 publications

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Uncertainty of fast biological radiation dose assessment for emergency response scenarios

Nuclear Abnormalities of Lymphocytes as the Simplest Markers for Bioindication Test in Case of Mass Casualty Events Involving Radiation Exposure

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