Amplitude variation with offset (AVO) analysis is an 1 efficient tool for hydrocarbon detection and identification of elastic rock properties and fluid types. It has been applied in the present study using reprocessed pre-stack 2D seismic data (1992, Caulerpa) from north-west of the Bonaparte Basin, Australia. The AVO response along the 2D pre-stack seismic data in the Laminaria High NW shelf of Australia was also investigated. Three hypotheses were suggested to investigate the AVO behaviour of the amplitude anomalies in which three different factors; fluid substitution, porosity and thickness (Wedge model) were tested. The AVO models with the synthetic gathers were analysed using log information to find which of these is the controlling parameter on the AVO analysis. AVO cross plots from the real pre-stack seismic data reveal AVO class IV (showing a negative intercept decreasing with offset). This result matches our modelled result of fluid substitution for the seismic synthetics. It is concluded that fluid substitution is the controlling parameter on the AVO analysis and therefore, the high amplitude anomaly on the seabed and the target horizon 9 is the result of changing the fluid content and the lithology along the target horizons. While changing the porosity has little effect on the amplitude variation with offset within the AVO cross plot. Finally, results from the wedge models show that a small change of thickness causes a change in the amplitude; however, this change in thickness gives a different AVO characteristic and a mismatch with the AVO result of the real 2D pre-stack seismic data. Therefore, a constant thin layer with changing fluids is more likely to be the cause of the high amplitude anomalies.