Universal bus interface is very helpful for improving the popularity of the device or equipment on both SoC integration and board level computing system design. Because of the diversity of bus interface accessing protocol, universality is usually unable to be achieved in a common interface design. Through introducing the concept of sequence configuration, a method of designing universal bus interface was proposed in this paper. The concept of the bus accessing sequence configuration was analyzed, the specification definition of the universal interface was elaborated and then the architecture was discussed. As a case study, a universal bus writer was implemented and embedded in a FPGA based computing system in real use. Application status shows that the universal bus interface designed through this method can be configured into various kinds of bus interface access timing with distinct cost advantage.