Automation of cell culture assays using a 3D-printed servomotor-controlled microfluidic valve system

Winkler, Steffen; Menke, Jannik; Meyer, Katharina V.; Kortmann, Carlotta; Bahnemann, Janina

doi:10.1039/d2lc00629d

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…In addition, the integration of sensors, e.g., for the detection of cell metabolites, as previously demonstrated by Siller et al [35] and Arshavsky-Graham et al [36], is also feasible. Last but not least, the device presents a promising starting point for the realization of complex fluid flow patterns by integrating valve systems into the setup for automated addition of media supplements, for example, based on the work of Winkler et al [37].…”

Section: Discussionmentioning

confidence: 99%

3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures

Meyer,

Winkler,

Lienig

et al. 2023

Cells

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The use of three-dimensional (3D) cell cultures has become increasingly popular in the contexts of drug discovery, disease modelling, and tissue engineering, as they aim to replicate in vivo-like conditions. To achieve this, new hydrogels are being developed to mimic the extracellular matrix. Testing the ability of these hydrogels is crucial, and the presented 3D-printed microfluidic perfusion system offers a novel solution for the parallel cultivation and evaluation of four separate 3D cell cultures. This system enables easy microscopic monitoring of the hydrogel-embedded cells and significantly reduces the required volumes of hydrogel and cell suspension. This cultivation device is comprised of two 3D-printed parts, which provide four cell-containing hydrogel chambers and the associated perfusion medium chambers. An interfacing porous membrane ensures a defined hydrogel thickness and prevents flow-induced hydrogel detachment. Integrated microfluidic channels connect the perfusion chambers to the overall perfusion system, which can be operated in a standard CO2-incubator. A 3D-printed adapter ensures the compatibility of the cultivation device with standard imaging systems. Cultivation and cell staining experiments with hydrogel-embedded murine fibroblasts confirmed that cell morphology, viability, and growth inside this cultivation device are comparable with those observed within standard 96-well plates. Due to the high degree of customization offered by additive manufacturing, this system has great potential to be used as a customizable platform for 3D cell culture applications.

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Section: Discussionmentioning

confidence: 99%

3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures

Meyer,

Winkler,

Lienig

et al. 2023

Cells

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“…The materials suitable for MJM include photocurable plastic resin, MED610 photopolymer, and VisiJet M3 Crystal. [52][53][54] FDM has become more and more mature and has important applications in many fields. In 1988, Scott Crump invented FDM.…”

Section: Selection Of 3d Printing Techniques For Biosensors and Biome...mentioning

confidence: 99%

Engineering Biosensors and Biomedical Detection Devices from 3D-Printed Technology

et al. 2023

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Limitation of 3D construction ability, complex preparation process, and developing customer demands have promoted efforts to find low-cost, rapid prototyping, and operation simple methods to produce novel functional devices in the near future. Amongst various techniques, 3D-printed technology is a promising candidate for the fabrication of biosensors and biomedical detection devices with a wide variety of potential applications. This review offers four important 3D printing techniques towards biosensors and biomedical detection devices and its applications. The principle and printing process of 3D-printed technologies will be generalized and the printing performance of many 3D printer will be compared. Despite the limitation of 3D-printed resolution, these 3D-printed technologies have already shown promising applications in many biosensors and biomedical detection devices such as 3D-printed microfluidic devices, 3D-printed optical devices, 3D-printed electrochemical devices and 3D-printed integrated devices. Some of the most representative examples will also be discussed here, demonstrating that the 3D-printed technology can rationally design biosensor and biomedical detection devices and achieve important applications in microfluidic, optical, electrochemical and integrated devices.

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“…The 21st century commenced with the ascent of open-source and cost-effective software/hardware solutions, leading to the introduction of electronic prototyping tools like the Arduino microcontroller board for applications in fluid flow control systems [13][14][15][16][17][18][19][20] and droplet microfluidics [21], along with the Raspberry Pi single-board computers [22] used in colour and fluorescence detection, direct cellular image-detection systems and microscopy, and droplet microfluidics. Open-source hardware has been used for several aspects of microfluidic research and development, including pump and flow systems [13][14][15][16][17]19,20,[23][24][25][26][27][28][29] and temperature control [30]. However, imaging is a key component of biological research-from microscopy to tracking optical readouts-and although there are many diverse approaches to sensing of biosystems, optical-based biosensors are one of the most widely used [30,31].…”

Section: Raspberry Pi/pi Camera Systemmentioning

confidence: 99%

Open Hardware for Microfluidics: Exploiting Raspberry Pi Singleboard Computer and Camera Systems for Customisable Laboratory Instrumentation

Sarıyer,

Edwards,

Needs

2023

Biosensors

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The integration of Raspberry Pi miniature computer systems with microfluidics has revolutionised the development of low-cost and customizable analytical systems in life science laboratories. This review explores the applications of Raspberry Pi in microfluidics, with a focus on imaging, including microscopy and automated image capture. By leveraging the low cost, flexibility and accessibility of Raspberry Pi components, high-resolution imaging and analysis have been achieved in direct mammalian and bacterial cellular imaging and a plethora of image-based biochemical and molecular assays, from immunoassays, through microbial growth, to nucleic acid methods such as real-time-qPCR. The control of image capture permitted by Raspberry Pi hardware can also be combined with onboard image analysis. Open-source hardware offers an opportunity to develop complex laboratory instrumentation systems at a fraction of the cost of commercial equipment and, importantly, offers an opportunity for complete customisation to meet the users’ needs. However, these benefits come with a trade-off: challenges remain for those wishing to incorporate open-source hardware equipment in their own work, including requirements for construction and operator skill, the need for good documentation and the availability of rapid prototyping such as 3D printing plus other components. These advances in open-source hardware have the potential to improve the efficiency, accessibility, and cost-effectiveness of microfluidic-based experiments and applications.

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Automation of cell culture assays using a 3D-printed servomotor-controlled microfluidic valve system

Abstract: We present a 3D-printed microfluidic valve system for automated liquid handling in cell culture. The published 3D models enable the customization by the scientific community in a DIY approach.

Cited by 7 publications

References 34 publications

3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures

3D-Printed Microfluidic Perfusion System for Parallel Monitoring of Hydrogel-Embedded Cell Cultures

Engineering Biosensors and Biomedical Detection Devices from 3D-Printed Technology

Open Hardware for Microfluidics: Exploiting Raspberry Pi Singleboard Computer and Camera Systems for Customisable Laboratory Instrumentation

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